{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:46:49Z","timestamp":1791888409896,"version":"4.3.4"},"reference-count":59,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009837","name":"Canadian Food Inspection Agency","doi-asserted-by":"publisher","award":["research project N-000209"],"award-info":[{"award-number":["research project N-000209"]}],"id":[{"id":"10.13039\/100009837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Human infections caused by\n                      <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n                      in Canada are not well documented. The following study analyzed\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      (excluding BCG) isolates identified in public health laboratories across Canadian provinces between 2000 and 2025. Whole-genome sequencing was used to catalog mutations linked to antimicrobial resistance and evaluate the genomic relatedness of isolates. The majority of samples in this study were non-respiratory (60%). Of 74 study isolates, 69 (93%) were phenotypically sensitive to first-line drugs isoniazid, rifampin, and ethambutol. The most frequently encountered\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      clusters belonged to\n                      <jats:italic toggle=\"yes\">M. tuberculosis<\/jats:italic>\n                      complex lineages La 1.7 and La 1.8. Although some isolates showed phylogenetic clustering, their lack of spatial and temporal relatedness suggested sporadic cases rather than local\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      transmission. Of all first-line drugs evaluated, isoniazid showed the lowest concordance (94.4%) between phenotypic and genotypic methods. The isolates in this study harbor some mutations that are not currently documented in the WHO mutation catalog, indicating that these variants may be rare. The findings suggest that the prevalence of\n                      <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                      infection in Canada is low with cases unlikely to be acquired through human-to-human transmission.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Incidence of human infections due to\n                        <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n                        in Canada is largely unknown as these data are not collected or annually published. This research is a first effort to identify historical\n                        <jats:italic toggle=\"yes\">M. bovis<\/jats:italic>\n                        isolates that were available for culture, had drug susceptibility testing data, and were analyzed through whole-genome sequencing. The annual reporting of these data in Canada holds value.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01485-26","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:00:14Z","timestamp":1791885614000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Genomic diversity of human-associated\n                    <i>Mycobacterium bovis<\/i>\n                    in Canada"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6020-9995","authenticated-orcid":true,"given":"Meenu K.","family":"Sharma","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023xf2a37","id-type":"ROR","asserted-by":"publisher"}],"name":"National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Public Health Agency of Canada","place":["Winnipeg, 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