{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:26:27Z","timestamp":1791851187745,"version":"4.3.4"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100013314","name":"111 Project","doi-asserted-by":"crossref","award":["D20009"],"award-info":[{"award-number":["D20009"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32060034, 32360048"],"award-info":[{"award-number":["32060034, 32360048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China-Ukraine Intergovermental Exchange Project","award":["8"],"award-info":[{"award-number":["8"]}]},{"name":"International Science and Technology Cooperation Base of Guizhou Province","award":["[2020]4101"],"award-info":[{"award-number":["[2020]4101"]}]},{"name":"Talent Base Project of Guizhou Province, China","award":["RCJD2018-22"],"award-info":[{"award-number":["RCJD2018-22"]}]},{"name":"High-level Innovation Talent Project of Guizhou Province","award":["GCC[2022]036e1"],"award-info":[{"award-number":["GCC[2022]036e1"]}]},{"name":"Foundation of Key Laboratory of Microbiology and Parasitology of Education Department, Guizhou","award":["QJJ [2022] 019"],"award-info":[{"award-number":["QJJ [2022] 019"]}]},{"name":"Ministry of Education Projects","award":["07150120711"],"award-info":[{"award-number":["07150120711"]}]},{"name":"Guizhou Province Science and Technology Plan project","award":["ZK [2023] generally 332"],"award-info":[{"award-number":["ZK [2023] generally 332"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Backed by advancements in technologies like microbial sequencing, many studies indicate that the vaginal microbiome is a key marker of female reproductive health. However, further studies are still needed to investigate the correlation between vaginal microbiota (VMB) and outcomes of assisted reproductive technology (ART). Therefore, this study compared the VMB of two types of infertile women undergoing\n              <jats:italic>in vitro<\/jats:italic>\n              fertilization (IVF) with normal control women during the implantation window period and investigated the effects of VMB characteristics on IVF outcomes. Vaginal swabs from IVF patients (\n              <jats:italic>n<\/jats:italic>\n              = 85) were collected before embryo transfer (ET), and vaginal swabs from normal control women (\n              <jats:italic>n<\/jats:italic>\n              = 37) were collected during the 6\u20138 days of ovulation detection. Results showed differences in the vaginal community structure between infertile women with polycystic ovary syndrome (PCOS) and with tubal factor infertility (TFI) undergoing IVF treatment. The results revealed a higher relative abundance of\n              <jats:italic>Lactobacillus iners<\/jats:italic>\n              in the non-pregnant group compared with the pregnant group. The results also demonstrated the abundance of\n              <jats:italic>Pseudomonas<\/jats:italic>\n              spp. in both non-pregnant groups of infertile women. The findings suggested that the VMB composition affects the IVF outcomes and that the pre-ET high abundance of\n              <jats:italic>L. iners<\/jats:italic>\n              may potentially indicate an IVF failure. The abundance of\n              <jats:italic>Pseudomonas<\/jats:italic>\n              spp. in the vagina may be an adverse factor for ART pregnancy.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Many studies suggest that vaginal microbiota (VMB) may affect\n              <jats:italic>in vitro<\/jats:italic>\n              fertilization-embryo transfer (IVF-ET) outcomes. Assessing VMB before embryo transfer can optimize timing for better assisted reproductive technology (ART) results. This study examined VMB distribution in infertile women undergoing ART using 16S rRNA sequencing. Results revealed that VMB structure impacted ART outcomes in women with polycystic ovary syndrome (PCOS) and tubal factor infertility (TFI) before embryo transfer ([less than or equal to] 24 hours).\n              <jats:italic>Lactobacillus iners<\/jats:italic>\n              and\n              <jats:italic>Pseudomonas<\/jats:italic>\n              spp. were identified as adverse factors for post-ET pregnancy. The study also showed differences in pre-ET VMB between normal women and women with PCOS and TFI during the ovulatory window. These findings highlight the importance of considering VMB composition to optimize embryo transfer timing and personalize ART treatment based on infertility type, improving the chances of success.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01255-24","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T14:00:53Z","timestamp":1737986453000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Effects of vaginal microbiota on\n            <i>in vitro<\/i>\n            fertilization outcomes in women with different infertility causes"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7617-461X","authenticated-orcid":false,"given":"Huimin","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University","place":["Guiyang, China"]},{"name":"Department of Microbiology, Basic Medical College, Guizhou Medical University","place":["Guiyang, China"]},{"name":"Guizhou Talent Base for Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou","place":["Guiyang, China"]},{"name":"Medical Affairs Department, National Regional Medical Center, Sir Run Run Shaw Hospital, Alaer Hospital, Zhejiang University School of Medicine","place":["Alaer, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Basic Medical College, Guizhou Medical University","place":["Guiyang, China"]},{"name":"Guizhou Talent Base for Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou","place":["Guiyang, China"]},{"name":"Guiyang Maternal and Child Health Hospital, Reproductive Medicine Center","place":["Guiyang, 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