{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:55:28Z","timestamp":1790690128498,"version":"4.1.0"},"reference-count":9,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Burkholderia cenocepacia<\/jats:italic>\n                      is a formidable opportunistic pathogen, particularly in individuals with cystic fibrosis, yet its study has long been hampered by limited availability of genetic tools due to its high intrinsic antibiotic resistance. A recent study by M. M. Noe, K. Meesilpavikkai, Y. Ajimathorn, A. Sawatpanich, et al. (Microbiol Spectr 14:e02492-25, 2026,\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/spectrum.02492-25\" xlink:type=\"simple\">https:\/\/doi.org\/10.1128\/spectrum.02492-25<\/jats:ext-link>\n                      ) addresses this bottleneck by adapting a unified trimethoprim-selectable toolkit for markerless gene deletion and site-specific chromosomal complementation. By applying these tools to the\n                      <jats:italic toggle=\"yes\">purM<\/jats:italic>\n                      gene, the authors demonstrate that while\n                      <jats:italic toggle=\"yes\">purM<\/jats:italic>\n                      is universally essential for\n                      <jats:italic toggle=\"yes\">de novo<\/jats:italic>\n                      purine biosynthesis, its role in intracellular survival is strain-dependent. These findings highlight the metabolic heterogeneity within the\n                      <jats:italic toggle=\"yes\">Burkholderia cepacia<\/jats:italic>\n                      complex and provide a robust methodological framework for future functional genomics to help elucidate the metabolism, fitness, and virulence of these important bacteria. This commentary discusses how these advancements simplify complex genetic workflows and open new avenues for investigating the metabolic determinants of\n                      <jats:italic toggle=\"yes\">Burkholderia<\/jats:italic>\n                      pathogenesis and fitness.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01751-26","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:44Z","timestamp":1790686844000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Bridging a genetic gap with new tools to study the metabolism, fitness, and virulence in\n                    <i>Burkholderia cenocepacia<\/i>"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6979-2275","authenticated-orcid":true,"given":"Silvia","family":"Buroni","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00s6t1f81","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology and Biotechnology \u201cL. Spallanzani\u201d, University of Pavia","place":["Pavia, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1085"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02492-25"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2008.01576.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03024-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03909-14"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00699-24"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-021-11667-3"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.12.007"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01313-09"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01751-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01751-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:00:45Z","timestamp":1790686845000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01751-26"}},"subtitle":[],"editor":[{"given":"Varsha","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":9,"alternative-id":["10.1128\/spectrum.01751-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01751-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]},"assertion":[{"value":"2026-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01751-26"}}