{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:28:49Z","timestamp":1761719329117,"version":"3.41.2"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["2022YFD18070101"],"award-info":[{"award-number":["2022YFD18070101"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Bovine herpesvirus (BoHV) infection poses a significant threat to the healthy development of the cattle industry. BoHV-1 primarily causes infectious bovine rhinotracheitis, while BoHV-5 is associated with bovine necrotic meningoencephalitis. These two pathogens not only exhibit a high correlation in antigenicity and genetic background but, more importantly, can establish latent infections within the bovine ganglion. Therefore, establishing a rapid and convenient pathogen detection method for BoHV-1 and BoHV-5 is crucial for early diagnosis, epidemic monitoring, and minimizing economic losses. In this study, utilizing the conserved sequences of BoHV-1 and BoHV-5 as targets, we developed a rapid and visually accessible recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay suitable for on-site detection. This method enables visual detection within 30 min for clinical samples containing as little as 1 tissue culture infectious dose 50 (TCID\n              <jats:sub>50<\/jats:sub>\n              ) and performs comparably to qPCR, with slightly lower sensitivity. The findings of this research offer new insights for early clinical detection and the control of BoHV-1 and BoHV-5 infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Bovine herpesvirus (BoHV), a significant respiratory pathogen in cattle, is widely prevalent worldwide, posing a substantial economic threat to the cattle industry. In this study, we have developed a rapid on-site detection method for BoHV, termed RPA-LFD, which can carry out pathogen detection with a sensitivity of no less than 1 TCID\n              <jats:sub>50<\/jats:sub>\n              within 30 min. When combined with screening, isolation, and culling measures, this approach can effectively eliminate the sources of BoHV infection, providing a scientifically feasible solution for BoHV prevention and control.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00895-24","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T14:01:27Z","timestamp":1737986487000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Rapid, sensitive, and visible RPA-LFD assay for BoHV-1 and BoHV-5"],"prefix":"10.1128","volume":"13","author":[{"given":"Yue","family":"Wu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenci","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyang","family":"Yi","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuijing","family":"He","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changmin","family":"Hu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guishan","family":"Ye","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0037-5916","authenticated-orcid":true,"given":"Anding","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production","place":["Wuhan, China"]},{"name":"Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People\u2019s Republic of China","place":["Wuhan, China"]},{"name":"International Research Center for Animal Disease, Ministry of Science and Technology of the People\u2019s Republic of China","place":["Wuhan, China"]},{"name":"Guangdong Provincial Key Laboratory of Research on the Technology of Pig-breeding and Pig-disease Prevention","place":["Guangzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2016.1006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2017.00189"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006059"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-022-09887-7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1993.54.04.534"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-4-437"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-5877(00)00128-8"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170136"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(96)00165-X"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.02.012"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.07.005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11030577"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.11.025"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114343"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-017-1284-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01859-23"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.841875"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2022.2042669"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-021-00997-9"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1017\/S146625230700134X"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.07.014"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2015.10.005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2018.07.024"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-017-1324-9"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02796-22"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.11.080"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2023.01.009"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.1c00620"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.05.040"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00269-7"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.882796"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20154243"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81969-0"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00895-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00895-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T14:05:10Z","timestamp":1741097110000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00895-24"}},"subtitle":[],"editor":[{"given":"Luciana Jesus","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,4]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,4]]}},"alternative-id":["10.1128\/spectrum.00895-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00895-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"type":"electronic","value":"2165-0497"}],"subject":[],"published":{"date-parts":[[2025,3,4]]},"assertion":[{"value":"2024-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00895-24"}}