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Thus far, various\n                      <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                      vaccine candidates have been tested, but many have shown limited immunogenicity, high reactogenicity, or insufficient cross-serotype protection due to serotype-specific O-polysaccharide (OPS) targeting. In this study, we evaluated mRNA vaccine candidates targeting the highly conserved\n                      <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                      invasion plasmid antigens (IpaB and IpaC), aiming to provide a preventive strategy that can address various serogroups of\n                      <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                      . Using a mouse intraperitoneal infection model, we assessed the immune responses and protective efficacy of lipid nanoparticle (LNP)-encapsulated mRNA vaccines. The results demonstrated that the vaccine induced neutralizing antibodies capable of inhibiting intracellular invasion by\n                      <jats:italic toggle=\"yes\">Shigella flexneri<\/jats:italic>\n                      , confirming its potential for protection against this serotype.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study aimed to utilize mRNA vaccine technology targeting conserved antigens (IpaB and IpaC) to overcome the limitations of previous\n                        <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                        vaccines. By demonstrating functional immune responses against\n                        <jats:italic toggle=\"yes\">Shigella flexneri<\/jats:italic>\n                        , our findings suggest that mRNA vaccines may serve as a preventive strategy against\n                        <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                        infection and contribute to the foundation for future research.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00993-25","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T14:16:45Z","timestamp":1756822605000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Neutralizing antibodies induced by IpaB and IpaC mRNA vaccines inhibit\n                    <i>Shigella flexneri<\/i>\n                    invasion"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5808-1664","authenticated-orcid":true,"given":"Ye-Eun","family":"Moon","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Health Sciences and Technology, GAIHST, Gachon University","place":["Incheon, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"An","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bio-medical Sciences, GAIHST, Gachon University","place":["Incheon, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3024-3439","authenticated-orcid":false,"given":"Pil-Gu","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Science, Gachon University","place":["Seongnam, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee-Jong","family":"Hong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Health Sciences and Technology, GAIHST, Gachon University","place":["Incheon, Korea"]},{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Gachon University","place":["Incheon, Korea"]},{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Lee Gil Ya Cancer and Diabetes Institute, Gachon University","place":["Incheon, Korea"]},{"id":[{"id":"https:\/\/ror.org\/03ryywt80","id-type":"ROR","asserted-by":"publisher"}],"name":"Korea mRNA Vaccine Initiative, Gachon University","place":["Seongnam, Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.esp-0006-2017"},{"key":"e_1_3_4_3_2","volume-title":"Mandell, Douglas, and Bennett\u2019s principles and practice of infectious diseases","author":"Mandell GL","year":"2010","unstructured":"Mandell GL, Bennett JE, Dolin R. 2010. 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