{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:46:23Z","timestamp":1785415583385,"version":"3.56.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2301801"],"award-info":[{"award-number":["2021YFC2301801"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272313"],"award-info":[{"award-number":["82272313"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022MH006"],"award-info":[{"award-number":["ZR2022MH006"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The identification of predictive indices for hepatitis B e antigen seroconversion (HBeAg SC) in patients with chronic hepatitis B (CHB) remains a challenge. We aimed to investigate whether the TUBB1 promoter methylation in peripheral blood mononuclear cells (PBMCs) can predict HBeAg SC. A total of 271 participants were recruited, comprising 145 patients with HBeAg-positive CHB, 94 with HBeAg-negative CHB, and 32 healthy controls (HCs). The patients with HBeAg-positive CHB were followed up for 72 weeks. The TUBB1 promoter methylation and the corresponding mRNA levels in PBMCs were detected using MethyLight and quantitative real-time PCR, respectively. The methylation levels of the TUBB1 promoter were remarkably elevated in patients with positive HBeAg, in comparison to those with negative HBeAg and HCs. Conversely, the relative mRNA expression levels of TUBB1 were significantly downregulated in patients with positive HBeAg, when compared to those with negative HBeAg and HCs. The TUBB1 promoter methylation levels showed a gradual decrease across the four phases of CHB. Patients with HBeAg SC had lower baseline methylation levels of the TUBB1 promoter than those without HBeAg SC. The TUBB1 promoter methylation was an independent predictor of HBeAg SC (odds ratio [OR] = 0.683, 95% CI 0.553\u20130.845,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). The methylation of the TUBB1 promoter showed good predictive value for HBeAg SC in patients with positive HBeAg (area under the curve [AUC] = 0.805, 95% CI 0.704\u20130.907,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). The methylation level of the TUBB1 promoter might be a potent biomarker for predicting HBeAg SC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Previous studies emphasized hepatitis B e antigen seroconversion (HBeAg SC) as a milestone for chronic hepatitis B (CHB) remission associated with reduced disease progression risks. While the significance of HBeAg SC is widely recognized, reliable non-invasive predictors for achieving this endpoint remain limited. Additionally, in our previous studies, DNA methylation of key regulatory genes has been linked to CHB progression. However, the association between TUBB1 promoter methylation and HBeAg SC, as well as its potential as a biomarker for clinical application, has not been fully elucidated. We demonstrated that TUBB1 promoter methylation levels were significantly higher in HBeAg-positive patients and that decreased methylation levels were independently associated with subsequent HBeAg SC during a 72-week follow-up. Our findings underscore the potential clinical utility of TUBB1 promoter methylation as a non-invasive biomarker for predicting HBeAg SC. This study provides strong evidence supporting the role of TUBB1 promoter methylation in predicting HBeAg SC, offering a novel biomarker for monitoring CHB.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01344-25","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:04:15Z","timestamp":1760015055000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["TUBB1 promoter methylation is a promising biomarker for predicting HBeAg seroconversion in chronic hepatitis B"],"prefix":"10.1128","volume":"13","author":[{"given":"Tong","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuna","family":"Tang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihui","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Fan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]},{"id":[{"id":"https:\/\/ror.org\/0207yh398","id-type":"ROR","asserted-by":"publisher"}],"name":"Hepatology Institute of Shandong University, Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Cui","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Qilu Hospital of Shandong University Dezhou Hospital","place":["Dezhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8444-5415","authenticated-orcid":false,"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]},{"id":[{"id":"https:\/\/ror.org\/0207yh398","id-type":"ROR","asserted-by":"publisher"}],"name":"Hepatology Institute of Shandong University, Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6297-0147","authenticated-orcid":true,"given":"Kai","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056ef9489","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Hepatology, Qilu Hospital of Shandong University","place":["Jinan, China"]},{"id":[{"id":"https:\/\/ror.org\/0207yh398","id-type":"ROR","asserted-by":"publisher"}],"name":"Hepatology Institute of Shandong University, Shandong University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","volume-title":"Global hepatitis report 2024: action for access in low- and middle-income countries","author":"World Health Organization","year":"2024","unstructured":"World Health Organization. 2024. 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