{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T17:14:30Z","timestamp":1781198070618,"version":"3.54.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"MOST | National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2016YFD0500701"],"award-info":[{"award-number":["2016YFD0500701"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Earmarked Fund for Modern Agro-industry Technology Research System of China","award":["CARS-35"],"award-info":[{"award-number":["CARS-35"]}]},{"name":"The Special Fund for Henan Agriculture Research System","award":["S2012-06-02"],"award-info":[{"award-number":["S2012-06-02"]}]},{"name":"Science and Technology Innovation Team of Henan Academy of Agricultural Sciences","award":["2021TD02"],"award-info":[{"award-number":["2021TD02"]}]},{"name":"Science and Technology Innovation Team of Henan Academy of Agricultural Sciences","award":["2022TD02"],"award-info":[{"award-number":["2022TD02"]}]},{"name":"Science and Technology Development Project of Henan province","award":["222102110453"],"award-info":[{"award-number":["222102110453"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,10,26]]},"abstract":"<jats:p>Classical swine fever (CSF) is a World Organisation for Animal Health (formerly the Office International des Epizooties [OIE]) notifiable terrestrial and aquatic animal disease, causing great economic losses to the swine industry worldwide during the past decades. Due to the use of the most effective and safe attenuated live vaccine for CSF prevention, differentiation of infected and vaccinated pigs is vital work, as well as a bottleneck for eradication of CSF.<\/jats:p>","DOI":"10.1128\/spectrum.00891-22","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T14:00:43Z","timestamp":1664460043000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Differentiation of Classical Swine Fever Virus Virulent and Vaccine Strains by CRISPR\/Cas13a"],"prefix":"10.1128","volume":"10","author":[{"given":"Yuhang","family":"Zhang","sequence":"first","affiliation":[{"name":"International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmei","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruining","family":"Wang","sequence":"additional","affiliation":[{"name":"Henan University of Animal Husbandry and Economy, College of Veterinary Medicine, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Wang","sequence":"additional","affiliation":[{"name":"International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxu","family":"Xing","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanmin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Public Health and Preventive Medicine Teaching and Research Center, Henan University of Chinese Medicine, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wan","sequence":"additional","affiliation":[{"name":"International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqing","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3834-9975","authenticated-orcid":true,"given":"Gaiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China"},{"name":"Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China"},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.12.006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aivir.2015.03.002"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1135(00)00141-3"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.09.008"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.04.004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-017-3464-4"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.12.006"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00859-13"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.906-912.2001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.468-471.2005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.07.033"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.04.046"},{"key":"e_1_3_3_14_2","first-page":"847","article-title":"Method of discrimination between vaccine type and wild type viruses of classical swine fever","volume":"83","author":"Cho HS","year":"2006","unstructured":"Cho HS, Lee HI, Park NY. 2006. Method of discrimination between vaccine type and wild type viruses of classical swine fever. Indian Vet J 83:847\u2013849.","journal-title":"Indian Vet J"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.184"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5573"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19802"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9321"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8836"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01696"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12989"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-020-02497-9"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mim.2019.11.008"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2019.1664939"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b01526"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00209"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c08165"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.0c04303"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1039\/d0cc06412b"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18615-1"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.01.008"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx580"},{"key":"e_1_3_3_36_2","first-page":"27","article-title":"Development and application of a RT-nest PCR assay for differential detection of C-strain and wild-type viruses of classical swine fever virus","volume":"2","author":"Wang HG","year":"2013","unstructured":"Wang HG, Ning YB, Ying C, Liu C, Wei RR, Gong WZ. 2013. Development and application of a RT-nest PCR assay for differential detection of C-strain and wild-type viruses of classical swine fever virus. J Sustain Agr 2:27\u201340.","journal-title":"J Sustain Agr"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4880-5687","authenticated-orcid":false,"given":"Hua-Ji","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8993-7494","authenticated-orcid":false,"given":"Qin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00891-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00891-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T13:56:05Z","timestamp":1666792565000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00891-22"}},"subtitle":[],"editor":[{"given":"Biao","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,10,26]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,10,26]]}},"alternative-id":["10.1128\/spectrum.00891-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00891-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,26]]},"assertion":[{"value":"2022-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00891-22"}}