{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:23:48Z","timestamp":1788269028267,"version":"build-2803163510"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF22OC0079323"],"award-info":[{"award-number":["NNF22OC0079323"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF19OC0054454"],"award-info":[{"award-number":["NNF19OC0054454"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"crossref","award":["354906"],"award-info":[{"award-number":["354906"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Archaea constitute a diverse group of organisms, many of which inhabit extreme environments, such as haloarchaea that dominate hypersaline ecosystems, like solar salterns. Sampling of solar salterns and other hypersaline environments has resulted in numerous haloarchaeal isolates, including 3 classified and 27 uncharacterized\n                      <jats:italic toggle=\"yes\">Halogranum<\/jats:italic>\n                      species. However, no complete genome has so far been reported for any member of this genus. Here, we present the first comprehensive study of\n                      <jats:italic toggle=\"yes\">Halogranum<\/jats:italic>\n                      sp. SS5-1 isolated from a solar saltern in Samut Sakhon, Thailand.\n                      <jats:italic toggle=\"yes\">Hgn<\/jats:italic>\n                      . SS5-1 is a pleomorphic, aerobic heterotroph that thrives in high salinity and moderate temperature and is capable of hydrolyzing starch. Its genome consists of a 3.6 Mbp chromosome and seven additional plasmids. Based on our phylogenetic analyses, which establish\n                      <jats:italic toggle=\"yes\">Hgn<\/jats:italic>\n                      . SS5-1 as a distinct species, we propose that it will be classified as the novel species\n                      <jats:italic toggle=\"yes\">Halogranum roseipondis<\/jats:italic>\n                      sp. nov. SS5-1\n                      <jats:sup>T<\/jats:sup>\n                      . Additionally, we report that\n                      <jats:italic toggle=\"yes\">Hgn. roseipondis<\/jats:italic>\n                      sp. nov. SS5-1\n                      <jats:sup>T<\/jats:sup>\n                      is infected by\n                      <jats:italic toggle=\"yes\">Hagravirus capitaneum<\/jats:italic>\n                      (HGTV-1), the only virus known to infect a\n                      <jats:italic toggle=\"yes\">Halogranum<\/jats:italic>\n                      host. HGTV-1 exhibits a unique head-tailed morphology and encodes the largest archaeal virus double-stranded DNA genome known to date, including 34 tRNA-encoding genes. Codon usage analysis of the viral genome suggests partial alignment with host preferences, yet the abundance of viral tRNA genes hints at broader roles, potentially including roles in translation and host regulation. This study establishes\n                      <jats:italic toggle=\"yes\">Hgn. roseipondis<\/jats:italic>\n                      and HGTV-1 as a novel virus\u2013host system, opening avenues to explore infection dynamics and the roles of virus-encoded tRNA in archaea.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Archaea that thrive in high-salinity environments are key players in geochemical cycles and important contributors to ecosystem productivity. Despite their ecological significance and importance for the development of novel methodologies in synthetic biology, haloarchaea remain poorly studied. Further exploration of haloarchaea is required to obtain valuable information on the evolution of cellular complexity and the molecular mechanisms that allow cells to thrive in harsh environmental conditions. Here, we present the characterization of a novel archaeon,\n                      <jats:italic toggle=\"yes\">Halogranum roseipondis<\/jats:italic>\n                      sp. SS5-1\n                      <jats:sup>T<\/jats:sup>\n                      , alongside the infection cycle of its associated virus,\n                      <jats:italic toggle=\"yes\">Hagravirus capitaneum<\/jats:italic>\n                      . This tailed myovirus carries an extraordinary set of 34 viral tRNA genes, a feature that opens intriguing questions about virus\u2013host interactions and translational control. Our findings lay the groundwork for future investigations into the expression and function of viral tRNAs in an archaeal model system, thereby opening a new frontier for studying archaeal translation and virus-driven modulation of host cellular processes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00854-26","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T13:03:45Z","timestamp":1786021425000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["The halophilic archaeon\n                    <i>Halogranum roseipondis<\/i>\n                    sp. nov. is susceptible to a virus carrying an exceptionally high number of viral tRNA genes"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9734-4218","authenticated-orcid":false,"given":"Mirka","family":"Lampi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"RNAcious Laboratory, Department of Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5063-8290","authenticated-orcid":false,"given":"Erika","family":"Nordman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"RNAcious Laboratory, Department of Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-8845","authenticated-orcid":false,"given":"Pavlina","family":"Gregorova","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"RNAcious Laboratory, Department of Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki","place":["Helsinki, Finland"]},{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"Doctoral Programme in Integrative Life Science, University of Helsinki Doctoral School, University of Helsinki","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8578-7230","authenticated-orcid":false,"given":"Janne J.","family":"Ravantti","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4400-3601","authenticated-orcid":true,"given":"L. Peter","family":"Sarin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"RNAcious Laboratory, Department of Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Helsinki","place":["Helsinki, Finland"]},{"id":[{"id":"https:\/\/ror.org\/040af2s02","id-type":"ROR","asserted-by":"publisher"}],"name":"HiLIFE \u2013 Helsinki Institute of Life Science, University of Helsinki","place":["Helsinki, Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-3-814"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0967-0645(97)00081-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-014-0670-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2002.tb00900.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02603.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7041902"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002480000069"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1893\/0005-3155(2005)076[0089:RAAPAO]2.0.CO;2"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01891"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.070136-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-016-0660-2"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.014928-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01815-12"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.024976-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-024-01337-3"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4161\/rna.24045"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2020.05.007"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10112106"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/md20080524"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2011.03.007"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1139\/m78-119"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(86)80155-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.625599"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04294.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1047-8477(02)00581-6"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1995.1032"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00572-13"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.01.004"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.612239"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506758102"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msz284"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002516"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64483-0"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.125"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9173-0_1"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.42"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01215-8"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45196-0"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PLAS-0027-2014"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy075"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00019-17"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.204.Supp.93.2"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00875-25"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07874-3"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.237"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/260909"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1257470"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14604"},{"key":"e_1_3_3_51_2","volume-title":"The Halohandbook","author":"Dyall-Smith M","year":"2009","unstructured":"Dyall-Smith M. 2009. The Halohandbook. https:\/\/haloarchaea.com\/wp-content\/uploads\/2018\/10\/Halohandbook_2009_v7.3mds.pdf."},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btn578"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae241"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000760"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-9525(00)02024-2"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(11)80174-8"},{"key":"e_1_3_3_58_2","volume-title":"Manual of clinical microbiology","author":"Carroll KC","year":"2023","unstructured":"Carroll KC, Pfaller MA, Landry ML, McAdam AJ, Patel R. 2023. Edited by B. S. Pritt. Manual of clinical microbiology. 13th ed. American Society for Microbiology, Washington, DC.","edition":"13"},{"key":"e_1_3_3_59_2","unstructured":"Dela Cruz TEE Torres JMO. 2012. Gelatin hydrolysis test protocol. ASM Washington DC. Available from: https:\/\/asm.org\/asm\/media\/protocol-images\/gelatin-hydrolysis-test-protocol.pdf. Retrieved 20 Sep 2024."},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7343-9_3"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00854-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00854-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:09:34Z","timestamp":1788268174000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00854-26"}},"subtitle":[],"editor":[{"given":"Zhe","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":59,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,1]]}},"alternative-id":["10.1128\/spectrum.00854-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00854-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"2026-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00854-26"}}