{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:24:14Z","timestamp":1788269054044,"version":"build-2803163510"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Xinjiang Uyghur Autonomous Region \"Tianchi Talent\" Youth Doctoral Talent Project","award":["BT-2025-TCYC-0066"],"award-info":[{"award-number":["BT-2025-TCYC-0066"]}]},{"name":"Project for Research Start-up for High-level Talents of Shihezi University","award":["RCZK202578"],"award-info":[{"award-number":["RCZK202578"]}]},{"name":"Central Government Guided Local Science and Technology Development Project","award":["2025YD013"],"award-info":[{"award-number":["2025YD013"]}]},{"name":"the Key Fields Science and Technology Tackling Program","award":["2025AB083, 2024AB034, 2024AB035"],"award-info":[{"award-number":["2025AB083, 2024AB034, 2024AB035"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      is an important pathogen associated with bovine mastitis and dairy product contamination, posing economic and public health risks through the food chain. In this study, a temperate phage, vB_SauL_202595, was isolated from a dairy farm environmental sample using\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      SHZ-0127 as the host, and its biological characteristics, genomic features, and antibacterial activity in dairy matrices were evaluated. vB_SauL_202595 lysed 18 of 66 tested\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      strains, with a lysis susceptibility rate of 27.3%, including 5 highly susceptible strains, indicating a relatively limited host range. The optimal multiplicity of infection was 0.01, the latent period was approximately 30 min, and the burst size was approximately 316 PFU\/cell. The phage remained stable at 4\u00b0C\u201337\u00b0C and pH 6\u201310. Genome analysis showed that vB_SauL_202595 belongs to the class\n                      <jats:italic toggle=\"yes\">Caudoviricetes<\/jats:italic>\n                      , has a genome of 44,503 bp with 33.59% GC content, and encodes 63 predicted proteins. No typical antibiotic resistance genes or major virulence factors were detected; however, integrase and repressor genes were identified, supporting its temperate nature. vB_SauL_202595 inhibited\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      SHZ-0127 growth, reduced mature biofilm biomass, and decreased viable bacterial counts in milk and yogurt, with reductions of 1.23 and 1.42 log\n                      <jats:sub>10<\/jats:sub>\n                      CFU\/mL under representative conditions, respectively. From a One Health perspective, these findings provide foundational evidence for reducing\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      contamination and related antimicrobial resistance risks along the dairy chain. Overall, vB_SauL_202595 represents a candidate phage resource for dairy-associated\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      biocontrol research, but its limited host range and lysogeny-related genes require further safety assessment before food-related applications.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>\n                        <jats:bold>IMPORTANCE<\/jats:bold>\n                      <\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                        is a major pathogen associated with bovine mastitis and a common contaminant in dairy products, causing economic losses and public health risks through the food chain. Although phage-based biocontrol has emerged as a promising strategy for controlling\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        contamination in dairy products, systematic evidence regarding phage activity in actual dairy matrices remains limited. In this study, we isolated and characterized a dairy farm environment-derived temperate phage, vB_SauL_202595, and evaluated its biological characteristics, genomic features, host range, stability, biofilm removal ability, and antibacterial performance in milk and yogurt. These findings provide foundational experimental evidence for phage-based dairy biocontrol against\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        . However, due to its limited host range and lysogeny-related genomic features, vB_SauL_202595 should be considered a candidate phage resource for further study. Broader validation, including phage-cocktail testing, long-term storage assays, product quality assessment, and regulatory safety evaluation, is needed before practical application.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01413-26","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:03:17Z","timestamp":1785330197000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, identification, and genomic characterization of\n                    <i>Staphylococcus aureus<\/i>\n                    phage vB_SauL_202595 and its bacteriostatic application in dairy products"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3464-9718","authenticated-orcid":false,"given":"Meihui","family":"Tian","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6203-1864","authenticated-orcid":false,"given":"Yaqian","family":"Liang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yize","family":"Guo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengbai","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4961-0771","authenticated-orcid":false,"given":"Kexun","family":"Lian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7725-3853","authenticated-orcid":true,"given":"Tianyi","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7146-4018","authenticated-orcid":false,"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0kvm78","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Animal Science and Technology, Shihezi University","place":["Shihezi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03197-5"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.16259"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127345"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2023.05.016"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-25238"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2022.111969"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/med4.6"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13070588"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2024.01.017"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18147602"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000530385"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12863-025-01402-6"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2024.142685"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2023.110339"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-022-02908-3"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods12213989"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2012.2615"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00037-22"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-022-02640-9"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2024.2313024"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.017"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2022.109872"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2023.04.008"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2023.0170"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10123-022-00292-3"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1516223"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13070638"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01901-24"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxad250"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1868950"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-26089"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.32607\/actanaturae.27468"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2021.198635"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-025-03895-8"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxaf008"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-25895"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2023.110525"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2023.113214"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-022-03296-9"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01413-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01413-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:11:46Z","timestamp":1788268306000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01413-26"}},"subtitle":[],"editor":[{"given":"Jeffrey A.","family":"Gralnick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,1]]}},"alternative-id":["10.1128\/spectrum.01413-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01413-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"2026-04-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01413-26"}}