{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:04:23Z","timestamp":1769731463780,"version":"3.49.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>Seasonal increase of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza virus A\/B (Flu A\/B), and respiratory syncytial virus (RSV) require rapid diagnostic test methods for the management of respiratory tract infections. In this study, we compared the diagnostic accuracy of Savanna RVP4 (RVP4, QuidelOrtho) with Xpert Xpress Plus SARS-CoV-2\/Flu\/RSV (Xpert, Cepheid). Nasopharyngeal swabs from patients treated at a tertiary care hospital (Germany) were tested for SARS-CoV-2, Flu A\/B, and RSV by RVP4 to assess diagnostic accuracy (reference standard: Xpert). The intra and inter assay precision of Ct-values was assessed by repeated test in triplicates (on day 1) and duplicates (days 2\u20133). All patients with a physician\u2019s order for a multiplex test for SARS-CoV-2, Flu, and RSV test were included. Duplicate swabs from the same patient, samples with a total volume \u22641 mL, or inappropriate shipment\/storage were excluded. In total, 229 swabs were included between September 2023 and February 2024. The concordance between both tests was 96.5% (SARS-CoV-2), 98.7% (Flu A), and 99.6% (RSV). Flu B was not detected by both tests. The RVP4 test had a sensitivity of 85%\u201395% and a specificity of 100% for the detection of SARS-CoV-2, Flu A, and RSV. The intra and inter assay precision of Ct-values from RVP4 was 3% and 2% (SARS-CoV-2), 5% and 4% (Flu A), and 0% and 3% (RSV), respectively. The Savanna RVP4 has a favorable diagnostic accuracy for the detection of SARS-CoV-2, Flu A, and RSV.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>We assessed the diagnostic accuracy of a new point-of-care test for the rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza virus A\/B (Flu A\/B), and respiratory syncytial virus (RSV). The new test has a concordance with the reference standard of 96.5% (SARS-CoV-2), 98.7% (Flu A), and 99.1% (RSV). The sensitivity of 85%\u201395% and specificity of 100% for the detection of SARS-CoV-2, Flu A, and RSV is comparable with similar nucleic acid amplification-based point of care tests but at lower costs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01153-24","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T13:19:38Z","timestamp":1720703978000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Diagnostic accuracy of Savanna RVP4 (QuidelOrtho) for the detection of Influenza A virus, RSV, and SARS-CoV-2"],"prefix":"10.1128","volume":"12","author":[{"given":"B\u00fcsra","family":"K\u00f6se","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9168-9290","authenticated-orcid":true,"given":"Frieder","family":"Schaumburg","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)00982-5"},{"key":"e_1_3_4_3_2","unstructured":"Robert Koch-Institut. 2024. Influenza (Teil 1): erkrankungen durch saisonale influenzaviren. Available from: https:\/\/www.rki.de\/DE\/Content\/Infekt\/EpidBull\/Merkblaetter\/Ratgeber_Influenza_saisonal.html. Retrieved 12 Apr 2024."},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.05.020416"},{"key":"e_1_3_4_5_2","unstructured":"Robert Koch-Institut. 2024. RSV-Infektionen. Available from: https:\/\/www.rki.de\/DE\/Content\/Infekt\/EpidBull\/Merkblaetter\/Ratgeber_RSV.html. Retrieved 12 Apr 2024."},{"key":"e_1_3_4_6_2","unstructured":"World Health Organization. 2024. WHO COVID-19 dashboard. Available from: https:\/\/covid19.who.int. Retrieved 12 Dec 2023."},{"key":"e_1_3_4_7_2","unstructured":"Robert Koch-Institut. 2023. Epidemiologischer steckbrief zu SARS-CoV-2 und COVID-19. Available from: https:\/\/www.rki.de\/DE\/Content\/InfAZ\/N\/Neuartiges_Coronavirus\/Steckbrief.html?nn=13490888#doc13776792bodyText15. Retrieved 12 Dec 2023."},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01910.x"},{"key":"e_1_3_4_9_2","unstructured":"Arifin W. 2023. Sample size calculator. Available from: http:\/\/wnarifin.github.io. Retrieved 01 Oct 2023."},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01914-20"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3205\/lab000044"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.9.6.1235-1239.2002"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.12.009"},{"key":"e_1_3_4_14_2","unstructured":"Robert Koch-Institut. 2024. Arbeitsgemeinschaft Influenza. Available from: https:\/\/influenza.rki.de\/Diagrams.aspx?agiRegion=0. Retrieved 10 May 2024."},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD013705"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01611-19"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-03860-5"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2022.07.022"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01153-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01153-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T13:14:15Z","timestamp":1722950055000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01153-24"}},"subtitle":[],"editor":[{"given":"Melissa R.","family":"Gitman","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,8,6]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8,6]]}},"alternative-id":["10.1128\/spectrum.01153-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01153-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,6]]},"assertion":[{"value":"2024-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01153-24"}}