{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:23:43Z","timestamp":1788269023420,"version":"build-2803163510"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Toxigenic\n                      <jats:italic toggle=\"yes\">Klebsiella oxytoca<\/jats:italic>\n                      strains produce the pyrrolobenzodiazepine enterotoxins tilimycin (TM) and tilivalline (TV), which contribute to the development of antibiotic-associated hemorrhagic colitis. The biosynthesis of these toxins depends on the nonribosomal peptide synthetase (NRPS) operon located within the\n                      <jats:italic toggle=\"yes\">til<\/jats:italic>\n                      pathogenicity island. Although several global and signal-responsive regulators of NRPS operon expression have been identified, the regulatory network governing enterotoxin biosynthesis remains incompletely characterized. In this study, we identified a previously unrecognized transcriptional regulator encoded within the\n                      <jats:italic toggle=\"yes\">til<\/jats:italic>\n                      pathogenicity island of\n                      <jats:italic toggle=\"yes\">K. oxytoca<\/jats:italic>\n                      . This protein, designated TagR (Tilivalline-associated genes repressor), is a member of the MarR family and acts as a negative regulator of NRPS operon expression. Structural prediction, molecular dynamics simulations, and biochemical analyses demonstrated that TagR exhibits the characteristic architecture of MarR family regulators and forms a stable homodimer. Deletion of\n                      <jats:italic toggle=\"yes\">tagR<\/jats:italic>\n                      led to significant upregulation of the NRPS-associated genes\n                      <jats:italic toggle=\"yes\">npsA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">thdA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">npsB<\/jats:italic>\n                      , while complementation restored transcriptional repression. Electrophoretic mobility shift assays confirmed that TagR binds directly and specifically to the regulatory region upstream of the NRPS operon, supporting a mechanism of direct transcriptional repression. Consistent with these findings, loss of TagR significantly increased the cytotoxicity of\n                      <jats:italic toggle=\"yes\">K. oxytoca<\/jats:italic>\n                      culture supernatants toward HeLa cells. Collectively, these results identify TagR as a direct repressor of the NRPS operon and expand the regulatory framework governing enterotoxin biosynthesis in toxigenic\n                      <jats:italic toggle=\"yes\">K. oxytoca<\/jats:italic>\n                      . This study provides new insight into the transcriptional control of virulence-associated genes and establishes TagR as a previously unrecognized component of the regulatory network controlling TM and TV production.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Elucidating the mechanisms by which toxigenic\n                        <jats:italic toggle=\"yes\">Klebsiella oxytoca<\/jats:italic>\n                        regulates enterotoxin production is critical for understanding the pathogenesis of antibiotic-associated hemorrhagic colitis. TagR is identified as a previously unrecognized MarR family regulator that directly represses the nonribosomal peptide synthetase (NRPS) operon responsible for tilimycin (TM) and tilivalline (TV) biosynthesis. This discovery uncovers a novel regulatory mechanism governing toxin production and offers new perspectives on virulence regulation in this emerging intestinal pathogen.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01853-26","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:02:25Z","timestamp":1785934945000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["TagR, a newly identified member of the MarR family of transcriptional regulators, represses the NRPS operon in\n                    <i>Klebsiella oxytoca<\/i>"],"prefix":"10.1128","volume":"14","author":[{"given":"Cristopher","family":"Perez","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Posgrado en Ciencias en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario A.","family":"L\u00f3pez-Luis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Biotecnolog\u00eda y Boinform\u00e1tica Gen\u00f3mica, Departamento de Bioqu\u00edmica, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos J.","family":"Jim\u00e9nez-S\u00e1nchez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Posgrado en Ciencias en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferm\u00edn I.","family":"Hern\u00e1ndez-Pompa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Posgrado en Ciencias en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Rodr\u00edguez-Valverde","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Rivera-Guti\u00e9rrez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9519-8766","authenticated-orcid":true,"given":"Roberto","family":"Rosales-Reyes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Chimal-C\u00e1zares","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/00dmdt028","id-type":"ROR","asserted-by":"publisher"}],"name":"Divisi\u00f3n de Ciencias de la Salud, Universidad Tecnol\u00f3gica de Tulancingo","place":["Tulancingo, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Soria-Bustos","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03p2z7827","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Detecci\u00f3n Biomolecular, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","place":["Puebla, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge A.","family":"Ya\u00f1ez-Santos","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03p2z7827","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Detecci\u00f3n Biomolecular, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","place":["Puebla, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Cedillo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03p2z7827","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Detecci\u00f3n Biomolecular, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","place":["Puebla, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6909-2941","authenticated-orcid":true,"given":"Miguel A.","family":"De la Cruz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03p2z7827","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Detecci\u00f3n Biomolecular, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","place":["Puebla, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/03p2z7827","id-type":"ROR","asserted-by":"publisher"}],"name":"Facultad de Medicina, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla","place":["Puebla, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9307-6116","authenticated-orcid":true,"given":"James G.","family":"Fox","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/042nb2s44","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Comparative Medicine, Massachusetts Institute of Technology","place":["Cambridge, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2574-958X","authenticated-orcid":true,"given":"Miguel A.","family":"Ares","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xddgg98","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades infecciosas y Parasitarias, Hospital de Pediatr\u00eda, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social","place":["Mexico City, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/059sp8j34","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional","place":["Mexico City, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa054765"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1819154116"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.743594"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00780-24"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13010158"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1155\/ijm\/2094815"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms14051144"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03752-21"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1403274111"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20225595"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201707737"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.184101.114"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2020.01.002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00428"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1291172"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23588-w"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1370093"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2537530"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01244-3"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2017.06.001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2017.1344612"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01358"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.21775\/cimb.008.051"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt523"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189694"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01331-06"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817572.ch18"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3769"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01039"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00039-18"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20945"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0014475"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21787"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v035.b01"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot102228"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.12.013"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100542"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.791558"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(76)90119-4"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00389-n"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.07.004"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201100335"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13112591"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02429-14"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00530-07"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0157165"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp496"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02224-12"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.20.6195-6198.2003"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00648"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5456"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjq021"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11081936"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.09.013"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.07.002"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8040576"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00794.x"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.02065"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01853-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01853-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:09:29Z","timestamp":1788268169000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01853-26"}},"subtitle":[],"editor":[{"given":"Jan-Ulrik","family":"Dahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":66,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,1]]}},"alternative-id":["10.1128\/spectrum.01853-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01853-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"assertion":[{"value":"2026-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01853-26"}}