{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:03:43Z","timestamp":1791396223569,"version":"4.3.3"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100013093","name":"Science and Technology Planning Project of Shenzen Municipality","doi-asserted-by":"crossref","award":["JCYJ20190809101409603"],"award-info":[{"award-number":["JCYJ20190809101409603"]}],"id":[{"id":"10.13039\/501100013093","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013093","name":"Science and Technology Planning Project of Shenzen Municipality","doi-asserted-by":"crossref","award":["JCYJ20220530160206014"],"award-info":[{"award-number":["JCYJ20220530160206014"]}],"id":[{"id":"10.13039\/501100013093","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82201793"],"award-info":[{"award-number":["82201793"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research Foundation of PEKING UNIVERSITY SHENZHEN HOSPITAL","award":["KYQD2022145, KYQD202211"],"award-info":[{"award-number":["KYQD2022145, KYQD202211"]}]},{"name":"Shenzhen High-level Hospital Construction","award":["YBH2019-260"],"award-info":[{"award-number":["YBH2019-260"]}]},{"name":"Shenzhen Key Medical Discipline Construction","award":["no. 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In this study, we recruited 31 RVVC patients, 28 VVC patients, and 29 healthy women. Vaginal samples were collected for metagenomic and metabolic analysis. RVVC and VVC groups presented similar clinical symptoms, with only a significantly increased incidence of swelling in the VVC group. Vaginal microbiota in VVC\/RVVC exhibited a decreased abundance of\n              <jats:italic>Lactobacillus<\/jats:italic>\n              and increased bacterial vaginosis-associated bacteria, such as\n              <jats:italic>Gardnerella<\/jats:italic>\n              ,\n              <jats:italic>Prevotella<\/jats:italic>\n              , and\n              <jats:italic>Atopobium<\/jats:italic>\n              . Notably,\n              <jats:italic>Lactobacillus iners<\/jats:italic>\n              was higher in RVVC, suggesting not all\n              <jats:italic>Lactobacillus<\/jats:italic>\n              species are protective. Healthy women showed lower overall microbiota diversity, emphasizing single-species dominance for stability. Glycogen metabolism pathways were enriched in RVVC\/VVC, and were correlated with\n              <jats:italic>Atopobium vaginae<\/jats:italic>\n              ,\n              <jats:italic>Prevotella bivia<\/jats:italic>\n              , and\n              <jats:italic>Lactobacillus jensenii<\/jats:italic>\n              . Peptidoglycan synthesis pathways, associated with\n              <jats:italic>P. bivia<\/jats:italic>\n              , were enriched, with the substrate L-glutamate elevated in RVVC, possibly promoted by\n              <jats:italic>L. iners<\/jats:italic>\n              . These findings shed light on potential therapeutic targets for recurrent VVC, contributing to the understanding of the intricate interplay between the metabolism of vaginal microbiome and disease.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study enhances our knowledge of the vaginal microbiota dynamics and the role of associated metabolites in individuals with vulvovaginal candidiasis (VVC) and recurrent vulvovaginal candidiasis through shotgun sequencing and multi-omics analysis. The relationship between metabolites and vaginal microbiota and disease state was revealed. The accumulation of L-glutamate generated in glycogen metabolism, which is governed by\n              <jats:italic>Lactobacillus iners<\/jats:italic>\n              or bacterial vaginosis-associated bacteria, may contribute to the incidence and recurrence of VVC. Such insights have the potential to impact the treatment and prevention strategies for these common yet distressing conditions, potentially leading to targeted therapies and improved patient outcomes.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01521-24","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T15:03:41Z","timestamp":1730300621000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Highlight signatures of vaginal microbiota and metabolome contributed to the occurrence and recurrence of vulvovaginal candidiasis"],"prefix":"10.1128","volume":"12","author":[{"given":"Yiheng","family":"Liang","sequence":"first","affiliation":[{"name":"Center of Obstetrics and Gynecology, Peking University Shenzhen Hospital","place":["Shenzhen, China"]},{"name":"Shenzhen Key Laboratory on Technology for Early Diagnosis of Major, Gynaecological Disease","place":["Shenzhen, China"]},{"name":"Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoqi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, School of Life Sciences, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangrong","family":"Fan","sequence":"additional","affiliation":[{"name":"Center of Obstetrics and Gynecology, Peking University Shenzhen Hospital","place":["Shenzhen, China"]},{"name":"Shenzhen Key Laboratory on Technology for Early Diagnosis of Major, Gynaecological Disease","place":["Shenzhen, China"]},{"name":"Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changzhong","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Obstetrics and Gynecology, Peking University Shenzhen Hospital","place":["Shenzhen, China"]},{"name":"Shenzhen Key Laboratory on Technology for Early Diagnosis of Major, Gynaecological Disease","place":["Shenzhen, China"]},{"name":"Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Huang","sequence":"additional","affiliation":[{"name":"Center of Obstetrics and Gynecology, Peking University Shenzhen Hospital","place":["Shenzhen, China"]},{"name":"Shenzhen Key Laboratory on Technology for Early Diagnosis of Major, Gynaecological Disease","place":["Shenzhen, China"]},{"name":"Institute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center","place":["Shenzhen, 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