{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:57:48Z","timestamp":1790812668535,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100006706","name":"Tampereen Tuberkuloosis\u00e4\u00e4ti\u00f6","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006706","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Competitive State Research Financing of the Expert Responsibility Area of NordLab","award":["KT0016"],"award-info":[{"award-number":["KT0016"]}]},{"name":"Fimlab","award":["51409"],"award-info":[{"award-number":["51409"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,11,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Dermatophytes cause superficial infections of skin, hair, and nails. Even though they rarely cause severe infections, they are relatively common, particularly in primary health care. Diagnosis of dermatophyte infections has relied on relatively slow culture-based methods. Nucleic acid-based detection (PCR) methods might provide results more rapidly. Here, we describe the transition from culture-based methods into PCR-based methods in Northern Finland with a catchment area of approximately 720,000 mostly Caucasian people. This study included 14,330 samples collected between 2019 and 2022. Our results showed that the PCR-based method has become the diagnostic test of choice for these infections in this area. Commercial real-time PCR assay DermaGenius 2.0 complete multiplex test detected more positive results than culture and covered the most important dermatophytes,\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , and few less common species. With PCR, the mean turn-around-time from sample request to results decreased from 19 days to 16 hours.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Superficial fungal infections, dermatophytosis, are remarkably common worldwide, affecting an estimated 20%\u201325% of the global population. In the diagnosis of these infections, fast and accurate results by PCR shorten the time to diagnosis and help clinicians to avoid unnecessary antifungal treatments.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01049-24","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T13:05:29Z","timestamp":1726578329000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["PCR enables rapid detection of dermatophytes in practice"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2138-7242","authenticated-orcid":true,"given":"E.","family":"Aho-Laukkanen","sequence":"first","affiliation":[{"name":"Northern Finland Laboratory Center, Nordlab, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"M\u00e4ki-Koivisto","sequence":"additional","affiliation":[{"name":"Northern Finland Laboratory Center, Nordlab, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Torvikoski","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. P.","family":"Sinikumpu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Medical Research Center Oulu, PEDEGO Research Unit, University of Oulu, Oulu University Hospital, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Huilaja","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Medical Research Center Oulu, PEDEGO Research Unit, University of Oulu, Oulu University Hospital, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. S.","family":"Junttila","sequence":"additional","affiliation":[{"name":"Northern Finland Laboratory Center, Nordlab, Oulu, Finland"},{"name":"Research Unit of Biomedicine and Internal Medicine, University of Oulu, Oulu, Finland"},{"name":"Cytokine Biology Research Group, Tampere University, Tampere, Finland"},{"name":"Fimlab Laboratories, Tampere, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdin.2020.10.003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2008.01606.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ski2.53"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099533"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/jgs.16706"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001082"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-016-0073-9"},{"key":"e_1_3_3_9_2","volume-title":"The working group established by the Finnish Medical Society Duodecim and the Finnish Dermatological Society","author":"Skin infections","year":"2021","unstructured":"Skin infections. 2021. Current Care Guidelines. In The working group established by the Finnish Medical Society Duodecim and the Finnish Dermatological Society. Vol. Available from. The Finnish Medical Society Duodecim, Helsinki, Finland."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12153"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2021.115413"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2020.03.002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.08.017"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myad092"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2022.03.019"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof8010011"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.7573\/dic.2023-5-1"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof5010020"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2023.2280137"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy020"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01049-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01049-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T09:39:50Z","timestamp":1730799590000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01049-24"}},"subtitle":[],"editor":[{"given":"Vera","family":"Tesic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,11,5]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11,5]]}},"alternative-id":["10.1128\/spectrum.01049-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01049-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,5]]},"assertion":[{"value":"2024-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01049-24"}}