{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:39:44Z","timestamp":1784036384004,"version":"3.55.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"crossref","award":["18-0084,22-0342"],"award-info":[{"award-number":["18-0084,22-0342"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"crossref","award":["1\/0093\/22"],"award-info":[{"award-number":["1\/0093\/22"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of education, youth and sports of the Czech Republic","award":["LM2023055"],"award-info":[{"award-number":["LM2023055"]}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["23-05622S"],"award-info":[{"award-number":["23-05622S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,12,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              is an emerging opportunistic pathogen affecting patients with chronic lung diseases, primarily cystic fibrosis (CF), or those under immunosuppression. Hence, investigations into the epidemiology and transmission of\n              <jats:italic>M. abscessus<\/jats:italic>\n              and accurate antibiotic susceptibility data are essential for the effective treatment of infections caused by this pathogen. This retrospective nationwide study included all clinical\n              <jats:italic>M. abscessus<\/jats:italic>\n              isolates (\n              <jats:italic>n<\/jats:italic>\n              = 59) from 29 patients diagnosed in the Czech Republic and Slovakia between 2018 and 2023. Whole genome sequencing (WGS) was performed to identify clusters and classify isolates into predominant circulating clones (DCC). Subspecies identification of unique isolates showed subspecies\n              <jats:italic>abscessus<\/jats:italic>\n              as the most prevalent (69.0%). The results of drug-susceptibility testing showed that 65.5% of all isolates were resistant to at least three antibiotics tested. CF patients under 24 years of age were the most at-risk group for\n              <jats:italic>M. abscessus<\/jats:italic>\n              infection. WGS identified seven clusters (including two cross-border) comprising CF and non-CF patients with a total clustering rate of 48.3%. One cluster involved patients infected with subspecies\n              <jats:italic>massiliense<\/jats:italic>\n              strains differing by 0 single nucleotide polymorphisms hospitalized in the same center. Furthermore, we identified representatives of all major DCCs. This study revealed predominant\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              complex clones circulating in the Czech Republic and Slovakia. The results show the high discriminatory power of WGS in the molecular epidemiology of\n              <jats:italic>M. abscessus<\/jats:italic>\n              and provide supporting evidence of direct or indirect cross-transmission of subspecies\n              <jats:italic>massiliense<\/jats:italic>\n              among both CF and non-CF patients.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study highlights the importance of understanding\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              transmission because it poses a growing threat to vulnerable populations, especially young cystic fibrosis patients. Investigating how it spreads and which antibiotics work best is crucial for effective treatment. This research used whole genome sequencing to track\n              <jats:italic>M. abscessus<\/jats:italic>\n              and found evidence of potential transmission between patients, including across borders. The findings suggest that dominant strains are circulating and some patients may be infected through direct or indirect contact. This knowledge can inform infection control and treatment strategies.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01056-24","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T13:02:17Z","timestamp":1729688537000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["High rate of macrolide resistance and closely genetically related\n            <i>Mycobacterium abscessus<\/i>\n            complex strains identified among both cystic fibrosis and non-cystic fibrosis patients within two countries"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2025-2830","authenticated-orcid":true,"given":"Mat\u00fa\u0161","family":"Doh\u00e1l","sequence":"first","affiliation":[{"name":"Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University","place":["Bratislava, Slovakia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u011bra","family":"Dvo\u0159\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Hrom\u00e1dkov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Pinkov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Amlerov\u00e1","sequence":"additional","affiliation":[{"name":"Charles University, Faculty of Medicine in Pilsen, Faculty Hospital","place":["Pilsen, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Spitaleri","sequence":"additional","affiliation":[{"name":"Division of Immunology, Transplantation and Infectious Diseases, Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]},{"name":"Vita-Salute San Raffaele University","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0376-1342","authenticated-orcid":true,"given":"Federico","family":"di Marco","sequence":"additional","affiliation":[{"name":"Division of Immunology, Transplantation and Infectious Diseases, Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute","place":["Milan, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarmila","family":"Hnilicov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences","place":["Prague, Czechia"]},{"name":"Department of Genetics and Microbiology, Faculty of Science, Charles University","place":["Prague, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard","family":"Gond\u00e1\u0161","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Jessenius Faculty of Medicine in Martin, Comenius University","place":["Bratislava, Slovakia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"International Reference Laboratory of Mycobacteriology, Statens Serum Institut","place":["Copenhagen, 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