{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:12:53Z","timestamp":1785409973964,"version":"3.56.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study analyzed\n                      <jats:italic toggle=\"yes\">Neisseria gonorrhoeae<\/jats:italic>\n                      (GC) and\n                      <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n                      (Mgen) from remnant nucleic acid amplification test (NAAT) specimens in Nunavut, Canada (2020\u20132023) to assess strain distribution, antimicrobial resistance (AMR), and co-infection rates. Among 2,221 GC-positive specimens, no cephalosporin resistance was detected, but intermediate-to-decreased susceptibility increased in 2023. Predicted ciprofloxacin resistance was 45%, and azithromycin resistance remained stable at 9.6%. Of 778 tested specimens, 31.4% were co-infected with Mgen, with 49.2% associated with azithromycin resistance, but none with moxifloxacin resistance. These findings highlight emerging AMR trends and support reassessment of current treatment guidelines. Remnant urine specimens submitted for nucleic acid amplification testing identified differences in the prevalence and AMR patterns in GC and Mgen in a large Canadian Arctic territory.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Nunavut has the highest gonorrhea rates in Canada, yet antimicrobial resistance (AMR) surveillance is limited to culture-based testing. This study bridges that gap by using molecular assays to predict AMR directly from remnant nucleic acid amplification test specimens, providing data for remote regions that lack the resources to culture and transport\n                        <jats:italic toggle=\"yes\">Neisseria gonorrhoeae<\/jats:italic>\n                        cultures. Findings highlight relevant AMR trends for gonorrhea treatment. Mgen, despite increasing clinical prevalence and high co-infection rates with other sexually transmitted infections, is not included in national surveillance programs, furthering the need for extended monitoring.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01553-25","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T13:01:08Z","timestamp":1755090068000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of\n                    <i>Neisseria gonorrhoeae<\/i>\n                    and\n                    <i>Mycoplasma genitalium<\/i>\n                    from nucleic acid amplification test specimens, Nunavut region of Inuit Nunangat, Canada, 2020\u20132023"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4260-5225","authenticated-orcid":false,"given":"Norman","family":"Barairo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023xf2a37","id-type":"ROR","asserted-by":"publisher"}],"name":"National Microbiology Laboratory Branch, Public Health Agency of Canada","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2627-3704","authenticated-orcid":true,"given":"Ameeta E.","family":"Singh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0160cpw27","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, University of Alberta","place":["Edmonton, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6432-9257","authenticated-orcid":false,"given":"Shelley","family":"Peterson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023xf2a37","id-type":"ROR","asserted-by":"publisher"}],"name":"National Microbiology Laboratory Branch, Public Health Agency of Canada","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekua","family":"Agyemang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03wf6h922","id-type":"ROR","asserted-by":"publisher"}],"name":"Government of Nunavut","place":["Iqaluit, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3941-5583","authenticated-orcid":true,"given":"Irene","family":"Martin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023xf2a37","id-type":"ROR","asserted-by":"publisher"}],"name":"National Microbiology Laboratory Branch, Public Health Agency of Canada","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"Public Health Agency of Canada. 2023. Chlamydia gonorrhea and infectious syphilis in Canada 2021: Surveillance report. Government of Canada. Available from: https:\/\/www.canada.ca\/en\/public-health\/services\/publications\/diseases-conditions\/chlamydia-gonorrhea-infectious-syphilis-2021-surveillance-data.html"},{"key":"e_1_3_4_3_2","unstructured":"Nunavut Tunngavik Incorporated. 2024. Annual report on the state of inuit culture and society 2007\u20138. Available from: https:\/\/www.tunngavik.com\/publications\/annual-report-on-the-state-of-inuit-culture-and-society-2007-2008"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2706.204407"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.14745\/ccdr.v51i04a03"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035593"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa360"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/383047"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv274"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.029694"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040023"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30154-7"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001402"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2017-016300"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz297"},{"key":"e_1_3_4_19_2","unstructured":"World Health Organization Department of Reproductive Health and Research. 2012. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. World Health Organization. https:\/\/www.who.int\/publications\/i\/item\/9789241503501."}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01553-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01553-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:04:51Z","timestamp":1759842291000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01553-25"}},"subtitle":[],"editor":[{"given":"Ayush","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,7]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10,7]]}},"alternative-id":["10.1128\/spectrum.01553-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01553-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,7]]},"assertion":[{"value":"2025-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01553-25"}}