{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:49:39Z","timestamp":1791924579687,"version":"4.3.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["51AU40_180541"],"award-info":[{"award-number":["51AU40_180541"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI158388"],"award-info":[{"award-number":["R01AI158388"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"German Federal Ministry of Education and Research (BMBF) via a project of the Deutsche Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TTU09.818"],"award-info":[{"award-number":["TTU09.818"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AntiResist: New approaches to combat antibiotic-resistant bacteria","award":["51AU40_180541"],"award-info":[{"award-number":["51AU40_180541"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,12,22]]},"abstract":"<jats:p>Therapeutic options to combat Gram-negative bacterial pathogens are dwindling with increasing antibiotic resistance. This study presents a proof of concept for the heterologous-expression approach to expand on the novel antibiotic class of darobactins and to generate analogs with different activities and pharmacokinetic properties.<\/jats:p>","DOI":"10.1128\/spectrum.01535-21","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T11:25:32Z","timestamp":1640172332000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Mutasynthetic Production and Antimicrobial Characterization of Darobactin Analogs"],"prefix":"10.1128","volume":"9","author":[{"given":"Nils","family":"B\u00f6hringer","sequence":"first","affiliation":[{"name":"Justus-Liebig-University Gie\u00dfen, Gie\u00dfen, Germany"},{"name":"German Center of Infection Research (DZIF), Partner Site Gie\u00dfen-Marburg-Langen, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Green","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Justus-Liebig-University Gie\u00dfen, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Mettal","sequence":"additional","affiliation":[{"name":"Justus-Liebig-University Gie\u00dfen, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Marner","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch for Bioresources, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Majed","family":"Modaresi","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman P.","family":"Jakob","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zerlina G.","family":"Wuisan","sequence":"additional","affiliation":[{"name":"Justus-Liebig-University Gie\u00dfen, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timm","family":"Maier","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Iinishi","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Hiller","sequence":"additional","affiliation":[{"name":"Biozentrum, University of Basel, Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Lewis","sequence":"additional","affiliation":[{"name":"Antimicrobial Discovery Center, Department of Biology, Northeastern University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9947-8079","authenticated-orcid":true,"given":"Till F.","family":"Sch\u00e4berle","sequence":"additional","affiliation":[{"name":"Justus-Liebig-University Gie\u00dfen, Gie\u00dfen, Germany"},{"name":"German Center of Infection Research (DZIF), Partner Site Gie\u00dfen-Marburg-Langen, Gie\u00dfen, Germany"},{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch for Bioresources, Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0125-x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.056"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1791-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2021.04.007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/sb500351f"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03455-w"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg301"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1cc11574j"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.04.007"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV119.006545"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/d0ob01677b"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.858"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.34"},{"key":"e_1_3_3_16_2","unstructured":"Anonymous. 2019. Antibiotic resistance threats in the United States 2019. Centers for Disease Control and Prevention Atlanta GA."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp405418y"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1449"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12125"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-07"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep08643"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020007"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1611594113"},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","unstructured":"Gibson DG Young L Chuang RY Venter JC Hutchison CA Smith HO. 2009. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343\u2013345. doi:10.1038\/nmeth.1318.","DOI":"10.1038\/nmeth.1318"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909029436"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1107\/s0907444902016657"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909042073"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3235"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b03220"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c00917"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu830"},{"key":"e_1_3_3_36_2","unstructured":"Smith L. 2021. Protocol for protein binding rapid equilibrium dialysis (RED) assay in mouse plasma. Bioassay Record. PubChem AID 1937. Burnham Center for Chemical Genomics. https:\/\/pubchem.ncbi.nlm.nih.gov\/bioassay\/1937."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00640"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01535-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01535-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:57:03Z","timestamp":1646711823000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01535-21"}},"subtitle":[],"editor":[{"given":"Neha","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,22]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,12,22]]}},"alternative-id":["10.1128\/spectrum.01535-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01535-21","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,22]]},"assertion":[{"value":"2021-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01535-21"}}