{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:39Z","timestamp":1787016699199,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Bdellovibrio<\/jats:italic>\n are obligate predators and have been described as living antibiotics since their predatory lifestyle enables them to kill pathogenic bacteria, making\n Bdellovibrio<\/jats:italic>\n a promising biological control agent.\n Bdellovibrio<\/jats:italic>\n strains were isolated from sampling sites in an urban watershed and tested for their killing activity on\n Salmonella enterica<\/jats:italic>\n strains associated with human infections. An enrichment technique was used to isolate\n Bdellovibrio<\/jats:italic>\n from surface water samples from local tributaries to the Oconee River, employing\n Salmonella<\/jats:italic>\n Infantis as the prey bait. The isolated\n Bdellovibrio<\/jats:italic>\n strains were sequenced; their prey range activity was tested against a panel of clinically significant\n S. enterica<\/jats:italic>\n serovars, while predation efficiency was tested on\n S<\/jats:italic>\n . Infantis. The result demonstrated the ability of two newly isolated periplasmic\n Bdellovibrio bacteriovorus<\/jats:italic>\n strains,\n B. bacteriovorus<\/jats:italic>\n YOA24 and\n B. bacteriovorus<\/jats:italic>\n YOA38, to kill 11 antibiotic-resistant\n S. enterica<\/jats:italic>\n serovars and effectively reduce\n S<\/jats:italic>\n . Infantis populations. The strains were identified as periplasmic\n Bdellovibrio<\/jats:italic>\n and members of\n B. bacteriovorus<\/jats:italic>\n based on phenotypic, microscopic, and genotypic characterization.\n B. bacteriovorus<\/jats:italic>\n YOA 24 and YOA38 demonstrated the ability to reduce\n S<\/jats:italic>\n . Infantis by 2 logs in 24 h. The killing activity of\n B. bacteriovorus<\/jats:italic>\n YOA24 and YOA38 indicates their potential to manage\n Salmonella<\/jats:italic>\n outbreaks. Therefore,\n B. bacteriovorus<\/jats:italic>\n strains YOA24 and YOA38 can be considered for development as therapeutic agents or probiotics against\n Salmonella<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n IMPORTANCE<\/jats:title>\n \n Bdellovibrio<\/jats:italic>\n is the most studied obligate predatory bacteria. It has potential for use as biological control of gram-negative bacteria in health, agriculture, and the food industry. Most basic research and applications use the type strain\n Bdellovibrio bacteriovorus<\/jats:italic>\n HD100 or a closely related strain 109J. Screening for other\n Bdellovibrio<\/jats:italic>\n strains and their killing activity should be explored, knowing that prey range and efficiency could differ among\n Bdellovibrio<\/jats:italic>\n strains. This study presents two newly isolated\n B. bacteriovorus<\/jats:italic>\n strains, YOA24 and YOA38, with lytic activity on\n Salmonella enterica<\/jats:italic>\n serovars.\n B. bacteriovorus<\/jats:italic>\n YOA24 and YOA38 represent a biological control agent for foodborne\n S. enterica<\/jats:italic>\n serovars due to their killing activity on the important\n Salmonella<\/jats:italic>\n strains tested.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00861-25","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T13:00:15Z","timestamp":1757941215000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Antibacterial activity of two newly isolated\n Bdellovibrio bacteriovorus<\/i>\n strains on\n Salmonella enterica<\/i>\n serovars of food safety concern"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7641-6681","authenticated-orcid":true,"given":"Yewande O.","family":"Ajao","sequence":"first","affiliation":[{"name":"Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, US Department of Agriculture","place":["Athens, USA"]},{"id":[{"id":"https:\/\/ror.org\/040vxhp34","id-type":"ROR","asserted-by":"publisher"}],"name":"Oak Ridge Institute for Science and Education","place":["Oak Ridge, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lari M.","family":"Hiott","sequence":"additional","affiliation":[{"name":"Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, US Department of Agriculture","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9536-324X","authenticated-orcid":true,"given":"Laura E.","family":"Williams","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Georgia Institute of Technology","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlene R.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, US Department of Agriculture","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8500-3395","authenticated-orcid":true,"given":"Jonathan G.","family":"Frye","sequence":"additional","affiliation":[{"name":"Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, US Department of Agriculture","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2015.0220"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph17091122"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/phar.1636"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2021.1908956"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063397"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s44259-024-00048-1"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2212650120"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuad057"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00662"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03094-22"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9402073"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005608"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006248"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000861"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz943"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00832"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/lambio\/ovad088"},{"key":"e_1_3_4_20_2","unstructured":"CDC. 2024. Salmonella Infection (Salmonellosis). Available from: https:\/\/www.cdc.gov\/salmonella\/about\/index.html"},{"key":"e_1_3_4_21_2","first-page":"137","volume-title":"Advances in food and nutrition research","author":"Cesare A","year":"2018","unstructured":"CesareA. 2018. Salmonella in foods: a reemerging problem, p 137\u2013179. In Advances in food and nutrition research"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/21553769.2015.1051243"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2018.2493"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2012.45.2.71"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00426-11"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-58.2.160"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00393-22"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-670"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.382"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02046064"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2021.1979464"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00591-19"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2021.103878"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-005-0038-6"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090816-093618"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc07b01s26"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00508-17"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2020.103572"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00861-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00861-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:07:15Z","timestamp":1759842435000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00861-25"}},"subtitle":[],"editor":[{"given":"Catherine M.","family":"Logue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,7]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10,7]]}},"alternative-id":["10.1128\/spectrum.00861-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00861-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,7]]},"assertion":[{"value":"2025-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00861-25"}}