{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:51:42Z","timestamp":1789584702774,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,10,31]]},"abstract":"<jats:p>With novel mutant SARS-CoV-2 variants of concern on the rise, knowledge of immune specificities against SARS-CoV-2 proteins is increasingly important for understanding the impact of structural changes in antibody-reactive protein epitopes on naturally acquired and vaccine-induced immunity, as well as broader topics of cross-reactivity and viral evolution. A multi-coronavirus protein microarray used to map the binding of COVID-19 patient antibodies to SARS-CoV-2 proteins and protein fragments as well as to the proteins of four other coronaviruses that infect humans has shown specific regions of SARS-CoV-2 proteins that are highly reactive with patient antibodies and revealed cross-reactivity of these antibodies with other human coronaviruses.<\/jats:p>","DOI":"10.1128\/spectrum.01416-21","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T10:30:59Z","timestamp":1635330659000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray"],"prefix":"10.1128","volume":"9","author":[{"given":"David","family":"Camerini","sequence":"first","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"},{"name":"University of California, Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arlo Z.","family":"Randall","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krista","family":"Trappl-Kimmons","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Oberai","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Hung","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Edgar","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Shandling","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vu","family":"Huynh","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy A.","family":"Teng","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Hermanson","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozelyn V.","family":"Pablo","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan M.","family":"Stumpf","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra N.","family":"Lester","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Harcourt","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azaibi","family":"Tamin","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Rasheed","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie J.","family":"Thornburg","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panayampalli S.","family":"Satheshkumar","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowu","family":"Liang","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard B.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Yee","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Townsend","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph J.","family":"Campo","sequence":"additional","affiliation":[{"name":"Antigen Discovery Incorporated (ADI), Irvine, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"WHO Director-General's opening remarks at the media briefing on COVID-19 - 11 March 2020. https:\/\/www.who.int\/director-general\/speeches\/detail\/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb5793"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30243-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0897-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa344"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01526-2020"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa310"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc4776"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc5343"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.07.020"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.142386"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409065102"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00671-11"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-11-82"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.6b00006"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1088-9"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjspcare-2020-002554"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.9535"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.08.005"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc5902"},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","unstructured":"de Assis RR Jain A Nakajima R Jasinskas A Felgner J Obiero JM Adenaiye O Tai S Hong F Norris PJ Stone M Simmons G Bagri A Schreiber M Buser A Holbro A Battegay M Hosimer P Noesen C Milton DK Davies DH Contestable P Corash LM Busch MP Felgner PL Khan S Prometheus Study Group. 2020. Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray. bioRxiv. 2020.04.15.043364.","DOI":"10.1101\/2020.04.15.043364"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70864-8"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.03.002"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","unstructured":"Feng Y Qiu M Liu L Zou S Li Y Luo K Guo Q Han N Sun Y Wang K Zhuang X Zhang S Chen S Mo F. 2020. Multi-epitope vaccine design using an immunoinformatics approach for 2019 novel coronavirus (SARS-CoV-2). Biorxiv. doi:10.1101\/2020.03.03.962332.","DOI":"10.1101\/2020.03.03.962332"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","unstructured":"Fast E Altman R Chen B. 2020. Potential T-cell and B-cell epitopes of 2019-nCoV. Biorxiv. doi:10.1101\/2020.02.19.955484.","DOI":"10.1101\/2020.02.19.955484"},{"key":"e_1_3_3_27_2","doi-asserted-by":"crossref","unstructured":"Khan S Nakajima R Jain A de Assis RR Jasinskas A Obiero JM Adenaiye O Tai S Hong F Milton DK Davies H Felgner PL Prometheus Study Group. 2020. Analysis of serologic cross-reactivity between common human coronaviruses and SARS-CoV-2 using coronavirus antigen microarray. Biorxiv. doi:10.1101\/2020.03.24.006544.","DOI":"10.1101\/2020.03.24.006544"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00523-5"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16638-2"},{"key":"e_1_3_3_30_2","doi-asserted-by":"crossref","unstructured":"Zhang X Wu X Wang D Lu M Hou X Wang H Liang T Dai J Duan H Xu Y Li Y Yu X. 2020. Proteome-wide analysis of differentially-expressed SARS-CoV-2 antibodies in early COVID-19 infection. Biorxiv. doi:10.1101\/2020.04.14.20064535.","DOI":"10.1101\/2020.04.14.20064535"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd4250"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2020.100123"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23977-1"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf6648"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0252628"},{"key":"e_1_3_3_36_2","unstructured":"R Core Team. 2017. R: a language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. https:\/\/www.R-project.org\/."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_3_3_38_2","doi-asserted-by":"crossref","unstructured":"Freeman B Lester S Mills L Rasheed M Moye S Abiona O Hutchinson GB Morales-Betoulle M Krapinunaya I Gibbons A Chiang C-F Cannon D Klena J Johnson JA Owen SM Graham BS Corbett KS Thornburg NJ. 2020. Validation of a SARS-CoV-2 spike protein ELISA for use in contact investigations and serosurveillance. bioRxiv. doi:10.1101\/2020.04.24.057323.","DOI":"10.1101\/2020.04.24.057323"},{"key":"e_1_3_3_39_2","unstructured":"FDA. 2021. EUA authorized serology test performance. https:\/\/www.fda.gov\/medical-devices\/coronavirus-disease-2019-covid-19-emergency-use-authorizations-medical-devices\/eua-authorized-serology-test-performance."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu393"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1198\/000313002533"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.01416-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.01416-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:55:36Z","timestamp":1646711736000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/Spectrum.01416-21"}},"subtitle":[],"editor":[{"given":"Alison","family":"Sinclair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,10,31]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,10,31]]}},"alternative-id":["10.1128\/Spectrum.01416-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01416-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.01.14.21249690","asserted-by":"object"}]},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,31]]},"assertion":[{"value":"2021-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01416-21"}}