{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:13:40Z","timestamp":1788225220864,"version":"build-2803163510"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["RO1 DE013986"],"award-info":[{"award-number":["RO1 DE013986"]}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["RO1 GM127909"],"award-info":[{"award-number":["RO1 GM127909"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,10,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oral bacteria can influence the ability of\n                    <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                    to cause oropharyngeal candidiasis (OPC). We recently reported that a\n                    <jats:italic toggle=\"yes\">Lactobacillus johnsonii<\/jats:italic>\n                    -enriched oral microbiota reduced\n                    <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                    virulence in an immunosuppressed OPC mouse model. As a follow-up, in this work, we aimed to enrich the resident oral\n                    <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                    communities with a prebiotic diet to further assess their effect on the severity of OPC. We tested the effect of a prebiotic xylo-oligosaccharides (XOS)-enriched diet in the oral global bacterial composition and severity of OPC. We assessed changes in the oral microbiome composition via 16S-rRNA gene high-throughput sequencing, validated by qPCR. The impact of the prebiotic diet on\n                    <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                    infection was assessed by quantifying changes in oral fungal and bacterial biomass and scoring tongue lesions. Contrary to expectations, oral\n                    <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                    communities were not enriched by the XOS-supplemented diet. Yet, XOS modulated the oral microbiome composition, increasing\n                    <jats:italic toggle=\"yes\">Bifidobacterium<\/jats:italic>\n                    abundance and reducing enterococci and staphylococci. In the OPC model, the XOS diet attenuated\n                    <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                    virulence and bacterial dysbiosis, increasing lactobacilli and reducing enterococci on the oral mucosa. We conclude that XOS attenuates\n                    <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                    virulence by promoting a bacterial microbiome structure more resilient to\n                    <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                    infection.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This is the first study on the effects of a prebiotic diet on the oral mucosal bacterial microbiome and an oropharyngeal candidiasis (OPC) mouse model. We found that xylo-oligosaccharides change the oral bacterial community composition and attenuate OPC. Our results contribute to the understanding of the impact of the oral bacterial communities on\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      virulence.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01734-23","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T09:02:53Z","timestamp":1693990973000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["A prebiotic diet modulates the oral microbiome composition and results in the attenuation of oropharyngeal candidiasis in mice"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3847-1305","authenticated-orcid":false,"given":"Roberto","family":"Vazquez-Munoz","sequence":"first","affiliation":[{"name":"Department of General Dentistry, The University of Connecticut Health Center","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, The University of Connecticut Health Center","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Sobue","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, The University of Connecticut Health Center","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dongari-Bagtzoglou","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, The University of Connecticut Health Center","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2016.02.022"},{"key":"e_1_3_4_3_2","first-page":"3","volume-title":"Candida and Candidiasis","author":"Calderone RA.","year":"2002","unstructured":"Calderone RA. 2002. Introduction and historical perspectives, p. 3\u201314. In Calderone, RA (ed.), Candida and Candidiasis, 1st ed. ASM Press, Washington, DC, USA.","edition":"1"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120452"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13479"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007717"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135404"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12216"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.122061"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.15620\/cdc:82532"},{"key":"e_1_3_4_11_2","unstructured":"NCCIH . 2023. Probiotics: what you need to know. Retrieved 22 January 2023. Available from: https:\/\/www.nccih.nih.gov\/health\/probiotics-what-you-need-to-know"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7010057"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiac095"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01937-21"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00089-23"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.853762"},{"key":"e_1_3_4_17_2","unstructured":"NCCIH . 2022. Probiotics: what you need to know. Retrieved 2 December 2022. 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