{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:23:58Z","timestamp":1792034638588,"version":"4.4.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Huadong Medicine Joint Funds of the Zhejiang Provincial Natural Science Foundation of China","award":["LHDMY24H270002"],"award-info":[{"award-number":["LHDMY24H270002"]}]},{"name":"Zhejiang Provincial Medical and Health Technology Project","award":["2023KY158"],"award-info":[{"award-number":["2023KY158"]}]},{"name":"The Construction of Key Projects by Zhejiang Provincial Ministry","award":["WKJ-ZJ-2302"],"award-info":[{"award-number":["WKJ-ZJ-2302"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Pulmonary tuberculosis (PTB) and diabetes mellitus (DM) are prevalent chronic diseases with substantial implications for human health. DM patients are more susceptible to PTB, which exacerbates diabetes-related complications. However, the complex molecular mechanisms underlying the enhanced susceptibility of DM patients to PTB infection remain poorly understood. In this study, \u03b1- and \u03b2-diversity of gut microbiota was significantly reduced in PTB patients and PTB\u2013DM patients. The abundances of families\n              <jats:italic toggle=\"yes\">Lachnospiraceae<\/jats:italic>\n              and\n              <jats:italic toggle=\"yes\">Ruminococcaceae<\/jats:italic>\n              in the the\n              <jats:italic toggle=\"yes\">Firmicutes<\/jats:italic>\n              phylum were reduced in PTB patients and further diminished in PTB\u2013DM patients. On the other hand, untargeted metabolomics in frozen serum and stool samples indicated that phenylalanine, tyrosine, and tryptophan biosynthesis, metabolites of arginine, proline, tryptophan, and histidine were consistently altered in PTB patients and PTB\u2013DM patients, with significant upregulation of most metabolites. Amino acids like serine, proline, and histidine were both remarkably elevated in PTB and PTB\u2013DM patients. The correlation network analysis reveals the relationships between the shared microbial biomarkers and the shared metabolic pathways. This research contributes to the exploration of pivotal diagnostic biomarkers for both patients with PTB and PTB accompanied by diabetes. Specifically, shared reductions were identified in the genera\n              <jats:italic toggle=\"yes\">g-Roseburia<\/jats:italic>\n              ,\n              <jats:italic toggle=\"yes\">g-Ruminococcaceae_UCG.013<\/jats:italic>\n              ,\n              <jats:italic toggle=\"yes\">g-Ruminococcaceae_NK4A214<\/jats:italic>\n              ,\n              <jats:italic toggle=\"yes\">g-Lachnospiraceae<\/jats:italic>\n              _\n              <jats:italic toggle=\"yes\">unclassified<\/jats:italic>\n              , and\n              <jats:italic toggle=\"yes\">g-Firmicutes<\/jats:italic>\n              _\n              <jats:italic toggle=\"yes\">unclassified<\/jats:italic>\n              in addition to notable regulation of amino acids, like glycine, serine, and histidine in patients with PTB and PTB\u2013DM. Our study expands the comprehension of the intricate connections linking gut microbiota, fecal metabolites, and serum metabolites in PTB and PTB\u2013DM patients.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>This study expands the understanding of the complex links between gut microbiota, fecal metabolites, and serum metabolites in patients with PTB and PTB-DM through multi-omics techniques. It is helpful for us to understand the complex molecular mechanism of increased susceptibility to PTB infection in diabetic patients.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.01772-24","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T09:01:18Z","timestamp":1741942878000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Disorders of gut microbiota and fecal\u2013serum metabolic patterns are associated with pulmonary tuberculosis and pulmonary tuberculosis comorbid type 2 diabetes mellitus"],"prefix":"10.1128","volume":"13","author":[{"given":"Yunguang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)","place":["Hangzhou, China"]},{"name":"Zhejiang Key Laboratory of Research and Translation for Kidney Deficiency-Stasis-Turbidity Disease","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)","place":["Hangzhou, China"]},{"name":"Zhejiang Key Laboratory of Research and Translation for Kidney Deficiency-Stasis-Turbidity Disease","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)","place":["Hangzhou, China"]},{"name":"Zhejiang Key Laboratory of Research and Translation for Kidney Deficiency-Stasis-Turbidity Disease","place":["Hangzhou, 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