{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:40Z","timestamp":1786117900035,"version":"build-2736575974"},"reference-count":91,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Nanyang City Science and Technology Research Project","award":["25JCQY059"],"award-info":[{"award-number":["25JCQY059"]}]},{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"crossref","award":["252102311173"],"award-info":[{"award-number":["252102311173"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Henan Provincial Medical Science and Technology Tackling Program","award":["SBGJ202503055"],"award-info":[{"award-number":["SBGJ202503055"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      remains a critical global health concern, particularly in low- and middle-income countries, owing to its high carriage of diverse virulence factor genes (VFGs) and multidrug resistance. Here, we analyzed the prevalence and genotype distribution of VFGs in\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      isolated from Nanyang Central Hospital (NYCH), China, along with their antibiotic susceptibility profiles. The study involved 79 multidrug-resistant (MDR) and 96 non-MDR (nMDR) strains and the following 11 key VFGs (\n                      <jats:italic toggle=\"yes\">nan1<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">pilA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">aprA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">phzS<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">plcH<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">toxA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">lasB<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">exoS<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">exoU<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">exoT<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">exoY<\/jats:italic>\n                      ). Antibiogram profiling showed near-universal resistance to at least one antibiotic class. MDR strains exhibited 23 distinct antibiotic resistance patterns, compared with 13 patterns in nMDR strains. A moderate negative correlation was observed between \u03b2-lactams\/\u03b2-lactamase inhibitors and fluoroquinolone resistance. Moreover, statistical analysis revealed that the\n                      <jats:italic toggle=\"yes\">phzS<\/jats:italic>\n                      gene displayed the most significant prevalence difference between MDR and nMDR subgroups (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). Finally, 28 distinct genotypes were identified in nMDR isolates, and 26 genotypes appeared in MDR strains. There was a statistically significant correlation found between the presence of genotype XX (r = 0.481,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001) and genotype XXVIII (r = \u22120.320,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001) in two subgroups. Collectively, our findings provide data support for understanding the pathogenic mechanism of local\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      , and are of great significance for guiding clinical anti-infection treatment and infection control.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                        is an opportunistic pathogen notorious for causing difficult-to-treat infections, particularly among hospitalized patients in resource-limited healthcare settings. This study analyzed clinical isolates from a major hospital in China to investigate the prevalence and genotypic distribution of virulence factor genes (VFGs) and antibiotic susceptibility profiles of\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        . Our results showed that multidrug-resistant (MDR) strains displayed more complex and diverse resistance profiles than non-MDR (nMDR) strains. Both MDR and nMDR isolates exhibited high genotypic diversity. Notably, a statistically significant association was observed between genotype XX and genotype XXVIII across the two subgroups. These findings elucidate the molecular basis underlying the variable treatment difficulty of\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        infections and offer valuable insights for guiding empirical antibiotic selection. Characterizing local bacterial features, including antibiotic susceptibility profiles and the distribution of clinically relevant virulence factors, may improve understanding of regional resistance trends and support antimicrobial stewardship and infection control efforts.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00973-26","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:01:06Z","timestamp":1782738066000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and genotypic distribution of virulence factor genes and antibiotic susceptibility profiles in clinical\n                    <i>Pseudomonas aeruginosa<\/i>\n                    isolates from a public hospital in Nanyang, China"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6527-5650","authenticated-orcid":false,"given":"Si-Ping","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00s528j33","id-type":"ROR","asserted-by":"publisher"}],"name":"Central Laboratory, Nanyang Central Hospital","place":["Nanyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Yu","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00s528j33","id-type":"ROR","asserted-by":"publisher"}],"name":"Central Laboratory, Nanyang Central Hospital","place":["Nanyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00s528j33","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical laboratory, Nanyang Central Hospital","place":["Nanyang, 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