{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:13:34Z","timestamp":1788225214283,"version":"build-2803163510"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Ministry of Science and Technology, Taiwan","award":["109-2320-B-010-036-MY3"],"award-info":[{"award-number":["109-2320-B-010-036-MY3"]}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"crossref","award":["CMRPG3L1581"],"award-info":[{"award-number":["CMRPG3L1581"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"crossref","award":["CMRPG3N0091"],"award-info":[{"award-number":["CMRPG3N0091"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,10,17]]},"abstract":"ABSTRACT<\/jats:title>\n \n Lugdunin produced by\n Staphylococcus lugdunensis<\/jats:italic>\n has been shown to have broad inhibitory activity against Gram-positive bacteria; however, lugdunin activity among\n S. lugdunensis<\/jats:italic>\n isolates and its association with different\n agr<\/jats:italic>\n , SCC\n mec<\/jats:italic>\n , and sequence types remain unclear. We used matrix-assisted laser desorption ionization-time-of-flight mass spectrometry to identify\n S. lugdunensis<\/jats:italic>\n and collected 202\n S<\/jats:italic>\n .\n lugdunensis<\/jats:italic>\n samples for further assays. Agar spot tests were performed to characterize\n S. lugdunensis<\/jats:italic>\n lugdunin production and activity. Multilocus sequence typing, SCC\n mec,<\/jats:italic>\n and\n agr<\/jats:italic>\n genotyping were performed on\n S. lugdunensis<\/jats:italic>\n . In all, 91 S\n taphylococcus aureus<\/jats:italic>\n strains with varying vancomycin susceptibilities were used to examine lugdunin activity in\n S. lugdunensis<\/jats:italic>\n . In total, 48\n S<\/jats:italic>\n .\n lugdunensis<\/jats:italic>\n strains (23.8%) were found to be oxacillin-resistant\n S. lugdunensis<\/jats:italic>\n (ORSL), whereas 154 (76.2%) were classified as oxacillin-sensitive\n S. lugdunensis<\/jats:italic>\n (OSSL). Moreover, 16 (33.3%) ORSL and 35 (22.7%) OSSL strains showed antibacterial activity against\n S. aureus<\/jats:italic>\n . Our data showed that most lugdunin-producing ORSL strains (14\/48, 29.2%) were of ST3-SCC\n mec<\/jats:italic>\n V-\n agr<\/jats:italic>\n II genotypes, whereas most lugdunin-producing OSSL strains (15\/154, 9.7%) were of ST3-\n agr<\/jats:italic>\n II, followed by ST1-\n agr<\/jats:italic>\n I (10\/154, 6.5%). Our data also revealed that lugdunin exhibited weak inhibitory activity against the VISA ST239 isolate. In addition, we observed that ST239 VSSA was more resistant to lugdunin than ST5, ST59, and ST45 VSSA. Taken together, our data pioneered the epidemiology of lugdunin production in\n S. lugdunensis<\/jats:italic>\n isolates and revealed its association with genotypes. However, further molecular and bioinformatics investigations are needed to elucidate the regulatory mechanisms of lugdunin production and activity.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Lugdunin is active against both methicillin-resistant\n Staphylococcus aureus<\/jats:italic>\n and vancomycin-resistant enterococci by dissipating their membrane potential. However, the association of lugdunin activity with the genotypes of\n Staphylococcus lugdunensis<\/jats:italic>\n has not been addressed. Here, we show the high prevalence of lugdunin-producing strains among ST1 (83.3%), ST2 (66.7%), and ST3 (53.3%)\n S. lugdunensis<\/jats:italic>\n . Moreover, we identified the antibacterial activity of lugdunin-producing strains against VISA and hVISA. These results shed light on the potential application of lugdunin for the treatment of drug-resistant pathogens.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.01298-23","type":"journal-article","created":{"date-parts":[[2023,9,21]],"date-time":"2023-09-21T09:06:29Z","timestamp":1695287189000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Lugdunin production and activity in\n Staphylococcus lugdunensis<\/i>\n isolates are associated with its genotypes"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5921-7974","authenticated-orcid":false,"given":"Shih-Cheng","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital","place":["Taoyuan, Taiwan"]},{"name":"Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University","place":["Taoyuan, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Yen","family":"Kao","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University","place":["Taipei, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee-Chung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital","place":["Taoyuan, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jazon Harl","family":"Hidrosollo","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University","place":["Taipei, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang-Jih","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital","place":["Taoyuan, Taiwan"]},{"name":"Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University","place":["Taoyuan, Taiwan"]},{"name":"Department of Medicine, College of Medicine, Chang Gung University","place":["Taoyuan, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2020.06.009"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00461"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.05.013"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61999-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0029-2018"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00205-20"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02813.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00846-17"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature19781"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00988-12"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2015.05.022"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2021.02.009"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01247-21"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2022.08.021"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11010086"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-06307-0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01598-21"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00099-22"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.4.399"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2014.12.006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01734-20"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.765437"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00197-22"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00165-06"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134859"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20442"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-4978-1"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00528-21"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201901589"}],"container-title":["Microbiology 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