{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:36:12Z","timestamp":1791873372821,"version":"4.3.4"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271593"],"award-info":[{"award-number":["82271593"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["22120240281"],"award-info":[{"award-number":["22120240281"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Shanghai under the Shanghai Action Plan for Science, Technology and Innovation","award":["24ZR1469200"],"award-info":[{"award-number":["24ZR1469200"]}]},{"name":"The Tongji University Medicine-X Interdisciplinary Research Initiative","award":["2025-0554-YB-18"],"award-info":[{"award-number":["2025-0554-YB-18"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,1,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Skin wound repair constitutes a sophisticated biological process involving spatiotemporally coordinated molecular cascades, with emerging evidence highlighting the dynamic regulatory role of skin microbiota. Utilizing a broad-spectrum antibiotic (ABX)-treated murine model, we identified\n                      <jats:italic toggle=\"yes\">Prevotella copri<\/jats:italic>\n                      as a core functional commensal in the wound microecosystem that orchestrates tissue regeneration through metabolite-host crosstalk. ABX-induced microbial remodeling significantly enriched\n                      <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                      relative abundance, accelerated wound closure, and upregulated pro-regenerative factors vascular endothelial growth factor and epidermal growth factor. Metabolomic profiling revealed that\n                      <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                      -secreted sphingosine undergoes bioconversion to C18-ceramide via the non-canonical CerS1 pathway, driving keratinocyte hyperproliferation and neoangiogenesis. Pharmacological inhibition of CerS1 with P053 suppressed ceramide synthesis and delayed healing, mechanistically validating the sphingosine-CerS1-ceramide axis. Crucially,\n                      <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                      exhibits dual regulatory modalities: ecologically, \u03b2-lactamase-mediated antibiotic resistance establishes microbial dominance, while metabolically, sphingolipid-driven spatiotemporal signaling remodels the regenerative microenvironment. These findings align with and extend the evolving perspective of a functional wound microbiota and propose a potential synergistic strategy that combines targeted enrichment of beneficial commensals like\n                      <jats:italic toggle=\"yes\">P. copri<\/jats:italic>\n                      with metabolic axis modulation to promote healing. Our findings elucidate a microecology-metabolism circuit that transitions wound management from passive anti-infection to precision intervention, providing a molecular blueprint for developing microbiome-reprogramming therapies in regenerative medicine.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Traditional wound repair research often focuses on microbial diversity, neglecting the critical role of specific taxa in tissue regeneration. Our study challenges this by highlighting\n                        <jats:italic toggle=\"yes\">Prevotella copri<\/jats:italic>\n                        as a key species in wound healing, operating through the\n                        <jats:italic toggle=\"yes\">Prevotella copri<\/jats:italic>\n                        -sphingosine-CerS1-ceramide signaling pathway. This discovery reshapes the understanding of microbiome-host interactions and paves the way for precision microbial therapies. By showing that a single bacterium can replace complex community dynamics, we connect ecological theory with regenerative applications, offering a strategy to use microbial metabolism for precise wound healing.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01587-25","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T14:02:07Z","timestamp":1763128927000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["<i>Prevotella copri<\/i>\n                    facilitates wound healing in mice through the sphingosine-CerS1-ceramide metabolic pathway"],"prefix":"10.1128","volume":"14","author":[{"given":"Meili","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Regenerative Medicine, State Key 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