{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:42:23Z","timestamp":1785422543683,"version":"3.56.0"},"reference-count":102,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Congenital transmission of\n                      <jats:italic toggle=\"yes\">Trypanosoma cruzi<\/jats:italic>\n                      is a significant public health concern, involving complex interactions between the parasite, the placenta, and the fetus. However, the mechanisms facilitating parasite passage across the placental barrier remain incompletely understood. This study explores the transcriptional response of both\n                      <jats:italic toggle=\"yes\">T. cruzi<\/jats:italic>\n                      (TcI) and human trophoblastic cells during\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      infection, aiming to provide insights into parasite-host interactions relevant to congenital Chagas disease. Two TcI strains (MHOM\/CO\/01\/DA and MHOM\/CO\/04\/MG) were used to infect BeWo cells, and RNA was sequenced at 72 and 120 h post-infection. The MG strain exhibited higher invasion and replication rates than the DA strain. Transcriptomic analysis of the parasite revealed modulation of 157 genes, including several encoding surface-associated multigene families potentially involved in host interaction. In host cells, the upregulation of histones and ribonucleoproteins suggested chromatin remodeling, while the downregulation of RNA splicing-related genes pointed to alterations in RNA processing. At 120 h, genes associated with necroptosis were upregulated, indicating a shift in the host cell response. Overall, these results highlight key molecular changes during trophoblast infection by TcI strains and provide a transcriptomic resource for advancing the understanding of congenital\n                      <jats:italic toggle=\"yes\">T. cruzi<\/jats:italic>\n                      infection.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>\n                        <jats:bold>IMPORTANCE<\/jats:bold>\n                      <\/jats:title>\n                      <jats:p>\n                        Congenital transmission of\n                        <jats:italic toggle=\"yes\">Trypanosoma cruzi<\/jats:italic>\n                        , the causative agent of Chagas disease, remains a significant global health concern. However, the mechanisms by which the parasite crosses the placenta and impacts fetal development are not yet fully understood. This study investigates the interaction between\n                        <jats:italic toggle=\"yes\">T. cruzi<\/jats:italic>\n                        and trophoblastic cells\u2014key components of the placental barrier\u2014by analyzing transcriptomic changes in both the parasite and the host during infection. The findings provide insight into the molecular alterations associated with congenital infection and contribute to a better understanding of the host-parasite dynamics at the maternal-fetal interface. These results offer a valuable resource for advancing research into the biological processes underlying congenital Chagas disease.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00887-25","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:00:31Z","timestamp":1760961631000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Dual transcriptomics suggests trophoblastic sequestration and splicing blockade induced by\n                    <i>Trypanosoma cruzi<\/i>\n                    during\n                    <i>in vitro<\/i>\n                    infection"],"prefix":"10.1128","volume":"13","author":[{"given":"Tatiana M.","family":"C\u00e1ceres","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0108mwc04","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Microbiolog\u00eda and Biotecnolog\u00eda-UR (CIMBIUR), School of Sciences and Engineering, Universidad del Rosario","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lissa","family":"Cruz-Saavedra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0108mwc04","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Microbiolog\u00eda and Biotecnolog\u00eda-UR (CIMBIUR), School of Sciences and Engineering, Universidad del Rosario","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz Helena","family":"Pati\u00f1o","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0108mwc04","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Microbiolog\u00eda and Biotecnolog\u00eda-UR (CIMBIUR), School of Sciences and Engineering, Universidad del Rosario","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-2500","authenticated-orcid":true,"given":"Manuel Alfonso","family":"Patarroyo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01j543957","id-type":"ROR","asserted-by":"publisher"}],"name":"Grupo de Investigaci\u00f3n B\u00e1sica en Biolog\u00eda Molecular e Inmunolog\u00eda (GIBBMI), Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC)","place":["Bogot\u00e1, Colombia"]},{"id":[{"id":"https:\/\/ror.org\/059yx9a68","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia","place":["Bogot\u00e1, Colombia"]},{"id":[{"id":"https:\/\/ror.org\/01h2taq97","id-type":"ROR","asserted-by":"publisher"}],"name":"Health Sciences Faculty, Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A)","place":["Bogot\u00e1, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-9312","authenticated-orcid":true,"given":"Juan David","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0108mwc04","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaciones en Microbiolog\u00eda and Biotecnolog\u00eda-UR (CIMBIUR), School of Sciences and Engineering, Universidad del Rosario","place":["Bogot\u00e1, Colombia"]},{"id":[{"id":"https:\/\/ror.org\/032db5x82","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Global Health and Inter-Disciplinary Research, Department of Global, Environmental and Genomic Health Sciences, College of Public Health, University of South Florida","place":["Tampa, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"World Health Organization. 2017. Integrating neglected tropical diseases into global health and development: fourth WHO report on neglected tropical diseases [Internet]. World Health Organization. Available from: https:\/\/apps.who.int\/iris\/handle\/10665\/255011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1410150"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.12396"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.74"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.70.201"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2019.165591"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.07.016"},{"key":"e_1_3_2_10_2","first-page":"84","article-title":"Placental lesions in human Trypanosoma cruzi infection","volume":"38","author":"Fernandez-Aguilar S","year":"2005","unstructured":"Fernandez-Aguilar S, Lambot M-A, Torrico F, Alonso-Vega C, C\u00f3rdoba M, Suarez E, No\u00ebl J-C, Carlier Y. 2005. Placental lesions in human Trypanosoma cruzi infection. Rev Soc Bras Med Trop 38 Suppl 2:84\u201386.","journal-title":"Rev Soc Bras Med Trop"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-020-00210-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00282.