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Due to its increasing carbapenem resistance, the World Health Organization has classified it as a critical priority pathogen. This study performed a comparative genomic analysis of 20 carbapenem-resistant\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      clinical strains isolated from the Hospital Infantil de M\u00e9xico Federico G\u00f3mez (CRAB-HIMFG), alongside 11 genomes from other Mexican strains. The pangenome was determined to be open, and core genome single-nucleotide polymorphism-based analysis grouped the CRAB-HIMFG strains within CC758\/IC5 and CC92\/IC2. A novel sequence type (ST) in the MLST-Pasteur scheme was identified, related to ST\n                      <jats:sup>Pas<\/jats:sup>\n                      156, and in the MLST-Oxford scheme, associated with ST\n                      <jats:sup>Oxf<\/jats:sup>\n                      758 and ST\n                      <jats:sup>Oxf<\/jats:sup>\n                      1054. Virulence and resistance genes comprised 0.61% to 2.23% of the pangenome. Oxacillinase genes and efflux pumps primarily mediated carbapenem resistance, while virulence genes included those encoding biofilm and type IV pili. Capsule typing revealed a correlation with established international clones, IC2 and IC5. Plasmids exhibited high diversity, harboring maintenance modules and toxin-antitoxin systems, with the dissemination of resistance genes linked to insertion sequences. Biofilm formation and twitching motility were not always expressed, as they depend on additional environmental factors. Our study shows that comparative genomics is an essential tool to analyze clinically and epidemiologically significant genomes, providing critical insights into gene distribution, genomic architecture, and horizontal gene transfer mechanisms in microbial populations.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        In recent years, a reported increase in the mortality rate associated with infections caused by\n                        <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                        , along with a rise in carbapenem resistance, poses a serious clinical challenge. The WHO considered this microorganism critical for research into alternative therapies and epidemiological surveillance. Despite advances in bioinformatics, genomic studies have yet to fully elucidate the structural rearrangements and secretion systems of\n                        <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                        . This knowledge gap hinders our understanding of its remarkable genomic plasticity and its ability to acquire and spread resistance and virulence genes through horizontal gene transfer.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01676-25","type":"journal-article","created":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T13:12:16Z","timestamp":1759756336000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Comparative genomics of carbapenem-resistant\n                    <i>Acinetobacter baumannii<\/i>\n                    isolated from pediatric patients in a tertiary care hospital"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8863-3456","authenticated-orcid":true,"given":"Dafne","family":"Guill\u00e9n-Navarro","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00nzavp26","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de M\u00e9xico Federico G\u00f3mez","place":["Ciudad de Mexico, Mexico"]},{"name":"Departamento de Microbiolog\u00eda, Instituto Polit\u00e9cnico Nacional, Escuela Nacional de Ciencias Biol\u00f3gicas","place":["Ciudad de Mexico, Mexico"]},{"name":"Posgrado en Ciencias Quimicobiol\u00f3gicas, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Ciudad de Mexico, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/00nzavp26","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Investigaci\u00f3n en Inmunoqu\u00edmica, Hospital Infantil de M\u00e9xico Federico G\u00f3mez","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5299-759X","authenticated-orcid":false,"given":"Sara A.","family":"Ochoa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00nzavp26","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de M\u00e9xico Federico G\u00f3mez","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4090-455X","authenticated-orcid":false,"given":"Daniela","family":"De La Rosa-Zamboni","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiolog\u00eda, Hospital General de M\u00e9xico Dr Eduardo Liceaga","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1835-6788","authenticated-orcid":false,"given":"Silvia","family":"Giono-Cerezo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Instituto Polit\u00e9cnico Nacional, Escuela Nacional de Ciencias Biol\u00f3gicas","place":["Ciudad de Mexico, Mexico"]},{"name":"Posgrado en Ciencias Quimicobiol\u00f3gicas, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2577-9973","authenticated-orcid":false,"given":"Juan","family":"Xicohtencatl-Cortes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00nzavp26","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Investigaci\u00f3n en Bacteriolog\u00eda Intestinal, Hospital Infantil de M\u00e9xico Federico G\u00f3mez","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8018-1275","authenticated-orcid":true,"given":"Ariadnna","family":"Cruz-C\u00f3rdova","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00nzavp26","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Investigaci\u00f3n en Inmunoqu\u00edmica, Hospital Infantil de M\u00e9xico Federico G\u00f3mez","place":["Ciudad de Mexico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9040205"},{"key":"e_1_3_5_3_2","unstructured":"Red Hospitalaria de Vigilancia Epidemiologica. 2024. 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