{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:58:52Z","timestamp":1793642332302,"version":"4.5.1"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/08224-9"],"award-info":[{"award-number":["2020\/08224-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/15578-4"],"award-info":[{"award-number":["2019\/15578-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"MCTI | Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["422984\/2021-3"],"award-info":[{"award-number":["422984\/2021-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"MCTI | Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["314336\/2021-4"],"award-info":[{"award-number":["314336\/2021-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["2020-72210089"],"award-info":[{"award-number":["2020-72210089"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,10,26]]},"abstract":"<jats:p>\n            Alerts regarding the emergence and increase of combinations of carbapenemases in\n            <jats:italic>Enterobacterales<\/jats:italic>\n            in Latin America and the Caribbean have recently been issued by PAHO and WHO, emphasizing the importance of appropriate microbiological diagnosis and the effective and articulated implementation of infection prevention and control programs. In this study, we evaluated methods based on inhibition of ceftazidime (CAZ), ceftazidime-avibactam (CZA), and aztreonam (ATM) by dipicolinic acid (DPA), EDTA, and avibactam (AVI) inhibitors for the identification of KPC-2- and NDM-1-coexpression in members of the\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            complex recovered from human and animal hosts.\n          <\/jats:p>","DOI":"10.1128\/spectrum.01159-22","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T13:01:31Z","timestamp":1660827691000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9447-8325","authenticated-orcid":false,"given":"Felipe","family":"V\u00e1squez-Ponce","sequence":"first","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Dantas","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johana","family":"Becerra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Melocco","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keila","family":"Lima","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline V.","family":"de Lima","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio P.","family":"Sellera","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, School of Veterinary Medicine and Animal Science, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"School of Veterinary Medicine, Metropolitan University of Santos, Santos, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Mattos","sequence":"additional","affiliation":[{"name":"Laborclin, Pinhais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Trevisoli","sequence":"additional","affiliation":[{"name":"Laborclin, Pinhais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco A.","family":"Vianello","sequence":"additional","affiliation":[{"name":"Natal Garrison Hospital, Brazilian Army, Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais","family":"Sincero","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, Health Sciences Center, Federal University of Santa Catarina, Florian\u00f3polis, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Di Conza","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Instituto de Investigaciones en Bacteriologia y Virolog\u00eda Molecular, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliana","family":"Vespero","sequence":"additional","affiliation":[{"name":"Department of Pathology, Clinical and Toxicological Analysis, Health Sciences Center, University Hospital of Londrina, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1999-9785","authenticated-orcid":true,"given":"Gabriel","family":"Gutkind","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Instituto de Investigaciones en Bacteriologia y Virolog\u00eda Molecular, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"Fleury Medicine and Health, Microbiology Section, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0161-5800","authenticated-orcid":true,"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"},{"name":"Department of Clinical Analysis, School of Pharmacy, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1813023"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2020.09.015"},{"key":"e_1_3_3_4_2","unstructured":"Pan American Health Organization. 2021. Epidemiological alert: emergence and increase of new combinations of carbapenemases in Enterobacterales in Latin America and the Caribbean 22 October 2021. PAHO\/WHO Washington D.C. https:\/\/www.paho.org\/en\/documents\/epidemiological-alert-emergence-and-increase-new-combinations-carbapenemases."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01080-21"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2014.0171"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02400-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06256-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239308"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02339-w"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00407"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2011.23.5.263"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00846-21"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00901-13"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001202"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12678"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2014\/7027.4614"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1179\/1973947815Y.0000000063"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02102-15"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-021-00464-5"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.5578\/mb.20229804"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11030373"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2022.01.025"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10111341"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10081012"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa326"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.0c00083"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-013-0013-7"},{"key":"e_1_3_3_29_2","unstructured":"Programa Nacional de Control de Calidad en Bacteriologia Inei Anlis Dr. Carlos G. Malbran. 2021. Alerta epidemiologica: emergencia de Enterobacterales doble productores de carbapenemasas. http:\/\/antimicrobianos.com.ar\/ATB\/wp-content\/uploads\/2021\/05\/Alerta-epidemiol\u00f3gica-dobles-productores-de-carbapenemasa-COVID-19-v4.pdf."},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00890-21"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz209"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01111-19"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-019-01326-1"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2021-036"},{"key":"e_1_3_3_35_2","unstructured":"World Health Organization. 2021. WHO integrated global surveillance on ESBL-producing E. coli using a \u201cOne Health\u201d approach: implementation and opportunities. https:\/\/apps.who.int\/iris\/rest\/bitstreams\/1335472\/retrieve."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.12.009"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.003"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-8216"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S357396"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.04.023"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz1155"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01008-17"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01659-20"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.868705"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2022.04.014"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2022.04.006"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-017-3035-3"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2021.10.009"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz149"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2020.11.004"},{"key":"e_1_3_3_51_2","unstructured":"European Committee on Antimicrobial Susceptibility Testing. 2022. Breakpoint tables for interpretation of MICs and zone diameters version 12.0. Accessed 1 January 2022. http:\/\/www.eucast.org\/clinical_breakpoints\/."},{"key":"e_1_3_3_52_2","unstructured":"Clinical and Laboratory Standards Institute. 2022. Performance standards for antimicrobial susceptibility testing. CLSI supplement M100 32nd ed. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000023410"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.12.002"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-02010-3"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.05.014"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5648-5652.2005"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.08.011"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01159-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01159-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T13:55:23Z","timestamp":1666792523000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01159-22"}},"subtitle":[],"editor":[{"given":"Maria Antonia","family":"De Francesco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,10,26]]},"references-count":57,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,10,26]]}},"alternative-id":["10.1128\/spectrum.01159-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01159-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,26]]},"assertion":[{"value":"2022-04-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01159-22"}}