{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:18:35Z","timestamp":1785417515867,"version":"3.56.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370543"],"award-info":[{"award-number":["32370543"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan University Medical Research Fund","award":["YDYXJJ2024-0045"],"award-info":[{"award-number":["YDYXJJ2024-0045"]}]},{"name":"International Science and Technology Cooperation-Yunnan International Science and Technology Cooperation Base","award":["No. 202003AE140004"],"award-info":[{"award-number":["No. 202003AE140004"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19AI089672"],"award-info":[{"award-number":["U19AI089672"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Applied Basic Research Projects-Union Foundation","award":["202101AY070001-108"],"award-info":[{"award-number":["202101AY070001-108"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360118"],"award-info":[{"award-number":["32360118"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yunnan Applied Basic Research Projects-Union Foundation","award":["202301AY070001-116"],"award-info":[{"award-number":["202301AY070001-116"]}]},{"name":"Guangxi Zhuang Autonomous Region Health Commission of Scientific Research Project","award":["ZA20231282"],"award-info":[{"award-number":["ZA20231282"]}]},{"name":"Guangxi Zhuang Autonomous Region Health Commission of Scientific Research Project","award":["ZA20221274"],"award-info":[{"award-number":["ZA20221274"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      acetyl-CoA synthetase (PfAcAS) is an important source of acetyl-CoA. We detected mutations S868G and V950I in PfAcAS by whole-genome sequencing analysis in certain recrudescent parasites after treatment with artesunate and dihydroartemisinin-piperaquine. Using CRISPR\/Cas9 technology, we engineered parasite lines to carry the PfAcAS S868G and V950I mutations in two genetic backgrounds and evaluated their susceptibilities to antimalarial drugs\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . The results demonstrated that PfAcAS S868G and V950I mutations alone or in combination affected the susceptibility of\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      to several antimalarial drugs, including the artemisinin derivatives (dihydroartemisinin, artesunate, and artemether) and chloroquine, although absolute changes in susceptibilities were modest.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Malaria, an infectious disease caused by\n                        <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                        parasites and transmitted by mosquitoes, continues to be one of the most pressing public health challenges worldwide.\n                        <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                        has demonstrated reduced sensitivity to artemisinin-based combination therapies (ACTs), thereby intensifying the difficulties associated with malaria management. Currently, only a limited number of molecular markers exist for identifying drug resistance in\n                        <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                        , and these markers do not fully elucidate the mechanisms behind this resistance. In this study, we performed whole-genome sequencing analysis on\n                        <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                        strains that reemerged following ACT treatment. We aim to identify molecules potentially associated with drug resistance, which may provide new molecular markers for monitoring drug resistance in\n                        <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01026-25","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T13:02:51Z","timestamp":1757595771000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Acetyl-CoA synthetase mutations affect the susceptibility of\n                    <i>Plasmodium falciparum<\/i>\n                    to antimalarial drugs"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8006-3487","authenticated-orcid":true,"given":"Wei","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/038c3w259","id-type":"ROR","asserted-by":"publisher"}],"name":"Yunnan Provincial Key Laboratory of Public Health and Biosafety & Department of Pathogen Biology and Immunology, Faculty of Basic Medical Science, Kunming Medical University","place":["Kunming, China"]},{"id":[{"id":"https:\/\/ror.org\/05tr94j30","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, the Affiliated Hospital of Yunnan University","place":["Kunming, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Xiang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/038c3w259","id-type":"ROR","asserted-by":"publisher"}],"name":"Yunnan Provincial Key Laboratory of Public Health and Biosafety & Department of 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