{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T17:03:39Z","timestamp":1784653419127,"version":"3.55.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Timely and accurate identification of yeasts is essential for adequate treatment, considering the increase in antifungal resistance of some species, particularly for\n              <jats:italic>C. auris<\/jats:italic>\n              . Current matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) manufacturer\u2019s protocol for identification of yeasts requires 24- to 72-h cultivation on Sabouraud dextrose media (SAB), but not some of the mainstay primary culture media used in mycology such as inhibitory mold agar (IMA), Mycosel, CHROMagar Candida Plus, and CHROMagar Candida. As culture media can influence MALDI-TOF MS identification results, this study evaluated the accuracy and performance of identification of clinically relevant yeasts on these first-line media using the VITEK-MS MALDI-TOF MS system.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                In this study, a panel of 140 strains (21 species) was used to assess the performance of the selected media. Although not in the manufacturer\u2019s list of accepted media, IMA and chromogenic media are suitable for the identification of yeasts on the VITEK-MS systems. CHROMagar Candida Plus allowed the identification of 135\/140 isolates tested after 24-h incubation similar to SAB reference media (137\/140). Yeast isolates that grew on Mycosel selective media were also reliably identified by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry. VITEK-MS system with IVD database V3.2 correctly identified\n                <jats:italic>C. auris<\/jats:italic>\n                strains to the species level on CHROMagar Candida Plus alleviating the need for subcultivation and reduced turnaround time (24\u201372 h) to identification for patient screening.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00974-24","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T13:05:46Z","timestamp":1724159146000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Performance of common primary and chromogenic culture media for MALDI-TOF MS identification of clinically relevant yeasts"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4356-9702","authenticated-orcid":true,"given":"Simon","family":"L\u00e9vesque","sequence":"first","affiliation":[{"name":"Service de microbiologie, CIUSSS de l'Estrie - Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Qu\u00e9bec, Canada"},{"name":"D\u00e9partement de microbiologie et infectiologie, Facult\u00e9 de m\u00e9decine et des sciences de la sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Brown","sequence":"additional","affiliation":[{"name":"Service de microbiologie, CIUSSS de l'Estrie - Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5871-3933","authenticated-orcid":false,"given":"Philippe J.","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Laboratoire de sant\u00e9 publique du Qu\u00e9bec, Institut national de sant\u00e9 publique du Qu\u00e9bec, Sainte-Anne-de-Bellevue, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Allard","sequence":"additional","affiliation":[{"name":"Service de microbiologie, CIUSSS de l'Estrie - Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Qu\u00e9bec, Canada"},{"name":"D\u00e9partement de microbiologie et infectiologie, Facult\u00e9 de m\u00e9decine et des sciences de la sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2023.2240956"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12020322"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05209-11"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03708-13"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21739"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2012.720720"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2017.03.001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S238002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13119"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13633"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2017.11.002"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-022-00656-3"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00974-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00974-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T13:07:54Z","timestamp":1727960874000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00974-24"}},"subtitle":[],"editor":[{"given":"Vera","family":"Tesic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,10,3]]},"references-count":12,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,3]]}},"alternative-id":["10.1128\/spectrum.00974-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00974-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,3]]},"assertion":[{"value":"2024-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00974-24"}}