{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:47:00Z","timestamp":1753883220359,"version":"3.41.2"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Canadian Government | Canadian Institutes of Health Research","award":["FND167283"],"award-info":[{"award-number":["FND167283"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Although silver is known for its antibacterial activity, its exact mode of action remains unclear. In our previous work, we described AgNbO\n              <jats:sub>3<\/jats:sub>\n              nanoparticles (AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs) prepared using a ceramic method, followed by high-energy and low-energy ball-milling processes, which exhibited antimicrobial activity with negligible release of Ag\n              <jats:sup>+<\/jats:sup>\n              in deionized water. Here, we investigated thoroughly the mode of action of these AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs against\n              <jats:italic>Escherichia coli<\/jats:italic>\n              . Drastic morphological changes in\n              <jats:italic>E. coli<\/jats:italic>\n              were observed after their exposure to AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs. In addition to cellular damage, AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs induced the production of reactive oxygen species and lipid peroxidation, likely following the release of small amounts of Ag\n              <jats:sup>+<\/jats:sup>\n              . This was concluded from the characterization of mutants resistant to AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs that showed cross-resistance to AgNO\n              <jats:sub>3<\/jats:sub>\n              , impaired reactive oxygen species production and lipid peroxidation, and harbored a key mutation in a two-component regulatory system regulating an Ag\n              <jats:sup>+<\/jats:sup>\n              efflux pump. We calculated, however, that the quantity of Ag\n              <jats:sup>+<\/jats:sup>\n              released from AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs is not sufficient by itself to lead to bacterial death. We propose that bacterial contact with the AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs in combination with Ag\n              <jats:sup>+<\/jats:sup>\n              release is necessary for the mode of action of AgNbO\n              <jats:sub>3<\/jats:sub>\n              NPs.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Silver is known for its antibacterial activity, but its exact mode of action remains unclear. Here, we investigated thoroughly the mode of action of AgNbO\n                <jats:sub>3<\/jats:sub>\n                nanoparticles against\n                <jats:italic>Escherichia coli<\/jats:italic>\n                . Our data suggest that AgNbO\n                <jats:sub>3<\/jats:sub>\n                nanoparticles have dual effects on the cell and that both are required for its lethal action.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01648-24","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T14:05:24Z","timestamp":1733839524000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Mode of action of silver-based perovskite against Gram-negative bacteria"],"prefix":"10.1128","volume":"13","author":[{"given":"Fereshteh","family":"Fani","sequence":"first","affiliation":[{"name":"D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Centre de recherche en infectiologie du Centre de Recherche CHU de Qu\u00e9bec, Universit\u00e9 Laval","place":["Qu\u00e9bec, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cyrus","family":"Talebpour","sequence":"additional","affiliation":[{"name":"Department of Mining, Metallurgical and Materials Engineering, Universit\u00e9 Laval","place":["Qu\u00e9bec, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Leprohon","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Centre de recherche en infectiologie du Centre de Recherche CHU de Qu\u00e9bec, Universit\u00e9 Laval","place":["Qu\u00e9bec, Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Salimnia","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Children\u2019s Hospital of Michigan, Wayne State University, Detroit Medical Center","place":["Detroit, 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