{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:08:14Z","timestamp":1788570494191,"version":"build-2803163510"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"crossref","award":["2020YFE0204300"],"award-info":[{"award-number":["2020YFE0204300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Pseudomonas juntendi<\/jats:italic>\n              has received limited research attention, yet strains carrying multi-drug resistance genes pose a threat to global public health. We aimed to characterize the genome of two fecal-derived strains of\n              <jats:italic>Pseudomonas juntendi<\/jats:italic>\n              , both harboring\n              <jats:italic>tmexCD-toprJ<\/jats:italic>\n              ,\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>NDM-1<\/jats:sub>\n              , and\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>PME-1<\/jats:sub>\n              on the chromosome, recovered from two patients. Average nucleotide identity (ANI) analysis showed that L4008hy and L4046hy were remarkably similar. They showed high levels of resistance to aztreonam, imipenem, ciprofloxacin, amikacin, piperacillin\u2013tazobactam, ceftazidime\u2013avibactam, and polymyxin B in antimicrobial susceptibility testing using agar dilution method and broth microdilution methods. Additionally, an integrative and conjugative element (ICE) similar to ICE\n              <jats:italic>6660<\/jats:italic>\n              was detected on the chromosome, which contains all resistance genes and has a relatively complete transfer module, and potential transfer mechanisms were identified. Phylogenetic analysis of\n              <jats:italic>P. juntendi<\/jats:italic>\n              reveals the genomic diversity of the species and sheds light on environmental\u2013human transmission.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Up to now, research on\n                <jats:italic>Pseudomonas juntendi<\/jats:italic>\n                is still very limited. Our findings suggest that\n                <jats:italic>P. juntendi<\/jats:italic>\n                commonly carries diversity resistance genes on chromosomes and is stably inherited, highlighting the need for further studies on the antimicrobial properties of this bacterium. The coexistence of\n                <jats:italic>tmexCD-toprJ<\/jats:italic>\n                ,\n                <jats:italic>bla<\/jats:italic>\n                <jats:sub>NDM-1<\/jats:sub>\n                , and\n                <jats:italic>bla<\/jats:italic>\n                <jats:sub>PME-1<\/jats:sub>\n                on the chromosome in\n                <jats:italic>P. juntendi<\/jats:italic>\n                was reported for the first time. The identified integrative and conjugative element (ICE) contains all the identified resistance genes and serves as a vector for resistance gene transfer between bacteria.\n                <jats:italic>P. juntendi<\/jats:italic>\n                , which harbors multi-drug resistance genes, particularly those encoding carbapenemases, acts as a reservoir of resistance genes. Its spread in clinical settings poses additional challenges to treatment.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01136-24","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T14:09:33Z","timestamp":1740492573000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Coexistence of\n            <i>tmexCD-toprJ<\/i>\n            ,\n            <i>bla<\/i>\n            <sub>NDM-1<\/sub>\n            , and\n            <i>bla<\/i>\n            <sub>PME-1<\/sub>\n            in multi-drug-resistant\n            <i>Pseudomonas juntendi<\/i>\n            isolates recovered from stool samples"],"prefix":"10.1128","volume":"13","author":[{"given":"Wugao","family":"Liu","sequence":"first","affiliation":[{"name":"Clinical Laboratory Lishui People\u2019s Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University","place":["Lishui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Jin","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Lishui People\u2019s Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University","place":["Lishui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningjun","family":"Wu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Lishui People\u2019s Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University","place":["Lishui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Wu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Lishui People\u2019s Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University","place":["Lishui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5304-2915","authenticated-orcid":true,"given":"Chunsheng","family":"Qu","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Lishui People\u2019s Hospital, The Sixth Affiliated Hospital of Wenzhou Medical University","place":["Lishui, 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