{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T07:04:22Z","timestamp":1774508662878,"version":"3.50.1"},"reference-count":100,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"EC | HORIZON EUROPE Framework Programme","award":["874735 (VEO)"],"award-info":[{"award-number":["874735 (VEO)"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The microbial communities of disease vectors may represent a key feature in several biological functions and thus deserve special attention in light of climate change and the consequent need to develop novel control strategies. Nevertheless, vector-borne microbial networks are still poorly understood. Assessing vectors\u2019 microbial interactions and climatic dependencies may contribute to better estimating pathogen transmission characteristics and public health risks. In a climatically representative country-wide survey,\n            <jats:italic>Ixodes ricinus<\/jats:italic>\n            ticks were collected from 17 locations in Hungary. Using shotgun metagenomic sequencing, the bacteriome composition was analyzed by investigating the relationship between the abundance of nymphs and females in various climatic environments. Bacterial composition on the genus level revealed a significant difference between the samples from females and nymphs. Within the core bacteriome, females and nymphs showed significant variation in the following genera:\n            <jats:italic>Arsenophonus<\/jats:italic>\n            ,\n            <jats:italic>Bacillus<\/jats:italic>\n            ,\n            <jats:italic>Candidatus Midichloria<\/jats:italic>\n            ,\n            <jats:italic>Rhodococcus<\/jats:italic>\n            ,\n            <jats:italic>Sphingomonas<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus<\/jats:italic>\n            , and\n            <jats:italic>Wolbachia<\/jats:italic>\n            . Among females, according to temperature strata, the following were found differentiating:\n            <jats:italic>Curtobacterium<\/jats:italic>\n            ,\n            <jats:italic>Pseudomonas<\/jats:italic>\n            , and\n            <jats:italic>Sphingomonas<\/jats:italic>\n            . There was no genus with a significant difference in precipitation categories for females.\n            <jats:italic>Curtobacterium<\/jats:italic>\n            showed significant variation between temperature and\n            <jats:italic>Bacillus<\/jats:italic>\n            and\n            <jats:italic>Curtobacterium<\/jats:italic>\n            for various precipitation levels in the nymphs. The composition of vector-borne bacteriome members showed significant alterations at sampling points with different climatic conditions and development stages of the tick hosts. Our findings not only pave the way toward understanding tick-borne bacterial networks and interdependencies but also shed light on the high potential for the presence of a possible biological tick control species, the tick parasitoid,\n            <jats:italic>Ixodiphagus hookeri<\/jats:italic>\n            based on related bacteriome patterns.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Climate-sensitive disease vectors, such as ticks, respond to the environment with changes in their microbiome. These changes can affect the emergence or re-emergence of various vector-borne pathogens, such as the causative agent of Lyme borreliosis (LB) or tick-borne encephalitis. This aspect is particularly emphasized in light of climate change. The climatically representative assessment of microbiome differences in various developmental stages of the most common Central European tick species,\n              <jats:italic>Ixodes ricinus<\/jats:italic>\n              , deepens our understanding of the potential climatic factors behind microbial relative abundance and interaction changes. This knowledge can support the development of novel disease vector control strategies.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01243-23","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T14:03:08Z","timestamp":1700056988000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["<i>Ixodes ricinus<\/i>\n            tick bacteriome alterations based on a climatically representative survey in Hungary"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9291-9759","authenticated-orcid":false,"given":"Adrienn Gr\u00e9ta","family":"T\u00f3th","sequence":"first","affiliation":[{"name":"Centre for Bioinformatics, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2064-0147","authenticated-orcid":false,"given":"R\u00f3bert","family":"Farkas","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Zoology, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4975-253X","authenticated-orcid":false,"given":"M\u00e1rton","family":"Papp","sequence":"additional","affiliation":[{"name":"Centre for Bioinformatics, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3457-2739","authenticated-orcid":false,"given":"Oz","family":"Kilim","sequence":"additional","affiliation":[{"name":"Department of Physics of Complex Systems, E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haeun","family":"Yun","sequence":"additional","affiliation":[{"name":"Centre for Bioinformatics, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7273-9133","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Makrai","sequence":"additional","affiliation":[{"name":"Autovakcina Ltd , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3705-0461","authenticated-orcid":false,"given":"Gergely","family":"Mar\u00f3ti","sequence":"additional","affiliation":[{"name":"Institute of Plant Biology, Biological Research Center, HUN-REN , Szeged, Hungary"},{"name":"Faculty of Water Sciences , University of Public Service, Baja, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nika","family":"Gyurkovszky","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Zoology, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"Krik\u00f3","sequence":"additional","affiliation":[{"name":"Centre for Bioinformatics, University of Veterinary Medicine , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1783-2041","authenticated-orcid":true,"given":"Norbert","family":"Solymosi","sequence":"additional","affiliation":[{"name":"Centre for Bioinformatics, University of Veterinary Medicine , Budapest, Hungary"},{"name":"Department of Physics of Complex Systems, E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/321314"},{"key":"e_1_3_4_3_2","unstructured":"World health organization\n. 2017. Global vector control response 2017-2030"},{"key":"e_1_3_4_4_2","first-page":"1","article-title":"Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change","volume":"5","author":"Stocker T","year":"2013","unstructured":"Stocker T , Qin D , Plattner G , Tignor M , Allen S , Boschung J . 