{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:24:14Z","timestamp":1788269054640,"version":"build-2803163510"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000738","name":"US Department of Veterans Affairs","doi-asserted-by":"publisher","award":["VASEQCURE"],"award-info":[{"award-number":["VASEQCURE"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Carbapenem resistance is a serious threat to public health because carbapenems are used as last-resort antibiotics. Carbapenem resistance gene KPC (\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      carbapenemase) inactivates a broad range of \u03b2-lactam substrates. In this manuscript, we examined intra-host transmission of\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      KPC-3 via interspecies gene transfer. Two carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      isolates and one\n                      <jats:italic toggle=\"yes\">Klebsiella michiganensis<\/jats:italic>\n                      isolate were identified from two patients. Genetic relations of these isolates were investigated with whole-genome sequencing (WGS). Hybrid assembly of bacterial genomes showed the three isolates carried plasmids that harbor common antimicrobial resistance (AMR) gene clusters that confer multidrug-class resistance, including carbapenems. Our results suggest that AMR gene clusters are disseminated across the species as fragments rather than as complete, intact plasmids.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        An antimicrobial resistance gene cluster encompassing multiple drug classes on plasmids could lead a drug-susceptible pathogen to gain multidrug resistance. Interspecies gene transfer enables\n                        <jats:italic toggle=\"yes\">K. michiganensis<\/jats:italic>\n                        to become multidrug-resistant through the acquisition of clustered, plasmid-encoded resistance genes spanning multiple antibiotic classes.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01254-26","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:00:16Z","timestamp":1786626016000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Plasmid-mediated dissemination of\n                    <i>bla<\/i>\n                    KPC-3 and multidrug resistance genes among different species of\n                    <i>Klebsiella<\/i>"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8272-9828","authenticated-orcid":true,"given":"Hosoon","family":"Choi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02dcp1550","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Research, Central Texas Veterans Health Care System","place":["Temple, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6300-2280","authenticated-orcid":true,"given":"Munok","family":"Hwang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02dcp1550","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Research, Central Texas Veterans Health Care System","place":["Temple, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0070-5919","authenticated-orcid":true,"given":"Dhammika H.","family":"Navarathna","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02dcp1550","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine Services, Central Texas Veterans Health Care System","place":["Temple, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3916-952X","authenticated-orcid":true,"given":"Chetan","family":"Jinadatha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02dcp1550","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Central Texas Veterans Health Care System","place":["Temple, USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, College of Medicine, Texas A&M University","place":["Bryan, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-07"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03553.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2022.2152484"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1151-1161.2001"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4793-4799.2004"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00693-09"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-95285-z"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2019.100516"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04235-22"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114351"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-012-0245-x"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2021.51"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02749-13"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3290"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3962"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/ismejo\/wrad032"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"Curtis","family":"Donskey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Majie C.","family":"Foster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01254-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01254-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:11:56Z","timestamp":1788268316000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01254-26"}},"subtitle":[],"editor":[{"given":"Krisztina 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