{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:43:02Z","timestamp":1792417382820,"version":"4.4.0"},"reference-count":33,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100012319","name":"National Center for Global Health and Medicine","doi-asserted-by":"publisher","award":["22A1007"],"award-info":[{"award-number":["22A1007"]}],"id":[{"id":"10.13039\/100012319","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Japan Society for the Promotion of Science","award":["JP19K19450"],"award-info":[{"award-number":["JP19K19450"]}]},{"name":"Japan Society for the Promotion of Science","award":["JP22K17408"],"award-info":[{"award-number":["JP22K17408"]}]},{"name":"Shionogi & Co., Ltd"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                      species are environmental bacteria occasionally implicated in human infections; however, their clinical and genomic features are not fully characterized. This study investigated the clinical, genotypic, and phenotypic characteristics of\n                      <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                      strains collected through the MultiDrug-Resistant Organisms Clinical Research Network, a multicenter prospective study of patients with carbapenem-resistant gram-negative bacilli isolated at tertiary care hospitals in Japan between April 2019 and March 2024. Among 18 patients with\n                      <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                      spp. confirmed by whole-genome sequencing (WGS), 5 had infection, and 13 were colonized. Bacteremia and pneumonia were the common types of infection. The isolates comprised five species, with\n                      <jats:italic toggle=\"yes\">Chryseobacterium indologenes<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Chryseobacterium culicis<\/jats:italic>\n                      being the most frequently detected. Compared with WGS, 16S rRNA gene sequencing demonstrated the highest species-level identification accuracy (93.8%), followed by MALDI Biotyper (50.0%), VITEK-MS (31.3%), and VITEK 2 Blue (31.3%). All systems correctly identified\n                      <jats:italic toggle=\"yes\">C. indologenes<\/jats:italic>\n                      , but other species were frequently misidentified or remained unidentified because of limited database coverage. All isolates exhibited broad \u03b2-lactam resistance, including resistance to carbapenems, which was associated with species-specific combinations of metallo-\u03b2-lactamase and extended-spectrum \u03b2-lactamase genes. Minocycline, trimethoprim-sulfamethoxazole, and cefiderocol retained activity across all species. These findings highlight that diverse\n                      <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                      species, including uncommon ones, can be associated with human infections. Incorporating genomic approaches such as WGS or 16S rRNA gene analysis can improve species-level identification, especially for rare or emerging species.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                        species are emerging opportunistic pathogens that pose a diagnostic and therapeutic challenge as they are intrinsically resistant to most \u03b2-lactams, including carbapenems, and are often misidentified by routine clinical laboratory systems. In this multicenter study, we show that clinical\n                        <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                        isolates represent greater species diversity than is typically recognized, extending beyond\n                        <jats:italic toggle=\"yes\">Chryseobacterium indologenes<\/jats:italic>\n                        to include uncommon species associated with both infection and colonization. Whole-genome sequencing clarified species assignments, revealed species-specific \u03b2-lactamase profiles, and highlighted the limitations of commonly used identification platforms, particularly for species absent from commercial databases. Despite broad \u03b2-lactam resistance, cefiderocol, minocycline, and trimethoprim-sulfamethoxazole retained\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        activity across species. These findings emphasize the need for genomic or sequence-based identification to accurately define the clinical spectrum of\n                        <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                        species, guide interpretation of antimicrobial susceptibility, and improve recognition of these organisms as emerging healthcare-associated pathogens.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01793-26","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T13:00:48Z","timestamp":1792414848000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Clinical and genomic features of\n                    <i>Chryseobacterium<\/i>\n                    species isolated from clinical 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