{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T10:39:04Z","timestamp":1786963144124,"version":"build-2736575974"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100018916","name":"Suzhou Municipal Health Commission","doi-asserted-by":"publisher","award":["SZWJ2023a056"],"award-info":[{"award-number":["SZWJ2023a056"]}],"id":[{"id":"10.13039\/100018916","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Bacterial meningitis is a disease with high mortality and morbidity, and it primarily manifests as symptoms involving the central nervous system (CNS). Hence, it would be of great importance to make an early diagnosis and initiate empirical antimicrobial treatment in time for this disease. In this study, we investigated the feasibility of rapid\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      ) identification and drug resistance analysis through the combination of centrifugation-based enrichment of bacteria and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Specifically, the cerebrospinal samples were treated by differential centrifugation to collect precipitates after a short-term rapid culture. Then, the precipitates were identified by MALDI-TOF MS. Subsequently, the bacterial solution (10\n                      <jats:sup>6<\/jats:sup>\n                      CFU\/mL) was mixed with an equal volume of cation-adjusted Mueller-Hinton broth (CAMHB) supplemented with cefoxitin (4 \u00b5g\/mL). After the culture of the mixture, the precipitates obtained by centrifugation were analyzed by MALDI-TOF MS. The efficiency of centrifugation-based enrichment of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      was 87.9% at 10\n                      <jats:sup>3<\/jats:sup>\n                      CFU\/mL and increased to 90.4% at 10\n                      <jats:sup>2<\/jats:sup>\n                      CFU\/mL. This identification efficiency reached 100% after an 8-h culture. The optimal testing time for bacterial resistance identification was achieved by the culture within 3 h. The validity, sensitivity, and specificity were all 100% at this time point. The results of the rapid identification method were identical to those of the broth microdilution method. Through this protocol, the identification and drug resistance analysis of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      in the cerebrospinal fluid (CSF) can be completed within 11 h. These findings are expected to provide a new method for the rapid diagnosis and treatment of patients with bacterial meningitis.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study introduces a novel method combining centrifugation-based bacterial enrichment with MALDI-TOF MS, enabling an 11-h identification of\n                        <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                        and direct detection of methicillin resistance directly from the CSF. Validated with 100% sensitivity and specificity, this approach accelerates targeted antibiotic therapy and establishes a streamlined workflow for rapid diagnosis and treatment of bacterial meningitis.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01508-25","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T13:00:36Z","timestamp":1775221236000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Rapid identification and methicillin resistance test to\n                    <i>Staphylococcus aureus<\/i>\n                    in cerebrospinal fluid by MALDI-TOF MS"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4853-4532","authenticated-orcid":true,"given":"Mengyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanxuan","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03t1yn780","id-type":"ROR","asserted-by":"publisher"}],"name":"The First Affiliated Hospital of Anhui Medical University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pbexw16","id-type":"ROR","asserted-by":"publisher"}],"name":"TongLing People's Hospital","place":["Tongling, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Ma","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuguo","family":"Qin","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Hu","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henggui","family":"Hu","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolei","family":"Du","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7521-5062","authenticated-orcid":false,"given":"Kaixuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5522-7382","authenticated-orcid":false,"given":"Sudi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6340-2632","authenticated-orcid":false,"given":"Yuanyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Wanbei Coal Electricity Group General Hospital","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.74"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2022.20521"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2024.24802"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2018.11.008"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01949-18"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2020.08.002"},{"key":"e_1_3_5_8_2","first-page":"3","volume-title":"Tackling threats and future problems of multidrug-resistant bacteria","author":"Stadler M","year":"2016","unstructured":"Stadler M, Dersch P. 2016. 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