{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T10:52:00Z","timestamp":1776941520214,"version":"3.51.4"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["1RO1DE020100"],"award-info":[{"award-number":["1RO1DE020100"]}]},{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["F32DE027281"],"award-info":[{"award-number":["F32DE027281"]}]},{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["K99DE031018"],"award-info":[{"award-number":["K99DE031018"]}]},{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["R00DE031018"],"award-info":[{"award-number":["R00DE031018"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Understanding the molecular mechanisms governing microbial interactions is crucial for unraveling the complexities of microbial communities and their ecological impacts. Here, we employed a two-species model system comprising the oral bacteria\n              <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>\n              and\n              <jats:italic>Streptococcus gordonii<\/jats:italic>\n              to investigate how synergistic and antagonistic interactions between microbes impact their resilience to environmental change and invasion by other microbes. We used an\n              <jats:italic>in vitro<\/jats:italic>\n              colony biofilm model and focused on two\n              <jats:italic>S<\/jats:italic>\n              .\n              <jats:italic>gordonii<\/jats:italic>\n              -produced extracellular molecules, L-lactate and H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              , which are known to impact fitness of this dual-species community. While the ability of\n              <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n              to cross-feed on\n              <jats:italic>S. gordonii<\/jats:italic>\n              -produced L-lactate enhanced its fitness during co-culture, this function showed little impact on the ability of co-cultures to resist environmental change. In fact, the ability of\n              <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n              to catabolize L-lactate may be detrimental in the presence of tetracycline, highlighting the complexity of interactions under antimicrobial stress. Furthermore, H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              , known for its antimicrobial properties, had negative impacts on both species in our model system. However, H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              production by\n              <jats:italic>S. gordonii<\/jats:italic>\n              enhanced\n              <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n              tolerance to tetracycline, suggesting a protective role under antibiotic pressure. Finally,\n              <jats:italic>S. gordonii<\/jats:italic>\n              significantly inhibited the bacterium\n              <jats:italic>Serratia marcescens<\/jats:italic>\n              from invading\n              <jats:italic>in vitro<\/jats:italic>\n              biofilms, but this inhibition was lost during co-culture with\n              <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n              and in a murine abscess model, where\n              <jats:italic>S. gordonii<\/jats:italic>\n              actually promoted\n              <jats:italic>S. marcescens<\/jats:italic>\n              invasion. These data indicate that microbial interactions can impact fitness of a bacterial community upon exposure to stresses, but these impacts are highly environment dependent.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Microbial interactions are critical modulators of the emergence of microbial communities and their functions. However, how these interactions impact the fitness of microbes in established communities upon exposure to environmental stresses is poorly understood. Here, we utilized a two-species community consisting of\n              <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>\n              and\n              <jats:italic>Streptococcus gordonii<\/jats:italic>\n              to examine the impact of synergistic and antagonistic interactions on microbial resilience to environmental fluctuations and susceptibility to microbial invasion. We focused on the\n              <jats:italic>S. gordonii<\/jats:italic>\n              -produced extracellular molecules, L-lactate and H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              , which have been shown to mediate interactions between these two microbes. We discovered that seemingly beneficial functions, such as\n              <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n              cross-feeding on\n              <jats:italic>S. gordonii<\/jats:italic>\n              -produced L-Lactate, can paradoxically exacerbate vulnerabilities, such as susceptibility to antibiotics. Moreover, our data highlight the context-dependent nature of microbial interactions, emphasizing that a seemingly potent antimicrobial, such as H\n              <jats:sub>2<\/jats:sub>\n              O\n              <jats:sub>2<\/jats:sub>\n              , can have both synergistic and antagonistic effects on a microbial community dependent on the environment.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01005-24","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T13:17:09Z","timestamp":1726233429000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Microbial interactions impact stress tolerance in a model oral community"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1189-6696","authenticated-orcid":true,"given":"Gina R.","family":"Lewin","sequence":"first","affiliation":[{"name":"School of Biological Sciences and Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Emory-Children\u2019s Cystic Fibrosis Center, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma R.","family":"Evans","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Emory-Children\u2019s Cystic Fibrosis Center, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4933-9983","authenticated-orcid":true,"given":"Marvin","family":"Whiteley","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Center for Microbial Dynamics and Infection, Georgia Institute of Technology, Atlanta, Georgia, USA"},{"name":"Emory-Children\u2019s Cystic Fibrosis Center, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11121731"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00683-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro928"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.1.177-207.2001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.09.006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00024-07"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-014-4067-3"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2015.8"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1996.tb00181.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2212340119"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.79"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.1998.tb00694.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000155018.67835.1a"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6055-6063.2005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1999.tb02395.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002012"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809533106"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400586111"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00782-16"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01737194"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.25.14547"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.12.3770-3783.2001"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1369-5274(98)80147-1"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.29.3.1013-1020.1980"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/11.1.46"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00794.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7292(95)80010-a"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.13028"},{"key":"e_1_3_3_30_2","volume-title":"Molecular biology of streptococci","author":"Chhatwal S","year":"2007","unstructured":"Chhatwal S. 2007. 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