{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:31:12Z","timestamp":1788449472086,"version":"build-2803163510"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,6,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              There is a positive causality between coffee consumption and osteoarthritis (OA); however, whether gut microbiota is involved needs to be discussed. Here, we observed that in caffeine consumers, fecal\n              <jats:italic toggle=\"yes\">Prevotella copri<\/jats:italic>\n              abundance was positively correlated with subchondral bone mass, serum caffeine concentration was negatively correlated with bone mass, and fecal\n              <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n              was negatively correlated with serum caffeine. In the OA model, caffeine intake aggravated articular cartilage destruction, bone mass loss, and intestinal barrier damage; on the contrary, paraxanthine intake reversed the above lesions. Importantly, after the intestinal\n              <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n              supplement, caffeine-induced lesions in OA mice were effectively alleviated. Mechanically,\n              <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n              has the potential to metabolize caffeine into paraxanthine, and this effect could alleviate the ferroptosis of osteoblast in the OA model. This study screened out that\n              <jats:italic toggle=\"yes\">P. copri,<\/jats:italic>\n              an endogenous bacteria, has the ability to metabolize caffeine and revealed its effects on OA progression.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                There is positive causality between coffee consumption and osteoarthritis (OA). Caffeine exposure is responsible for the reduction of bone mass and restrained osteoblast function.\n                <jats:italic toggle=\"yes\">Prevotella copri<\/jats:italic>\n                abundance is exhausted in gut and positively correlated with subchondral bone mass in coffee consumption patients with OA. Supplement of intestinal\n                <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                alleviates caffeine-induced subchondral bone loss.\n                <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                has the potential to metabolize caffeine into paraxanthine, and this effect alleviates ferroptosis of osteoblast. Our study illustrated that intestinal\n                <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                possibly serves as a novel promising treatment for coffee consumers with OA.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01575-24","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T13:00:29Z","timestamp":1745499629000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["<i>Prevotella copri<\/i>\n            -mediated caffeine metabolism involves ferroptosis of osteoblasts in osteoarthritis"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-5359","authenticated-orcid":false,"given":"Feng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, 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