{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:34:53Z","timestamp":1788384893950,"version":"build-2803163510"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WI1436\/13-1"],"award-info":[{"award-number":["WI1436\/13-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,6,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      High genomic plasticity within\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      enables it to acquire and accumulate genetic material through horizontal gene transfer. In this study, we sought to investigate the virulence genes, phylogroups, antibiotic resistance genes, plasmid replicons, multilocus sequence types (MLST), and core genome MLST of multidrug-resistant\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      recovered from diarrheagenic children under 5 years from Mukuru Informal Settlement in Nairobi, Kenya. A total of 39 multidrug-resistant (MDR) strains had their DNA extracted, and whole-genome sequencing was done using the Illumina HiSeq 2000 platform. Twenty-six\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      assemblies were analyzed using web-based bioinformatics tools available at the Centre for Genomic Epidemiology and EnteroBase. The isolates were categorized into four main phylogroups, where 10\/26 (38.5%) belonged to the B2 phylogroup, 4\/26 (15.4%) belonged to D, 3\/26 (11.5%) belonged to A, 1\/26 (3.8%) belonged to B1, while 8\/26 (30.8%) were not determined. FimH30 was predominantly found in the most frequent phylogroup B2 and sequence type (ST) 131. The most common beta-lactam resistance genes were\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>TEM-1B<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTXM 15<\/jats:sub>\n                      , followed by three fluoroquinolone resistance genes [\n                      <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                      6\/26 (23.1%),\n                      <jats:italic toggle=\"yes\">qnrB4<\/jats:italic>\n                      2\/26 (7.7%), and\n                      <jats:italic toggle=\"yes\">aac(6\u2032)-Ib-cr<\/jats:italic>\n                      , 8\/26 (30.8%)]. Of 26 isolates, 15 had at least one amino acid substitution in the housekeeping genes\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.S83L<\/jats:italic>\n                      ),\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.D87N<\/jats:italic>\n                      ),\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.S80I<\/jats:italic>\n                      ),\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.E84V<\/jats:italic>\n                      ),\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.S57T<\/jats:italic>\n                      ), and\n                      <jats:italic toggle=\"yes\">parE<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">p.I529L<\/jats:italic>\n                      ), associated with resistance to fluoroquinolones. A total of 40 diverse virulence genes were detected among the isolates. Thirteen different STs were isolated from the\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      genomes, which included ST 131, ST 3036, ST 38, ST 10, ST 12569, ST 15271, ST 2076, ST 311, ST 3572, ST 394, ST 453, ST 46, and ST 1722. Only two isolates (2\/26, 7.7%) from the Municipal City Council clinic were genetically related. Additionally, the most abundant plasmid replicon identified belonged to the IncF family, IncFII(pRSB107), in particular, followed by the Col family. The study highlighted the first\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      ST46 to harbor the\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM5<\/jats:sub>\n                      gene encoded in Col(BS512), IncFII(pRSB107), and IncFIB(AP001918) plasmid replicons in Kenya. We further demonstrated the diversity of MDR\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      associated with diarrhea in an endemic setting in Kenya.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This study investigated the molecular characterization of multidrug-resistant\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      isolated from diarrheagenic children under 5 years of age in Mukuru Informal Settlement in Nairobi, Kenya. This is an important addition to the genomic analysis data of multi-drug resistant diarrheal\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      in Kenya. The use of whole-genome sequencing to identify and characterize these isolates is valuable and provides valuable insights into the molecular epidemiology of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      in the region.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01420-24","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T09:01:41Z","timestamp":1747299701000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Molecular characterization of multidrug-resistant\n                    <i>E. coli<\/i>\n                    recovered from diarrheagenic children under 5 years from Mukuru Informal Settlement, Nairobi, Kenya, based on whole-genome sequencing analysis"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9369-1300","authenticated-orcid":true,"given":"Susan","family":"Kiiru","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04r1cxt79","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbiology Research, Kenya Medical Research Institute","place":["Nairobi, Kenya"]},{"id":[{"id":"https:\/\/ror.org\/015h5sy57","id-type":"ROR","asserted-by":"publisher"}],"name":"Jomo Kenyatta University of Agriculture and Technology, JKUAT","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purity","family":"Kasiano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04r1cxt79","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbiology Research, Kenya Medical Research Institute","place":["Nairobi, Kenya"]},{"id":[{"id":"https:\/\/ror.org\/015h5sy57","id-type":"ROR","asserted-by":"publisher"}],"name":"Jomo Kenyatta University of Agriculture and Technology, JKUAT","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Maina","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04r1cxt79","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbiology Research, Kenya Medical Research Institute","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Njeru","family":"Mwaniki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04r1cxt79","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbiology Research, Kenya Medical Research Institute","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edinah","family":"Songoro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/015h5sy57","id-type":"ROR","asserted-by":"publisher"}],"name":"Jomo Kenyatta University of Agriculture and Technology, JKUAT","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Kariuki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04r1cxt79","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Microbiology Research, Kenya Medical Research Institute","place":["Nairobi, Kenya"]},{"id":[{"id":"https:\/\/ror.org\/022mz6y25","id-type":"ROR","asserted-by":"publisher"}],"name":"Drugs for Neglected Diseases initiative, Eastern Africa","place":["Nairobi, Kenya"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"WHO. 2020. 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