{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:55:08Z","timestamp":1793746508142,"version":"4.6.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100015850","name":"Digestive Medical Coordinated Development Center of Beijing Hospitals Authority","doi-asserted-by":"publisher","award":["XXT22"],"award-info":[{"award-number":["XXT22"]}],"id":[{"id":"10.13039\/501100015850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7222060"],"award-info":[{"award-number":["7222060"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005047","name":"Liaoning Natural Science Foundation","doi-asserted-by":"crossref","award":["2020JH2\/10300041"],"award-info":[{"award-number":["2020JH2\/10300041"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Beijing Hospitals Authority's Ascent Plan","award":["DFL20221003"],"award-info":[{"award-number":["DFL20221003"]}]},{"name":"Beijing Hope Run Special Fund of Cancer Foundation of China","award":["LC2021A06"],"award-info":[{"award-number":["LC2021A06"]}]},{"DOI":"10.13039\/501100005089","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7222153"],"award-info":[{"award-number":["7222153"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Capital Health Development Research Project","award":["Shoufa 2020-1-2024"],"award-info":[{"award-number":["Shoufa 2020-1-2024"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,2,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In China, 45% of adolescents with obesity develop fatty liver disease, a condition that increases the long-term risk of developing cirrhosis and liver cancer. Although the factors triggering nonalcoholic fatty liver disease (NAFLD) vary in children, the composition of intestinal microflora has been found to play an increasingly important role. However, evidence is limited on the prevalence of nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH) in Chinese children. Therefore, this study aimed to evaluate the fecal microbiome of Chinese children with NAFLD and further analyze the potential of flora in regulating NAFLD-related symptoms and metabolic functions. Specifically, the study applied a 16S rRNA and metagenomic sequencing to the fecal samples of pediatric patients with NAFLD, NASH, and NAFL, as well as healthy controls, to explore the correlation among NAFLD-related indexes, metabolic pathways, and gut flora. The findings showed that some fecal microbiota had a negative correlation with body mass index, and various NAFLD-related bacteria, including\n            <jats:italic>Lachnoclostridium<\/jats:italic>\n            ,\n            <jats:italic>Escherichia-Shigella<\/jats:italic>\n            , and\n            <jats:italic>Faecalibacterium prausnitzii<\/jats:italic>\n            , were detected. Consequently, the study concluded that the variation in gut microbiota might be more important in improving NAFLD\/NASH compared with single species, providing a microbiota diagnostic profile of NAFLD\/NASH.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>This study aims to characterize the gut microbiota in Chinese children with nonalcoholic fatty liver disease (NAFLD) through 16S rRNA and metagenomic sequencing. The results highlight the association between fecal microbiota and NAFLD in Chinese children, demonstrating distinct characteristics compared to adults and children from other countries. Based on the sequencing data from our cohort\u2019s fecal samples, we propose a microbiota model with a high area under the curve for distinguishing between NAFLD and healthy individuals. Furthermore, our follow-up study reveals that changes in the relative abundance of microbial biomarkers in this model are consistent with variations in patients\u2019 body mass index. These findings suggest the potential utility of the microbiota model and microbial biomarkers for diagnosing and treating NAFLD in children.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01006-23","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T14:00:58Z","timestamp":1704722458000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Role of intestinal flora in the development of nonalcoholic fatty liver disease in children"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-239X","authenticated-orcid":false,"given":"Jing","family":"Zhang","sequence":"first","affiliation":[{"name":"National Center for Children\u2019s Health, Beijing Children\u2019s Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7971-3753","authenticated-orcid":false,"given":"Mengxuan","family":"Shi","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunna","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Center for Children\u2019s Health, Beijing Children\u2019s Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4767-7698","authenticated-orcid":false,"given":"Guangcai","family":"Liang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"},{"name":"Department of Scientific Research, Microvita Medical Technology Co., Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Li","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"},{"name":"Department of Scientific Research, Microvita Medical Technology Co., Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Ge","sequence":"additional","affiliation":[{"name":"Microbial Resources and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caixia","family":"Pei","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory 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