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Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize five coagulase-negative strains from the\n                    <jats:italic toggle=\"yes\">Staphylococcus haemolyticus<\/jats:italic>\n                    phylogenetic clade obtained from human ear swabs, wounds, and bile. Based on the results of polyphasic taxonomy, we propose the species\n                    <jats:italic toggle=\"yes\">Staphylococcus brunensis<\/jats:italic>\n                    sp. nov. (type strain NRL\/St 16\/872\n                    <jats:sup>T<\/jats:sup>\n                    = CCM 9024\n                    <jats:sup>T<\/jats:sup>\n                    = LMG 31872\n                    <jats:sup>T<\/jats:sup>\n                    = DSM 111349\n                    <jats:sup>T<\/jats:sup>\n                    ). The genomic analysis revealed numerous variable genomic elements, including staphylococcal cassette chromosome (SCC), prophages, plasmids, and a unique 18.8 kb-long genomic island SbCI\n                    <jats:italic toggle=\"yes\">\n                      <jats:sub>ccrDE<\/jats:sub>\n                    <\/jats:italic>\n                    integrated into the ribosomal protein L7 serine acetyltransferase gene\n                    <jats:italic toggle=\"yes\">rimL<\/jats:italic>\n                    . SbCI\n                    <jats:italic toggle=\"yes\">\n                      <jats:sub>ccrDE<\/jats:sub>\n                    <\/jats:italic>\n                    has a cassette chromosome recombinase (\n                    <jats:italic toggle=\"yes\">ccr<\/jats:italic>\n                    ) gene complex with a typical structure found in SCCs. Based on nucleotide and amino acid identity to other known\n                    <jats:italic toggle=\"yes\">ccr<\/jats:italic>\n                    genes and the distinct integration site that differs from the canonical methyltransferase gene\n                    <jats:italic toggle=\"yes\">rlmH<\/jats:italic>\n                    exploited by SCCs, we classified the\n                    <jats:italic toggle=\"yes\">ccr<\/jats:italic>\n                    genes as novel variants,\n                    <jats:italic toggle=\"yes\">ccrDE<\/jats:italic>\n                    . The comparative genomic analysis of SbCI\n                    <jats:italic toggle=\"yes\">\n                      <jats:sub>ccrDE<\/jats:sub>\n                    <\/jats:italic>\n                    with related islands shows that they can accumulate virulence and antimicrobial resistance factors creating novel resistance elements, which reflects the evolution of SCC. The spread of these resistance islands into established pathogens such as\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    would pose a great threat to the healthcare system.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The coagulase-negative staphylococci are important opportunistic human pathogens, which cause bloodstream and foreign body infections, mainly in immunocompromised patients. The mobile elements, primarily the staphylococcal cassette chromosome\n                      <jats:italic toggle=\"yes\">mec<\/jats:italic>\n                      , which confers resistance to methicillin, are the key to the successful dissemination of staphylococci into healthcare and community settings. Here, we present a novel species of the\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      genus isolated from human clinical material. The detailed analysis of its genome revealed a previously undescribed genomic island, which is closely related to the staphylococcal cassette chromosome and has the potential to accumulate and spread virulence and resistance determinants. The island harbors a set of conserved genes required for its mobilization, which we recognized as novel cassette chromosome recombinase genes\n                      <jats:italic toggle=\"yes\">ccrDE<\/jats:italic>\n                      . Similar islands were revealed not only in the genomes of coagulase-negative staphylococci but also in\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      . The comparative genomic study contributes substantially to the understanding of the evolution and pathogenesis of staphylococci.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01342-23","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T09:00:25Z","timestamp":1694768425000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>Staphylococcus brunensis<\/i>\n                    sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the\n                    <i>rlmH<\/i>\n                    gene bearing the\n                    <i>ccrDE<\/i>\n                    recombinase gene complex"],"prefix":"10.1128","volume":"11","author":[{"given":"Vojt\u011bch","family":"Kova\u0159ovic","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, 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