{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:08:38Z","timestamp":1785434918460,"version":"3.56.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Rapid spread of carbapenemase-producing Enterobacterales is a global health threat for which tigecycline is one of the few remaining therapeutic options. Resistance by\n tet<\/jats:italic>\n (x4) is particularly worrisome, given its presence on mobile genetic elements. Here we report on a tigecycline-resistant\n Escherichia coli<\/jats:italic>\n strain isolated from a patient returning from Pakistan. A\n tet<\/jats:italic>\n (x4) gene was found on an IncFII plasmid isolated from an ST410\n E. coli<\/jats:italic>\n co-harboring NDM-5. The combination of carbapenemase and\n tet<\/jats:italic>\n (x4) is concerning.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Tigecycline is one of the few last-resort therapeutic options available for treating multidrug-resistant gram-negative bacterial infections, particularly of interest in carbapenemase-producing isolates. Mobile tigecycline resistance is still relatively rare, especially in North America and among clinical isolates. Here we describe the identification of a high-risk clone (\n E. coli<\/jats:italic>\n ST410) in Canada that contains the tigecycline resistance gene\n tet<\/jats:italic>\n (x4) on an IncFII plasmid and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n on a separate IncFIB plasmid. Here, we highlight a rare resistance gene in combination with a carbapenemase, both of which are plasmid mediated, complicating antimicrobial treatment options.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00976-25","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:00:23Z","timestamp":1759928423000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Identification of plasmid-mediated tigecycline resistance\n tet<\/i>\n (x4) and New Delhi metallo-\u03b2-lactamase (NDM) in an\n Escherichia coli<\/i>\n isolate from Canada"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8014-1107","authenticated-orcid":true,"given":"Laura F.","family":"Mataseje","sequence":"first","affiliation":[{"name":"National Microbiology Laboratory","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7914-9198","authenticated-orcid":true,"given":"Nicole","family":"Lerminiaux","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison","family":"McGeer","sequence":"additional","affiliation":[{"name":"Mt Sinai Hospital","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennie","family":"Johnstone","sequence":"additional","affiliation":[{"name":"Mt Sinai Hospital","place":["Toronto, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03dbr7087","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, University of Toronto","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Poutanen","sequence":"additional","affiliation":[{"name":"Mt Sinai Hospital","place":["Toronto, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"McGill","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023xf2a37","id-type":"ROR","asserted-by":"publisher"}],"name":"Public Health Agency of Canada","place":["Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Longtin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056jjra10","id-type":"ROR","asserted-by":"publisher"}],"name":"Jewish General Hospital","place":["Montreal, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on behalf of Canadian Nosocomial Infection Surveillance Program (CNISP)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciae403"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02930-19"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0445-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01633-21"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0496-4"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.147613"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2019.09.003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.150689"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020292"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.14745\/ccdr.v44i01a07"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00337-18"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2024.127730"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00976-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00976-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:10:25Z","timestamp":1762265425000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00976-25"}},"subtitle":[],"editor":[{"given":"Rafael","family":"Vignoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"references-count":12,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,4]]}},"alternative-id":["10.1128\/spectrum.00976-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00976-25","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.03.27.645688","asserted-by":"object"}]},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"2025-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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