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01854"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.08.022"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.09.021"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2010.05.007"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2014.09.017"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2004.07.079"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146947"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7834-2_1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0508035103"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2018.02.011"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-015-0083-3"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.01073.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02504-22"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2988-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005436"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2018.07.001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12634"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.12-0086"},{"key":"e_1_3_2_31_2","first-page":"513","article-title":"The first case of congenital Chagas\u2019 disease analyzed by AP-PCR in Colombia","volume":"29","author":"Pavia PX","year":"2009","unstructured":"Pavia PX, Montilla M, Fl\u00f3rez C, Herrera G, Ospina JM, Manrique F, Nicholls RS, Puerta C. 2009. The first case of congenital Chagas\u2019 disease analyzed by AP-PCR in Colombia. Biomedica 29:513\u2013522.","journal-title":"Biomedica"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.4606"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19-0340"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005511"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-298"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-495"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1351"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000613"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2852"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-238"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159197"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.692134"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2017.08.021"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107483"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-024-08183-4"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp851"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1092"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13398"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-97602010000300002"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2011.07.003"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002476"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0516"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002271"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006222"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003458"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.760830"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.04.002"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5198-4"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00242-24"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes11101196"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.07.005"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2023.102085"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25978"},{"key":"e_1_3_2_68_2","unstructured":"Lima R de Bulla ACS Silva M da. 2023. Functional annotation hypothetical proteins: a world to be explored in drug development in Trypanosomatids. bioRxiv"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02319-22"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003176"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-370"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00348"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025914"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01341"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3751403"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-9-68"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.3390\/life11060534"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000864"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2014.11.016"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-911"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1563-8"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04125-y"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20051226"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00165-14"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00058.2017"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-013-9469-7"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.07.008"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.09.005"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046767"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1042\/bse0510015"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes11091085"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1255"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12056"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.718028"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.010"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-53589-w"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08467"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2004.09.005"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2020.04.040"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioad002"},{"key":"e_1_3_2_101_2","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20211577"},{"key":"e_1_3_2_102_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00630-3"},{"key":"e_1_3_2_103_2","first-page":"88","article-title":"Actors of necroptosis scenario in cell\u2019s scene","volume":"21","author":"Nikseresht S","year":"2017","unstructured":"Nikseresht S, Khodagholi F, Ahmadiani A. 2017. Actors of necroptosis scenario in cell\u2019s scene. Physiol Pharmacol 21:88\u2013101.","journal-title":"Physiol Pharmacol"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00887-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00887-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T14:07:13Z","timestamp":1764684433000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00887-25"}},"subtitle":[],"editor":[{"given":"Denis","family":"Sereno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":102,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12,2]]}},"alternative-id":["10.1128\/spectrum.00887-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00887-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,2]]},"assertion":[{"value":"2025-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00887-25"}}