{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:40:00Z","timestamp":1792510800875,"version":"4.5.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The major science and technology projects of china national tobacco corporation","award":["110202201034(XJ-05)"],"award-info":[{"award-number":["110202201034(XJ-05)"]}],"id":[{"id":"https:\/\/ror.org\/030d08e08","id-type":"ROR","asserted-by":"crossref"}]},{"name":"The chief scientist innovation project of state tobacco monopoly administration\/china national tobacco corporation","award":["No. 702024CK0380"],"award-info":[{"award-number":["No. 702024CK0380"]}],"id":[{"id":"https:\/\/ror.org\/030d08e08","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["No. 2022YFF1000700"],"award-info":[{"award-number":["No. 2022YFF1000700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mold contamination causes economic losses and poses risks to human and animal health. Tobacco leaves, as a high-value agricultural product, are particularly susceptible to mold contamination during post-harvest handling. In this study, the antifungal performance of\n                      <jats:italic toggle=\"yes\">Bacillus velezensis<\/jats:italic>\n                      ZD-F13 was evaluated on tobacco leaves. In laboratory-scale assays, ZD-F13 treatment reduced the fungal colony count by 84% relative to the\n                      <jats:italic toggle=\"yes\">Penicillium<\/jats:italic>\n                      group. Electronic-nose and gas chromatography-ion mobility spectrometry analyses showed that the volatile profile of the\n                      <jats:italic toggle=\"yes\">Penicillium<\/jats:italic>\n                      + ZD-F13 group was closer to that of the normal leaves group than to that of the\n                      <jats:italic toggle=\"yes\">Penicillium<\/jats:italic>\n                      group. To further evaluate its practical applicability,\n                      <jats:italic toggle=\"yes\">B. velezensis<\/jats:italic>\n                      ZD-F13 was tested on 5 kg of tobacco leaves and reduced the fungal colony count by 50% relative to the untreated group. Microbial community analysis revealed changes in bacterial and fungal community composition, including an increase in the relative abundance of\n                      <jats:italic toggle=\"yes\">Bacillus<\/jats:italic>\n                      from 0.09% to 48.1%. These findings support the potential application of\n                      <jats:italic toggle=\"yes\">B. velezensis<\/jats:italic>\n                      ZD-F13 for controlling tobacco leaf mold and providing a basis for further development of postharvest biocontrol strategies.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Mold contamination of tobacco leaves causes substantial economic losses and can compromise product quality and safety during postharvest storage and processing. Effective control strategies remain limited, particularly those capable of suppressing mold without disturbing the natural microbial community or altering quality-related characteristics. This study showed that ZD-F13 treatment reduced fungal colony counts under controlled laboratory conditions and was associated with a lower fungal colony count in the 5-kg practical trial. ZD-F13 treatment also shifted the volatile profile toward that of healthy leaves without causing a statistically detectable loss of Shannon diversity under the tested conditions. These findings suggest that ZD-F13 has potential as a postharvest biocontrol agent for tobacco leaves.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01131-26","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:02:06Z","timestamp":1788872526000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Biocontrol of tobacco leaf mold using\n                    <i>Bacillus velezensis<\/i>\n                    : effects on volatile profiles and microbial community"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6005-8944","authenticated-orcid":true,"given":"Daiyuan","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0099xbw16","id-type":"ROR","asserted-by":"publisher"}],"name":"Zhengzhou Tobacco Research Institute of China National Tobacco 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