{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:16:43Z","timestamp":1785417403526,"version":"3.56.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Screening for carbapenemase-producing\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      (CPE) and vancomycin-resistant\n                      <jats:italic toggle=\"yes\">Enterococcus<\/jats:italic>\n                      (VRE) colonization among hospitalized patients is a standard infection control procedure that also guides appropriate antibiotic treatment in healthcare settings. Extensive CPE screening in low-prevalence regions imposes a considerable laboratory workload and substantial costs that can be mitigated through the utilization of pool testing. In this study, we evaluated PCR pooling for the detection of CPE and VRE colonization from rectal swabs collected in our hospital, using the Xpert Carba-R and Xpert\n                      <jats:italic toggle=\"yes\">vanA\/vanB<\/jats:italic>\n                      assays. CPE pooling demonstrated excellent performance, with 94.44% sensitivity (74.2\u201399.0 CI 95%), 100% specificity (99.1\u2013100), 100% positive predictive value (81.6\u2013100), and 99.78% (98.7\u2013100) negative predictive value (NPV). In contrast, VRE detection showed lower performance, with 75.86% (63.5\u201385.0 CI 95%) specificity and 100% (92.0\u2013100) NPV, primarily due to false-positive results for the\n                      <jats:italic toggle=\"yes\">vanB<\/jats:italic>\n                      gene. Overall, the pooling technique showed great potential for CPE screening in low-prevalence settings. Further studies are needed to evaluate VRE pooling and clarify the clinical utility of PCR results for\n                      <jats:italic toggle=\"yes\">vanB<\/jats:italic>\n                      , which currently requires culture confirmation.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Carbapenemase-producing\n                        <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                        (CPE) and vancomycin-resistant enterococci (VRE) pose a major threat to healthcare systems due to their role in hospital-acquired infections and limited treatment options. Routine screening for CPE\/VRE colonization is essential for preventing transmission, yet conventional culture-based methods are labor-intensive and time-consuming. This study demonstrates that PCR pooling is a highly effective and resource-efficient approach for CPE screening in low-prevalence settings, maintaining high sensitivity and specificity while reducing laboratory workload. The performance of VRE pooling, particularly for\n                        <jats:italic toggle=\"yes\">vanB<\/jats:italic>\n                        detection, requires further evaluation. These findings support the adoption of pooling strategies for CPE surveillance and highlight the need for improved molecular diagnostics for VRE, offering a promising solution to address growing challenges in clinical microbiology diagnostics.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01080-25","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T13:01:00Z","timestamp":1756904460000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of sample pooling using Xpert Carba-R and Xpert\n                    <i>vanA<\/i>\n                    \/\n                    <i>vanB<\/i>\n                    PCR for screening of carbapenemase-producing\n                    <i>Enterobacterales<\/i>\n                    and vancomycin-resistant\n                    <i>Enterococcus<\/i>\n                    colonization"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-0850","authenticated-orcid":true,"given":"Danielle","family":"Keidar-Friedman","sequence":"first","affiliation":[{"name":"Emerging Infectious Diseases Research Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]},{"name":"Clinical Microbiology Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Gil","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anka","family":"Tsur","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tal","family":"Brosh-Nissimov","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]},{"name":"Faculty of Health Sciences, Ben Gurion University in the Negev","place":["Beer Sheba, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]},{"id":[{"id":"https:\/\/ror.org\/04mhzgx49","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Medical and Health Sciences, Tel Aviv University","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boaz David","family":"Rosenfeld","sequence":"additional","affiliation":[{"name":"Medison Pharma","place":["Petah Tikva, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadav","family":"Sorek","sequence":"additional","affiliation":[{"name":"Emerging Infectious Diseases Research Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]},{"name":"Clinical Microbiology Laboratory, Samson Assuta Ashdod University Hospital","place":["Ashdod, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00539"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-023-01323-y"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/medsci6010001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0591-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/338461"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix824"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1255381"},{"key":"e_1_3_3_9_2","unstructured":"World Health Organization. 2017. 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