{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:14:47Z","timestamp":1753881287584,"version":"3.41.2"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32373040"],"award-info":[{"award-number":["32373040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Neospora caninum<\/jats:italic>\n              is one of the most common pathogens causing reproductive failure in ruminants (e.g., cattle and goats) worldwide. However, due to a poor understanding of the pathogenic mechanisms of\n              <jats:italic>N. caninum<\/jats:italic>\n              infection, no effective drugs and vaccines are currently available. Long non-coding RNAs (lncRNAs) have been reported to be important regulators involved in a great number of physiological and pathological processes. Our previous study found that\n              <jats:italic>N. caninum<\/jats:italic>\n              infection induced significantly aberrant expression of lncRNA profiles in caprine endometrial epithelial cells (EECs). In the present study, we found that\n              <jats:italic>N. caninum<\/jats:italic>\n              infection specifically suppressed the expression of a novel lncRNA,\n              <jats:italic>XR_001919077.1<\/jats:italic>\n              , and knockdown of\n              <jats:italic>XR_001919077.1<\/jats:italic>\n              with small interfering RNA significantly promoted the propagation of\n              <jats:italic>N. caninum<\/jats:italic>\n              in caprine EECs. Rapid amplification of cDNA ends analysis generated six splice variants of\n              <jats:italic>XR_001919077.1<\/jats:italic>\n              , with lengths ranging from 592 to 694 nt. Transfection of the full length of each variant markedly inhibited the propagation of\n              <jats:italic>N. caninum<\/jats:italic>\n              in caprine EECs. Further study suggested that\n              <jats:italic>XR_001919077.1<\/jats:italic>\n              acted as a sponge of Chi-miR-93-5p to promote the expression of\n              <jats:italic>sirt1<\/jats:italic>\n              , and the\n              <jats:italic>XR_001919077.1<\/jats:italic>\n              \/Chi-miR-93-5p\/\n              <jats:italic>sirt1<\/jats:italic>\n              axis significantly delayed the\n              <jats:italic>in vitro<\/jats:italic>\n              growth of\n              <jats:italic>N. caninum<\/jats:italic>\n              in caprine EECs by regulating host cell mitochondrial function and autophagy. Our findings provide a novel insight to understand the interactions between\n              <jats:italic>N. caninum<\/jats:italic>\n              and host cells.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                The uterus is an indispensable reproductive organ for embryo implantation and fetal growth. The endometrium is more vulnerable to infection by pathogenic microorganisms resulting in an increased risk of miscarriage.\n                <jats:italic>Neospora caninum<\/jats:italic>\n                is one of the most common pathogens causing miscarriage in ruminants and is able to naturally inhabit the uterus, with\n                <jats:italic>N. caninum<\/jats:italic>\n                tissue cysts found in the endometrium. Recent advances in\n                <jats:italic>N. caninum<\/jats:italic>\n                research have revealed aberrant expression of long non-coding RNA (lncRNA) profiles in infected caprine endometrial epithelial cells. In the present study,\n                <jats:italic>N. caninum<\/jats:italic>\n                , but not\n                <jats:italic>Toxoplasma gondii,<\/jats:italic>\n                which has similar morphological and biological features to\n                <jats:italic>N. caninum<\/jats:italic>\n                , specifically suppresses the expression of a host lncRNA,\n                <jats:italic>XR_ 001919077.1<\/jats:italic>\n                , to impair host\u2019s defense through the competitive endogenous RNA mechanism to modulate the host cell mitochondrial function and autophagy to facilitate parasite propagation. The findings suggest a novel immune evasion strategy of\n                <jats:italic>N. caninum<\/jats:italic>\n                to facilitate intracellular propagation and provide an alternative path to develop control strategies against neosporosis.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01580-24","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T14:00:16Z","timestamp":1734962416000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Neospora caninum<\/i>\n            infection specifically suppresses the expression of a host lncRNA\n            <i>XR_001919077.1<\/i>\n            to facilitate parasite propagation by modulating host cell mitochondrial function and 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