{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:43:24Z","timestamp":1768265004966,"version":"3.49.0"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Pseudovirus-based assays are efficient and accessible platforms for measuring neutralizing antibody titers and screening antiviral drugs against enveloped viruses. They typically employ two types of packaging systems, retroviral-based or vesicular stomatitis virus (VSV)-based systems. These systems have been validated for several high-risk pathogens, including Ebola, Marburg, Dengue and, more recently, SARS-CoV-2, as they offer a safe alternative for studying high-containment viruses. In a recent study (Wu X, Liu F, Li T, Li D, Shen Y, Zhang X, Liu S, Jiang Q, Zhao C, Nie J, Wang Y, Feng B, Liu W, Huang W, Microbiol Spectr, 13:e01557-24, 2025,\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/spectrum.01557-24\" xlink:type=\"simple\">https:\/\/doi.org\/10.1128\/spectrum.01557-24<\/jats:ext-link>\n              ), Wu and colleagues developed a pseudovirus system and optimized it for neutralization assays targeting the newly identified Dehong virus (DEHV). The authors also utilized this system to detect bioluminescent signals in mouse models. Importantly, using this pseudovirus platform, they confirmed that Niemann-Pick C1 (NPC1) functions as the entry receptor for DEHV, consistent with prior findings using infectious viruses. This work provides valuable insights into the limited understanding of DEHV biology.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01035-25","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T13:50:05Z","timestamp":1753797005000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["A tool of the trade for a newly identified filovirus: pseudovirus-based system for Dehong virus entry and neutralization"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7478-5547","authenticated-orcid":true,"given":"Banhi","family":"Biswas","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health","place":["Baltimore, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","volume-title":"Filoviridae: Marburg and Ebola viruses","author":"Feldmann H","year":"2013","unstructured":"Feldmann H, Sanchez A, Geisbert TW. 2013. Filoviridae: Marburg and Ebola viruses. In , Sixth Edition. Wolters Kluwer Health Adis (ESP)."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2313789121"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01557-24"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.4.3155-3160.1998"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/382658"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1963"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-03154-6"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2022.126993"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.06.026"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113366"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01035-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01035-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T13:04:35Z","timestamp":1756818275000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01035-25"}},"subtitle":[],"editor":[{"given":"Alexander","family":"Bello","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":10,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,2]]}},"alternative-id":["10.1128\/spectrum.01035-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01035-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01035-25"}}