{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:08:13Z","timestamp":1788570493095,"version":"build-2803163510"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["19K08964"],"award-info":[{"award-number":["19K08964"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japan Antibiotic Research Association","award":["Encouragement Award"],"award-info":[{"award-number":["Encouragement Award"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Reports of novel species of \u03b1-hemolytic\n              <jats:italic>Streptococcus<\/jats:italic>\n              have increased recently. However, limited information exists regarding the pathogenicity of these species, with the exception of\n              <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n              and\n              <jats:italic>Streptococcus pseudopneumoniae<\/jats:italic>\n              . In this study, a quinolone-resistant \u03b1-\n              <jats:italic>Streptococcus<\/jats:italic>\n              strain, MTG105, was isolated from the sputum of a patient with pneumonia. This strain was first identified as\n              <jats:italic>S. pneumoniae<\/jats:italic>\n              at the hospital laboratory; however, it exhibited unique genetic features upon further analysis. Digital DNA-DNA hybridization and average nucleotide identity based on BLAST values from whole-genome sequencing revealed MTG105 to be a novel species closely related to\n              <jats:italic>S. pseudopneumoniae<\/jats:italic>\n              . Although MTG105 carried two copies of the pneumolysin gene, similar to\n              <jats:italic>S. pseudopneumoniae<\/jats:italic>\n              , this isolate exhibited susceptibility to optochin under both aerobic and 5% CO\n              <jats:sub>2<\/jats:sub>\n              conditions. Notably, no biochemical features could be used to definitively identify this species. In an infection assay using organotypic lung tissue models, MTG105 induced epithelial damage comparable to that of\n              <jats:italic>S. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>S. pseudopneumoniae<\/jats:italic>\n              , possibly suggesting its potential as a pathogenic \u03b1-\n              <jats:italic>Streptococcus<\/jats:italic>\n              . The natural transformation abilities of\n              <jats:italic>Streptococcus<\/jats:italic>\n              species facilitate their exchange of genes within the same genus, resulting in the existence of species with increasingly more diverse genome structures. Therefore, the identification of this species highlights the importance of monitoring the emergence of novel species exhibiting virulence and\/or multidrug resistance. This isolate was proposed as a novel species, designated\n              <jats:italic>Streptococcus nakanoensis<\/jats:italic>\n              sp. nov. The type strain was MTG 105\n              <jats:sup>T<\/jats:sup>\n              (= JCM 35953\n              <jats:sup>T<\/jats:sup>\n              = CCUG 76894\n              <jats:sup>T<\/jats:sup>\n              ).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The genus\n              <jats:italic>Streptococcus<\/jats:italic>\n              encompasses a wide range of bacteria with more than 60 species. Recently, there has been a notable increase in reports of novel species of \u03b1-\n              <jats:italic>Streptococcus<\/jats:italic>\n              based on genomic analysis data. However, limited information exists regarding the pathogenicity of these species. In this study, a quinolone-resistant \u03b1-hemolytic\n              <jats:italic>Streptococcus<\/jats:italic>\n              strain, MTG105, was isolated from a patient with pneumonia. Genetic analysis revealed that this species was a novel species closely related to\n              <jats:italic>S. pseudopneumoniae<\/jats:italic>\n              . In an infection assay using organotypic lung tissue models, MTG105 induced epithelial damage comparable to that caused by\n              <jats:italic>S. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>S. pseudopneumoniae<\/jats:italic>\n              , strongly suggesting its potential as a pathogenic \u03b1-\n              <jats:italic>Streptococcus<\/jats:italic>\n              . The natural transformation abilities of\n              <jats:italic>Streptococcus<\/jats:italic>\n              species facilitate gene exchange within the same genus, leading to the emergence of species with increasingly diverse genome structures. Therefore, the identification of this species underscores the importance of monitoring the emergence of novel species exhibiting virulence and\/or multidrug resistance.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01354-24","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T13:12:48Z","timestamp":1726233168000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Molecular characterization of a novel putative pathogen,\n            <i>Streptococcus nakanoensis<\/i>\n            sp. nov., isolated from sputum culture"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9506-707X","authenticated-orcid":true,"given":"Takeaki","family":"Wajima","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Tokyo General Hospital, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0583-594X","authenticated-orcid":false,"given":"Emi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9337-6354","authenticated-orcid":true,"given":"Kei-ichi","family":"Uchiya","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.4.613-630.2002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-406"},{"key":"e_1_3_3_4_2","volume-title":"Manual of clinical microbiology","author":"Spellerberg B","year":"2007","unstructured":"Spellerberg B, Brandt C. 2007. 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