{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:52:14Z","timestamp":1786117934298,"version":"build-2736575974"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009837","name":"Canadian Food Inspection Agency","doi-asserted-by":"publisher","award":["in-house funding"],"award-info":[{"award-number":["in-house funding"]}],"id":[{"id":"10.13039\/100009837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mammalian\n                      <jats:italic toggle=\"yes\">Campylobacter fetus<\/jats:italic>\n                      (CF) is divided into two subspecies,\n                      <jats:italic toggle=\"yes\">C. fetus fetus<\/jats:italic>\n                      (CFF) and\n                      <jats:italic toggle=\"yes\">C. fetus venerealis<\/jats:italic>\n                      (CFV), the latter being bovine-adapted and responsible for the notifiable disease bovine genital campylobacteriosis (BGC). Differentiation between CF subspecies has traditionally been undertaken by a few biochemical tests, but these are complicated by the existence of a biotype,\n                      <jats:italic toggle=\"yes\">C. fetus venerealis<\/jats:italic>\n                      intermedius (CFVi), which shares attributes of both CFF and CFV. Molecular methods targeting specific genes have gained acceptance for more accurate subtype identification and align well with whole-genome analysis. However, limited genomic diversity between subtypes has confounded efforts to understand the genetic basis for differential host tropism and pathogenesis of these organisms. A previous study of a small cohort of\n                      <jats:italic toggle=\"yes\">C. fetus<\/jats:italic>\n                      isolates suggested that\n                      <jats:italic toggle=\"yes\">dam<\/jats:italic>\n                      gene coding variations might correlate with CF subtype. Accordingly, this study examines a cohort of 331\n                      <jats:italic toggle=\"yes\">C. fetus<\/jats:italic>\n                      genomes, representative of all seven phylogenetic groups for their complement of adenine methylases and the genomic motifs they target in representative isolates. All CF isolates retained a\n                      <jats:italic toggle=\"yes\">cfeM1<\/jats:italic>\n                      gene, the presence of which correlates with RAATTY methylation, while seven other adenine methylase genes exhibited distinct cladal distributions. Notably, a\n                      <jats:italic toggle=\"yes\">cjeM1<\/jats:italic>\n                      gene appears to target the CCAN7TAG\/CTAN7TGG motif in CFV and CFVi isolates only. Given the increasing recognition of the impact of adenine methylation on bacterial-host interactions, further exploration of the role of adenine methylation in\n                      <jats:italic toggle=\"yes\">C. fetus<\/jats:italic>\n                      pathogenesis could reveal mechanisms contributing to BGC and thus aid in its eradication.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Campylobacter fetus<\/jats:italic>\n                        remains an important zoonotic pathogen, for which a better understanding of its host tropism and pathogenesis is sought. However, the limited genomic variation observed between subtypes has to date confounded efforts in this regard. This study suggests that an alternative approach that examines epigenetic differences between subtypes, specifically adenine methylation patterns, may reveal mechanisms critical to the pathologies of these organisms.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00954-26","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T01:19:26Z","timestamp":1782868766000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Lineage-associated differences in adenine methylation patterns of mammalian-associated\n                    <i>Campylobacter fetus<\/i>\n                    isolates: a possible role for epigenetic factors in host tropism and pathogenesis"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6659-6861","authenticated-orcid":true,"given":"Susan","family":"Nadin-Davis","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qxr8t08","id-type":"ROR","asserted-by":"publisher"}],"name":"Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2130-0427","authenticated-orcid":true,"given":"Marc-Olivier","family":"Duceppe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qxr8t08","id-type":"ROR","asserted-by":"publisher"}],"name":"Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8795-2973","authenticated-orcid":true,"given":"Mingsong","family":"Kang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qxr8t08","id-type":"ROR","asserted-by":"publisher"}],"name":"Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Hoover","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qxr8t08","id-type":"ROR","asserted-by":"publisher"}],"name":"Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Devenish","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qxr8t08","id-type":"ROR","asserted-by":"publisher"}],"name":"Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.057778-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw146"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1556\/EuJMI.2.2012.1.10"},{"key":"e_1_3_4_5_2","unstructured":"Florent A. 1959. Les deux vibriosis g\u00e9nitales; la vibriose due \u00e0 V. fetus venerealis et la vibriose d\u00f2rigine intestinale due \u00e0 V. fetus intestinalis.: Mededelingen der Veeartsenijschool van de Rijks. Universiteit te Gent"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1194243"},{"key":"e_1_3_4_7_2","first-page":"1031","volume-title":"Manual of diagnostic tests and vaccines for terrestrial animals","author":"OIE","year":"2018","unstructured":"OIE. 2018. Bovine genital campylobacteriosis, p 1031\u20131044. In Manual of diagnostic tests and vaccines for terrestrial animals, 8th ed. 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