{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:04:01Z","timestamp":1791648241527,"version":"4.3.4"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Infective endocarditis (IE) is a major life-threatening disease. However, the inability to identify the causative pathogen using conventional microbiological cultures jeopardizes the choice of optimal antibiotic therapy. Culture-independent molecular techniques for pathogen detection may overcome this challenge. Here, two molecular tests (16S\/18S rDNA assay [SepsiTest, Molzym, Germany]; Biofire Joint Infection Panel [BJP, bioM\u00e9rieux, Marcy l\u2019\u00c9toile, France]) were analyzed for their ability to detect pathogens in a series of 100 explanted heart valves. In total, at least one pathogen was detected in 67\/100 specimens by one or more methods, and 33\/100 specimens were negative in all three assays. Overall, valve-culture had an inferior sensitivity (39.4%), compared to 16S\/18S rDNA PCR (90.9%) and the BJP (83.1% [all organisms], 98.2% [on-panel organisms only]). In culture-negative cases, 35 and 32 additional pathogens were identified by 16S\/18S rDNA PCR and BJP, respectively. In five culture- and 16S\/18S rDNA assay-negative cases, BJP identified correctly additional pathogens. False-positive results were observed in one (16S\/18S rDNA PCR) and two (BJP) cases, respectively. In conclusion, both culture-independent assays proved to be reliable and increased the diagnostic yield compared to culture. In general, false negatives (i.e., off-panel pathogens in BJP) and false positives require careful clinical evaluation of results from culture-independent techniques. Diagnostic stewardship is essential to guide the optimal use of molecular techniques in the work-up of infective endocarditis.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Infective endocarditis (IE) therapy must be guided by the identification of the causative pathogen. Microbiological cultures, however, remain negative in a significant number of cases, jeopardizing the choice of optimal anti-infective treatment. This study evaluated the ability of two independent molecular tests (16\/18S rDNA PCR, syndromic panel PCR [Biofire JI panel]) to overcome limitations of conventional microbiological approaches to detect microorganisms from explanted heart valve tissues. Both assays proved to have a greater sensitivity compared to conventional culture, and PCR and BJP identified a pathogen in 35 and 32 out of 74 culture negative IE cases, additional pathogens, respectively. The study highlights the great importance of culture-independent approaches to pathogen detection in IE and guides the choice of the optimal assay using diagnostic stewardship interventions.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01263-25","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T14:17:58Z","timestamp":1753193878000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Comparative evaluation of Biofire Joint Infection Panel, Sepsitest 16S\/18S rDNA PCR, and culture to identify microorganisms in explanted heart valves"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2749-1201","authenticated-orcid":false,"given":"Benjamin","family":"Berinson","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Tanida","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Wolters","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flaminia","family":"Olearo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Sinning","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Department for Cardiology, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Christner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Aepfelbacher","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8587-4433","authenticated-orcid":false,"given":"Holger","family":"Rohde","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurjpc\/zwab211"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehad193"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00635-17"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad271"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/653675"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000165658.82869.17"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15266-X"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.124.040218"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2014.07.028"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11081372"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-021-01690-x"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00341-23"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0113-7"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2023.115926"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066183"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100956"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00543-21"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00592-23"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1302\/2058-5241.6.200118"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01126-22"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-022-04538-w"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02239-24"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01575-18"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000804"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.10.082"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1107170"},{"key":"e_1_3_5_28_2","unstructured":"Tanida K et al.. 2025. Evaluation of a commercial 16S rDNA PCR assay (UMD-SelectNA Molzym) for pathogen detection from cardiac tissue specimens. medRxiv"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01263-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01263-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T13:04:52Z","timestamp":1756818292000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01263-25"}},"subtitle":[],"editor":[{"given":"Paul M.","family":"Luethy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":27,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,2]]}},"alternative-id":["10.1128\/spectrum.01263-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01263-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-04-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01263-25"}}