{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:59:37Z","timestamp":1789088377494,"version":"build-2803163510"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T00:00:00Z","timestamp":1640131200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,12,22]]},"abstract":"<jats:p>Despite the eradication of variola virus (VARV), the causative agent of smallpox, poxviruses still represent an important threat to human health due to their possible use as bioterrorism agents and the emergence of zoonotic poxvirus diseases. Recombinant vaccinia viruses (rVV) expressing easily traceable fluorescent or luciferase reporter genes have significantly contributed to the progress of poxvirus research.<\/jats:p>","DOI":"10.1128\/spectrum.01601-21","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T10:32:27Z","timestamp":1637749947000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Bi-Reporter Vaccinia Virus for Tracking Viral Infections\n                    <i>In Vitro<\/i>\n                    and\n                    <i>In Vivo<\/i>"],"prefix":"10.1128","volume":"9","author":[{"given":"Kevin","family":"Chiem","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA"},{"name":"Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria M.","family":"Lorenzo","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Centro Nacional Instituto de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA CSIC), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Rangel-Moreno","sequence":"additional","affiliation":[{"name":"Division of Allergy\/Immunology and Rheumatology, Department of Medicine, University of Rochester, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria De La Luz","family":"Garcia-Hernandez","sequence":"additional","affiliation":[{"name":"Division of Allergy\/Immunology and Rheumatology, Department of Medicine, University of Rochester, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Gyu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA"},{"name":"Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2424-7900","authenticated-orcid":true,"given":"Aitor","family":"Nogales","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA"},{"name":"Animal Health Research Centre (CISA), Centro Nacional Instituto de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA, CSIC), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Blasco","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Centro Nacional Instituto de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA CSIC), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7084-0804","authenticated-orcid":true,"given":"Luis","family":"Mart\u00ednez-Sobrido","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA"},{"name":"Texas Biomedical Research Institute, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010199"},{"key":"e_1_3_2_3_2","first-page":"773","article-title":"Bioterrorism: a new frontier for drug discovery and development","volume":"6","author":"Shailubhai K","year":"2003","unstructured":"Shailubhai K. 2003. Bioterrorism: a new frontier for drug discovery and development. IDrugs 6:773\u2013780.","journal-title":"IDrugs"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.06.005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01683-19"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2018.00241"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.055921-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.07.015"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4050688"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.02.009"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3791\/51522"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2011.04.014"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01376-08"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.10.4802-4813.2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147723"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8070179"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2014.12.006"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2015.11.014"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2013.00062"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7102873"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00714-17"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00969-13"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2012.07.014"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/cb3002478"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-1759(98)00156-2"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-1-10"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52053-4"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.2032388"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.5.1667"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1039\/C3MD00288H"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02381-13"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121491"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.3791\/59890"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00032-19"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00307-15"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2171"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02149-19"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4074"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-789-0:015"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.2144\/97236st07"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/9780471729259.mc14a03s39","article-title":"Preparation of cell cultures and vaccinia virus stocks","volume":"39","author":"Cotter CA","year":"2015","unstructured":"Cotter CA, Earl PL, Wyatt LS, Moss B. 2015. Preparation of cell cultures and vaccinia virus stocks. Curr Protoc Microbiol 39:14A.3:1\u201314A.3.18.","journal-title":"Curr Protoc Microbiol"},{"key":"e_1_3_2_42_2","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council (U.S.). Committee for the Update of the Guide for the Care and Use of Laboratory Animals., Institute for Laboratory Animal Research (U.S.), National Academies Press (U.S.)","year":"2011","unstructured":"National Research Council (U.S.). Committee for the Update of the Guide for the Care and Use of Laboratory Animals., Institute for Laboratory Animal Research (U.S.), National Academies Press (U.S.). 2011. Guide for the care and use of laboratory animals, 8th ed National Academies Press, Washington, DC.","edition":"8"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.3791\/55898"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01206-18"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01565-14"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188251"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-9-123"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.01601-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.01601-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:45:16Z","timestamp":1646711116000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/Spectrum.01601-21"}},"subtitle":[],"editor":[{"given":"Clinton J.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,12,22]]},"references-count":47,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,12,22]]}},"alternative-id":["10.1128\/Spectrum.01601-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01601-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.08.24.457594","asserted-by":"object"}]},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,22]]},"assertion":[{"value":"2021-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01601-21"}}