{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:27:32Z","timestamp":1790972852560,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a promising technique for the rapid identification microorganisms. The aim of this study was to create a new database for the accurate identification of\n                      <jats:italic>Candidozyma auris<\/jats:italic>\n                      (formerly known as\n                      <jats:italic>Candida auris<\/jats:italic>\n                      ) and 11 species of the\n                      <jats:italic>Candidozyma haemuli<\/jats:italic>\n                      species complex, including\n                      <jats:italic>C. chanthaburiensis<\/jats:italic>\n                      ,\n                      <jats:italic>C. duobushaemuli<\/jats:italic>\n                      ,\n                      <jats:italic>C. haemuli<\/jats:italic>\n                      ,\n                      <jats:italic>C. heveicola<\/jats:italic>\n                      ,\n                      <jats:italic>C. khanbhai<\/jats:italic>\n                      ,\n                      <jats:italic>C. konsanensis<\/jats:italic>\n                      ,\n                      <jats:italic>C. metrosideri<\/jats:italic>\n                      ,\n                      <jats:italic>C. ohialehuae<\/jats:italic>\n                      ,\n                      <jats:italic>C. pseudohaemuli<\/jats:italic>\n                      ,\n                      <jats:italic>C. ruelliae<\/jats:italic>\n                      , and\n                      <jats:italic>C. vulturna<\/jats:italic>\n                      . Seventy-one\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      isolates from different national institutions were studied. Thirty-seven strains were used to create a MALDI-TOF (microTyper MS) database using the formic acid extraction method. The validation of this database was performed with 34 other strains of the genus\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      , and the result was compared with the identification results when using DBRs v1.0.0.4 (Tianrui, China). Our library allowed a 100% identification of the evaluated strains with all strains showing log scores of &gt;2.0. Repeatability and reproducibility tests result showed a coefficient of variation of the log score values of less than 5%. The MALDI-TOF MS system can identify\n                      <jats:italic>C. auris<\/jats:italic>\n                      and related species quickly and accurately. This method will play a crucial role in accurately diagnosing infectious agents of the genus\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      in clinical practice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Importance\n                      <jats:italic>Candidozyma auris<\/jats:italic>\n                      , also known as\n                      <jats:italic>Candida auris<\/jats:italic>\n                      , has quickly spread across the world, and prompt identification of\n                      <jats:italic>C. auris<\/jats:italic>\n                      from infected individuals is critical. However, a standard identification method is lacking for the identification of\n                      <jats:italic>C. auris<\/jats:italic>\n                      in clinical and public health laboratories. To make matters worse, its biochemical assimilation profile was found to be similar to that of closely related and even no-related species, leading to frequent misidentification. To improve diagnostics of this and closely related species, we created a database of reference mass spectra resulting in the efficient and correct identification of all\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      species by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Moreover, potential pathogenic species of\n                      <jats:italic>Candidozyma<\/jats:italic>\n                      can be effectively identified by MALDI-TOF MS, and differentiated from non-clinically relevant phylogenetic relatives. Thus, MALDI-TOF MS may help expedite laboratory diagnosis and treatment of\n                      <jats:italic>C. auris<\/jats:italic>\n                      and related species of clinical importance and help the clinician to decide on early treatment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01444-24","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T09:03:23Z","timestamp":1732007003000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Improvement of a MALDI-TOF database for the reliable identification of\n                    <i>Candidozyma auris<\/i>\n                    (formally\n                    <i>Candida auris<\/i>\n                    ) and related 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