2013. Contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change. Climate change 5:1\u20131552. https:\/\/www.ipcc.ch\/site\/assets\/uploads\/2018\/02\/WG1AR5_all_final.pdf.","journal-title":"Climate change"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx244"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.04.015"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000213069"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.16.27.19906-en"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-8"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-6-1"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01377-22"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-019-00461-6"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2015.03.010"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01051-8"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1079\/9781789249637.0000"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0273844"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/qj.828"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1198\/016214504000000250"},{"key":"e_1_3_4_20_2","unstructured":"R Core Team\n. 2022. R: a language and environment for statistical computing. Vienna Austria. Available from: https:\/\/www.R-project.org"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt593"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1891-0"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.18129\/B9.bioc.microbiome"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005404"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv033"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1290"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8228-8235.2005"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x"},{"key":"e_1_3_4_31_2","unstructured":"Oksanen J Blanchet FG Friendly M Kindt R Legendre P McGlinn D\n. 2019. Vegan: community ecology package. Available from: https:\/\/CRAN.R-project.org\/package=vegan"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-017-0237-y"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002687"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004226"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0213384"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025604"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2021.101768"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00053311"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2017.11.004"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1969"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.110308.120206"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.208.4448.1157"},{"key":"e_1_3_4_44_2","volume-title":"Evolutionary Ecology of parasites","author":"Poulin R","year":"2011","unstructured":"Poulin R . 2011. Evolutionary ecology of parasites. In Evolutionary Ecology of parasites. Princeton university press."},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9050339"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-28969-3"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2022.102053"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183543"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2574-x"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2708.204763"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050378"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2008153244"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3666-3671.2001"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.15654\/TPK-170065"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3240-7"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1613422114"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-013-9762-y"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14094"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1808.110434"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-11"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1894-6"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.10424"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.719-756.2005"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000047"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2352-9"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.719"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-019-1514-7"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.15414\/jmbfs.2019.8.6.1340-1342"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-04740-3"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00236"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13832"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2021.2781"},{"key":"e_1_3_4_73_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149950"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007001"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-143"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/35.6.1050"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0418.2010.01560.x"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2010.12.004"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.67.1735.351"},{"key":"e_1_3_4_80_2","first-page":"108","article-title":"The utilization of Hunterellus hookeri How. for the control of the ticks, Ixodes ricinus L. and Ixodes persulcatus P. Sch. with reference to peculiarities of their metamorphosis under conditions of the Province of Lenningrad","volume":"34","author":"Alfeev N","year":"1946","unstructured":"Alfeev N . 1946. The utilization of Hunterellus hookeri How. for the control of the ticks, Ixodes ricinus L. and Ixodes persulcatus P. Sch. with reference to peculiarities of their metamorphosis under conditions of the Province of Lenningrad. Review of Applied Entomology 34:108\u2013109.","journal-title":"Review of Applied Entomology"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-228"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030692"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005048"},{"key":"e_1_3_4_84_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-019-01469-6"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2006.01024.x"},{"key":"e_1_3_4_86_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00248-08"},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2003.08.004"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-4017(01)00650-1"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0556"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2008.00468.x"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1673\/031.010.14146"},{"key":"e_1_3_4_92_2","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evy211"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0032-2018"},{"key":"e_1_3_4_94_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-6096-z"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00008131"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12926"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00340-11"},{"key":"e_1_3_4_98_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463201002300120"},{"key":"e_1_3_4_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01584-20"},{"key":"e_1_3_4_100_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2013.01.005"},{"key":"e_1_3_4_101_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.4975-4983.2001"},{"key":"e_1_3_4_102_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.113"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01243-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01243-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T13:59:46Z","timestamp":1702389586000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01243-23"}},"subtitle":[],"editor":[{"given":"Anna","family":"Moniuszko-Malinowska","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,12,12]]},"references-count":100,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,12,12]]}},"alternative-id":["10.1128\/spectrum.01243-23"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01243-23","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,12]]},"assertion":[{"value":"2023-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01243-23"}}