{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:16:18Z","timestamp":1785428178100,"version":"3.56.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100018542","name":"Natural Science Foundation of Sichuan Province","doi-asserted-by":"publisher","award":["2025ZNSFSC1561"],"award-info":[{"award-number":["2025ZNSFSC1561"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Research and Developmet Guidance Projects of Deyang City","award":["2024SZY001"],"award-info":[{"award-number":["2024SZY001"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Group B\n                      <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                      (GBS) is a significant pathogen that causes perinatal infections, seriously threatening the health of pregnant women and newborns. Prophylactic antibiotic treatment for pregnant women who screen positive for GBS can notably reduce the incidence and fatality of neonatal infections. Herein, we developed a\n                      <jats:underline>v<\/jats:underline>\n                      isual nucleic acid method for\n                      <jats:underline>G<\/jats:underline>\n                      BS that integrates\n                      <jats:underline>R<\/jats:underline>\n                      PA and\n                      <jats:underline>C<\/jats:underline>\n                      RISPR\/Cas12a in a\n                      <jats:underline>o<\/jats:underline>\n                      ne-\n                      <jats:underline>t<\/jats:underline>\n                      ube setup, termed VGRCOT. The VGRCOT method achieved one-tube detection by adding the appropriate reagents to the bottom and lid of the EP tube, respectively. By rigorous optimization of ssDNA-FQ reporter concentration, crRNA concentration, RPA reaction time, and CRISPR\/Cas12a cleavage time, VGRCOT can exhibit fluorescence under ultraviolet light, enabling visual detection. Under optimal conditions, VGRCOT has a satisfactory selectivity, and the detection limit was determined as 10\n                      <jats:sup>1<\/jats:sup>\n                      copies\/reaction. Finally, VGRCOT also showed good performance comparable to qPCR in the actual detection of clinical specimens. Due to its ease of operation and convenient signal acquisition, VGRCOT shows promise for point-of-care testing in reproductive health.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study presents a convenient, sensitive, and accurate visual detection method (VGRCOT) for GBS, combining RPA and CRISPR\/Cas12a in a single reaction vessel. Through optimization of experimental conditions, VGRCOT enables detection within 60 min, with a minimum detection limit of 10\n                        <jats:sup>1<\/jats:sup>\n                        copies per reaction. VGRCOT offers several advantages by adding the appropriate reagents to the bottom and lid of the EP tube. The one-tube visualization method effectively prevents aerosol contamination, simplifies procedures, and enables visual detection without complex instruments, making it ideal for resource-limited environments. Additionally, its editable crRNA and the use of commonly available laboratory reagents allow for easy reprogramming to detect various pathogens, supporting scalable and low-cost batch production.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01395-25","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T13:18:48Z","timestamp":1755177528000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["VGRCOT: a one-tube visual detection method for group B\n                    <i>Streptococcus<\/i>\n                    combining RPA and CRISPR\/Cas12a for point-of-care testing in reproductive health"],"prefix":"10.1128","volume":"13","author":[{"given":"Caixia","family":"Ji","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00pcrz470","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, People\u2019s Hospital of Deyang City, Chengdu University of Traditional Chinese Medicine","place":["Deyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqiang","family":"Ru","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Jincheng People's Hospital","place":["Jincheng, China"]},{"name":"Department of Clinical Laboratory, Jincheng Hospital Affiliated to Changzhi Medical College","place":["Jincheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiao","family":"Han","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00rd5t069","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Laboratory Medicine, School of 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